Method of potato virus eradication
A potato virus removal technology, applied in horticultural methods, botanical equipment and methods, plant preservation, etc., can solve the problem of unfavorable survival of shoot tips and regeneration of differentiated plants, low plant survival rate and virus-free rate, and difficulty in cutting The stem tip and other problems can be solved, and the effect of saving manpower and financial resources, less difficult operation and strong frost resistance
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[0012] Example 1
[0013] This example is to test the "Zao Dabai" potato variety. The specific operation method is: select potato materials, carry out propagation, and then perform virus detection on the materials, the detection method used is the RT-PCR method; and then establish a single bud Propagating and subculturing the established single bud line materials. Propagating the confirmed virus-bearing materials to prepare for ultra-low temperature storage. Use the subculture medium: MS basic medium + 0.2mol·L -1 6-BA+0.03mol·L -1 IAA conducts proliferation culture, the pH of the medium is 5.8, the temperature of the culture room is 22℃, and the light intensity is 40μmol·m -2 ·S -1 ,Lighting time is 12h·d -1 ; When the material is multiplied to a certain amount, it is stored in ultra-low temperature, that is, the vitrification method is adopted, and the exercise is carried out at a low temperature of 4 ℃ for 6 days, and then placed in a medium supplemented with dimethyl sulfoxide...
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[0014] Example 2
[0015] This example is to test the "Zhengshu No. 5" potato variety. The specific operation method is: select potato materials, carry out propagation, and then perform virus detection on the materials, and the detection method used is the RT-PCR method; and then establish Single bud line material, the established single bud line material is expanded and subcultured. The virus-bearing material is expanded and used for ultra-low temperature storage. The selection of the subculture medium: MS basic medium + 0.2mol·L -1 6-BA+0.03mol·L -1 IAA conducts proliferation culture, the pH of the medium is 5.8, the temperature of the culture room is 23℃, and the light intensity is 38μmol·m -2 ·S -1 ,Lighting time is 13h·d -1 ; When the material is multiplied to a certain amount, it is stored in ultra-low temperature, that is, the vitrification method is adopted, and it is exercised at a low temperature of 3°C for 7 days, and then placed in a medium supplemented with dimethyl s...
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