Inserting-plate type microalgae semi-dry solid adherent culture device

A semi-dry solid-state, adherent culture technology, applied in solid-phase fermentation bioreactors and other directions, can solve the problems of high energy consumption, large consumption of nutrient salts, and low utilization of light energy in large water bodies for stirring and mixing, and achieve cell growth. The state is easy to control, easy to add and remove, and the effect of high utilization of light energy

Inactive Publication Date: 2013-09-11
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the large water body, it has caused great difficulties to improve the efficiency of microalgae cultivation, reduce the cost and scale up the system: (1) The algae cells reflect and block the light, and the attenuation of light in the large water body is serious, and the algae cells cannot maximize Utilize sunlight; 2) The large water body consumes huge energy for maintaining the temperature of the culture system; 3) The stirring and mixing energy consumption of the large water body is high; 4) The large water body is too large to cause nutrients and CO 2 Large consumption; 5) The concentration of cells in large water bodies is low, and it is difficult to harvest algae cells; 6) The large volume, heavy weight, and high pressure brought by large water bodies are the reasons for realizing the large-scale traditional culture equipment and improving space utilization under the current technical conditions. formidable obstacle
For example, transparent materials such as glass, plexiglass, and plastic film used in traditional photobioreactors are not suitable for large-scale and space-height amplification due to their low mechanical strength. The energy utilization rate is low, and this is the most important direct constraint factor for the industrialization of large-scale cultivation of microalgae
[0005] It can be said that the culture methods used so far for microalgae have failed to take advantage of the advantages of high light efficiency and high biomass yield of microalgae, and the production cost is relatively high
Aiming at the low efficiency of microalgae large-scale cultivation, people have made many improvements in terms of equipment structure, carbon replenishment methods, and operation modes, but they have failed to greatly improve the photosynthetic utilization efficiency of microalgae, yield per unit area, and reduce costs. fundamental breakthrough

Method used

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  • Inserting-plate type microalgae semi-dry solid adherent culture device

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Five culture plates 2 are placed on a support truss 1 made of stainless steel. Each culture plate 2 is 40 cm long, 70 cm high, and 3 cm thick. The culture plate 2 is a multilayer polyurethane sponge composite. The culture plate 2 is covered with stainless steel except for the bottom edge. A double-sided stainless steel clip strip is installed on each culture plate 2 as a harvesting scraper. The distance between two adjacent culture plates 2 is 20 cm. The reservoir 9 is filled with Spirulina platensis (commercially available) culture medium (composition is per 1 liter of culture medium containing: 16.80 grams of sodium bicarbonate, 0.5 grams of potassium hydrogen phosphate, 2.5 grams of sodium nitrate, and sodium chloride 1.0g, 0.2g magnesium sulfate, 0.01g iron sulfate, 1.0g potassium sulfate, 0.04g calcium chloride monohydrate, 0.08g EDTA, adjust pH to 8.0 with NaOH solution). Spray the pre-cultured spirulina seed solution on the surface of the culture plate 2. The ...

Embodiment 2

[0035] Five culture plates 2 are placed on a stainless steel support truss 1, and each culture plate 2 is 40 cm long, 70 cm high, and 2.9 cm thick. The middle sandwich layer of the culture plate 2 is a polyurethane sponge with a thickness of 2.5 cm. The two outer layers of the sandwich layer are flocked cloth. The culture plate 2 is covered with stainless steel except for the bottom edge. A double-sided stainless steel clip strip is installed on each culture plate 2 as a harvesting scraper. The distance between two adjacent culture plates 2 is 20cm. The liquid storage bottle 9 is filled with Spirulina platensis (commercially purchased) medium (composition is that each 1 liter of medium contains: 16.80 grams of sodium bicarbonate, phosphoric acid Potassium hydrogen 0.5 g, sodium nitrate 2.5 g, sodium chloride 1.0 g, magnesium sulfate 0.2 g, iron sulfate 0.01 g, potassium sulfate 1.0 g, calcium chloride monohydrate 0.04 g, EDTA 0.08 g, adjust pH to 8.0 with NaOH solution ). S...

Embodiment 3

[0037] Five culture plates 2 are placed on a support truss 1 made of stainless steel. Each culture plate 2 is 40 cm long, 70 cm high, and 3 cm thick. The culture plate 2 is a polyurethane sponge, and the distance between two adjacent culture plates 2 is 20 cm. The reservoir 9 is filled with 10 liters of Spirulina platensis (commercially available) culture medium (composition of each 1 liter of culture medium contains: 16.80 grams of sodium bicarbonate, 0.5 grams of potassium hydrogen phosphate, 2.5 grams of sodium nitrate, chlorine Sodium sulfide 1.0 g, magnesium sulfate 0.2 g, iron sulfate 0.01 g, potassium sulfate 1.0 g, calcium chloride monohydrate 0.04 g, EDTA 0.08 g, pH 8.0 adjusted with NaOH solution). Spray the pre-cultured spirulina seed liquid on the surface of the culture plate 2. The supporting truss 1 is wrapped with transparent plastic film around and on the top, the device is placed outdoors, and the sunlight intensity at noon is 1500 μmol / m 2 / s, about 500μmol / m...

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Abstract

The invention belongs to the field of microalgae culture, and particularly relates to an inserting-plate type microalgae semi-dry solid adherent culture device. The inserting-plate type microalgae semi-dry solid adherent culture device is characterized by comprising a supporting truss, culture plates, liquid distributing pipes, liquid collecting tanks and a liquid storage bottle, wherein the supporting truss is provided with at least one culture plate, and one liquid distributing pipe is arranged above each culture plate; the liquid collecting tank arranged on the supporting truss is arranged under each culture plate; all the liquid collecting tanks are communicated with the liquid storage bottle by the main liquid pipe; the bottom of the liquid storage bottle is respectively provided with a circulating pump and a carbon-supplementing gas distributing pipe communicated with a carbon dioxide source, and the circulating pump is communicated with a liquid distributing pipe by a pipeline; and each culture plate is provided with a picking scraper. The inserting-plate type microalgae semi-dry solid adherent culture device has the advantages that the yield in unit occupied area is effectively increased, the culture period is shortened, the water consumption for culture is greatly reduced, the cost and the operating cost of the culture device are reduced and the industrial culture on large scale is easily realized.

Description

Technical field [0001] The invention belongs to the field of microalgae cultivation, and specifically is a plate-inserting type microalgae semi-dry solid adherent culture device. Background technique [0002] Microalgae refers to aquatic algae capable of photosynthesis. Certain microalgae are rich in protein. Under certain conditions, certain microalgae can synthesize a large amount of secondary metabolites, such as oils, hydrocarbons, carotenoids, starch, polysaccharides, etc., which can be used to produce functional foods, food additives, and medical insurance. Key products, protein, feed, bioenergy such as biodiesel, aviation kerosene, etc. At the same time, due to the fast growth of microalgae and high photosynthetic efficiency, it absorbs a large amount of CO during photosynthetic growth. 2 Therefore, microalgae is considered to be the most promising biomass resource with excellent carbon sequestration and emission reduction effects. Globally, a biotechnology industry chai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/16
Inventor 刘天中王俊峰陈昱陈晓琳高莉丽陈林纪蓓刘金丽
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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