Culture method for tricholoma matsutake liquid strain

A liquid strain and culture method technology, applied in the biological field, can solve the problems of lack of control and management in the cultivation process, uneven distribution of nutrients, cumbersome cultivation steps, etc., and achieve favorable absorption and utilization, lower utilization rate, and good cultivation effect Effect

Inactive Publication Date: 2016-04-20
宋瑞鹏
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Problems solved by technology

[0002] Matsutake is a precious and rare wild edible fungus with high nutritional value. At present, in the artificial cultivation, matsutake strains still adopt the original cultivation method. The cultivation process lacks effective control and management, and the distribution of nutrients is uneven. Among the matsutake strains The cultivation process needs to go through multiple steps such as inoculation, static cultivation and shake flask cultivation, and it is also necessary to artificially inject sterile air into the culture vessel, resulting in cumbersome cultivation steps and high labor intensity.

Method used

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  • Culture method for tricholoma matsutake liquid strain

Examples

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Embodiment 1

[0034] 200 grams of pine branches, 200 grams of potatoes and 1500 mL of water were mixed, boiled and kept for 30 minutes, then filtered and cooled to obtain the filtrate;

[0035] Add carbon source and nitrogen source to 1000mL of the filtrate, after fully dissolving, take 150mL and put it into a culture bottle, then add 15 grams of washed and dried chaff, 0.3 gram of KH 2 PO 4 , 0.3 g MgSO 4 and 0.5 gram of citric acid, mixed evenly to obtain matsutake liquid culture medium;

[0036] After the culture bottle containing the matsutake liquid strain culture medium is sterilized at high temperature in a sterilizing pot and cooled, it is placed in a sealed aseptic inoculation box, and the matsutake original species is inserted into the culture bottle, and placed Cultivate in an incubator at 24°C. After the mycelium of the original matsutake seed germinates, shake the matsutake liquid strain culture medium once every morning at 9 o'clock, and cultivate for a certain period of tim...

Embodiment 2

[0060] This embodiment provides a method for culturing matsutake liquid strains, the specific cultivation steps are roughly the same as in Example 1, the difference is that the specific cultivation steps of matsutake liquid strains include:

[0061] (1) Preparation of medium for matsutake parent species and cultivation of matsutake parent species

[0062] Add 50 grams of potatoes, 8 grams of agar, 0.5 grams of peptone, and 5 grams of glucose into 1500 mL of water and heat in turn until all the agar is boiled to obtain matsutake mother seed medium.

[0063] Take 150 mL of the matsutake mother seed culture medium and place it in a culture bottle, seal it with cotton, place it in a pressure cooker at a temperature of 130° C. for sterilization for 2 hours, and then cool it naturally to room temperature. Place the culture bottle in a sealed aseptic inoculation box, insert the hyphae of the matsutake strain into the culture bottle, place it in an incubator at 28° C., and cultivate u...

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Abstract

The invention provides a culture method for a tricholoma matsutake liquid strain. The culture method includes the steps that a culture medium for the tricholoma matsutake liquid strain is prepared, the culture medium for the tricholoma matsutake liquid strain is inoculated with a tricholoma matsutake stock seed for sealing culture, and after hyphae of the tricholoma matsutake stock seed germinate, the culture medium for the tricholoma matsutake liquid strain is shaken once every day until the tricholoma matsutake liquid strain is obtained. The culture method for the tricholoma matsutake liquid strain is easy to operate, cultured richoloma matsutake liquid strain hyphae grow fast and are contaminated by other strains little, the hypha output rate is high, and cost is low.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for cultivating matsutake liquid strains. Background technique [0002] Matsutake is a precious and rare wild edible fungus with high nutritional value. At present, in the artificial cultivation, matsutake strains still adopt the original cultivation method. The cultivation process lacks effective control and management, and the distribution of nutrients is uneven. Among the matsutake strains The cultivation process needs multiple steps such as inoculation, static cultivation and shake flask cultivation, and it is also necessary to artificially inject sterile air into the culture vessel, resulting in cumbersome cultivation steps and high labor intensity. [0003] In order to solve the above existing problems, people have been seeking an ideal technical solution. Contents of the invention [0004] In view of this, the present invention clearly needs to provide a method for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 宋瑞鹏
Owner 宋瑞鹏
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