Feed additive with antibiotic function and application

A feed additive and functional technology, applied in the field of feed additives and applications, can solve the problems of low content of natural antimicrobial peptides, high price of chemically synthesized peptides, and inability to obtain large quantities, so as to achieve good antibacterial effects, increase immune and disease resistance functions, and solve the problem of antibiotics Cumulative Exceeding Effects

Inactive Publication Date: 2008-04-23
THE FIRST INST OF OCEANOGRAPHY SOA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although antimicrobial peptides have remarkable antibacterial efficacy and broad application prospects, the content of natural antimicrobial peptides in organisms is very low and cannot be obtained in large quantities; chemically synthesized peptides are expensive, which limits their wide application

Method used

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  • Feed additive with antibiotic function and application
  • Feed additive with antibiotic function and application
  • Feed additive with antibiotic function and application

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Embodiment Construction

[0040] Below with embodiment and in conjunction with accompanying drawing, content of the present invention will be further described:

[0041] 1. Preparation of turbot liver cDNA: 24 hours after turbot was injected with Vibrio anguillarum, the liver was taken out, quickly placed in liquid nitrogen, and the hard tissue pieces were frozen at -80°C for preservation. Refer to TRIZOL  Reagent operation instructions, take about 100mg of giant flounder tissue, add it to 1ml TRIZOL reagent, homogenize at room temperature for 5 minutes, add 200μl chloroform to shake vigorously for 15 seconds, and incubate at room temperature for 3 minutes. Centrifuge at 12,600 rpm for 15 min at 4°C. Add 250μl of high-concentration salt solution composed of 0.8M sodium acetate and 1.2M sodium chloride to obtain the upper layer of the interface, mix well, then add 250μl isopropanol and mix well, settle at room temperature for 10min, and then rotate at 12,600rpm at 4℃ Centrifuge for 10 min. After the...

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PUM

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Abstract

The present invention discloses a feed additive with anti-bacterial function and its application. The described feed additive is a fusion protein formed from modified fishes antibacterial peptide hepecidin and amphibian antibacterial peptide magainin, in which said fusion protein contains short peptide of amino acid sequence indicated by SEQ ID No.1 or the described feed additive is characterized by that in its genome one of Pichia yeast, brewer's yeast and candida of nucleotide sequence indicated by SEQ ID No.2 or their any combination is contained, the number of the described saccharomycetes is greater than 5X104 cfu/g, and the water content is less than or equal to 10%. The invented feed additive can be extensively used for preparing feed with the function for preventing and curing diseases for aquatic creature or domestic poultry and livestock, etc.

Description

technical field [0001] The invention relates to a feed additive and its application, in particular to an animal antimicrobial peptide gene carrier established by genetic engineering technology and a feed additive obtained through a yeast expression system and its application. Background technique [0002] With the rapid development of my country's animal husbandry and aquaculture intensive farming mode and the increasing environmental pollution, high-density farming makes livestock and aquatic organisms more and more likely to be infected with diseases, and diseases occur frequently, often causing relatively large Economic losses. The disease problem has become a bottleneck restricting the development of the breeding industry. Traditionally, the main countermeasures taken against breeding diseases are the use of antibiotics and other types of chemical drugs, which inevitably brings about the problems of bacterial drug resistance and drug residues. Extensive use of antibioti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/17A23K20/195
CPCY02A40/70
Inventor 丛柏林林学政刘晨临刘胜浩黄晓航
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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