The invention relates to a novel single-layer cutting-free bag flower mushroom cultivation process which carries out flower mushroom cutting-free cultivation by a plastic degradation modified cylinderbag of which the single layer is specially processed. After a culture material is charged into the bag to be carried out the phases of disinfection, bacterial fermentation, culture, and the like, a bacterial rod surface film similar to fruiting film ruptured force can be gradually formed; in the fruiting phase, a flower mushroom can directly rupture the film (bag) and fruit by self growing force,the natural growing environments of automatic film rupture and wet inside and dry outside are created for the flower mushroom; the waste of a plurality of plastic resources caused by double-layer bagcultivation is reduced, the white pollution is lowered, and the working procedures of two-step packaging, discoloured water necrosis and decay of an interlayer of two bags, outer bag head cutting after bacterial fermentation maturity, outer bag removal before fruiting, and the like in the double-layer bag cultivation and the bacterial rod loss and mushroom damage caused by the working proceduresare eliminated, thereby achieving the aims of saving power and cost, improving the quality of the flower mushroom and protecting the ecological environment.