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A method for artificially cultivating white waxy caps

A technology of artificial cultivation and ash, which is applied in cultivation, plant cultivation, mushroom cultivation, etc. It can solve the problems of high requirements for the growth conditions of ash umbrella and unsuccessful ash umbrella cultivation, and achieve great research and development value and high cultivation efficiency. Effect

Active Publication Date: 2015-11-18
贾富海
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The problem is that ash umbrellas have high requirements for growth conditions, and slight changes or differences in temperature and humidity will lead to unsuccessful cultivation of ash umbrellas.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0064] 1. Collect the fruiting bodies of the ash umbrella.

[0065] 2. Remove debris on the surface of the fruiting body, disinfect the surface with 75% alcohol, use sterile tweezers to pick up 5 mm × 3 mm × 3 mm bacterial tissue and transfer it to the culture medium of the inclined test tube of the mother species, plug it with a cotton plug, and place it at a constant temperature of 5 °C White hairy hyphae can be seen on the tissue block after being cultivated for 5 days; the hairy mycelium is covered with slant test tubes in 18 days to obtain a first-class strain; 2 grams of maltose, 2 grams of glucose, 0.5 grams of magnesium sulfate, 0.5 grams of calcium sulfate, 0.25 grams of potassium dihydrogen phosphate, 20 grams of agar, 1000 ml of distilled water, and adjust the pH value to 7.5.

[0066] 3. Secondary strain cultivation: Soak oat grains and miscellaneous sawdust in cold water for 12 hours, control to dry, steam in a pot for 30 minutes, cool down to 15-20°C, add sucrose...

example 2

[0085] 1. Collect the fruiting bodies of the ash umbrella.

[0086] 2. Remove debris on the surface of the fruit body, disinfect the surface with 75% alcohol, use sterile tweezers to pick up 5 mm × 3 mm × 3 mm bacterial tissue and transfer it to the culture medium of the parent inclined test tube, plug it with a cotton plug, and place it at a constant temperature of 36 ° C. After 5 days of culture, white hairy hyphae can be seen on the tissue block; after 18 days, the hairy mycelium is covered with inclined test tubes, and the first-class strain is obtained.

[0087] The formula of the above-mentioned mother species test tube slant medium is 30 grams of malt extract, 8 grams of potassium dihydrogen phosphate, 6 grams of potassium carbonate, 25 grams of agar, 1000 ml of distilled water, and adjust the pH to 7.5.

[0088] 3. Secondary strain cultivation: Soak oat grains and miscellaneous sawdust in cold water for 12 hours, control to dry, steam in a pot for 30 minutes, cool down...

example 3

[0107] 1. Collect the fruiting bodies of the ash umbrella.

[0108] 2. Remove debris on the surface of the fruit body, disinfect the surface with 75% alcohol, use sterile tweezers to pick up 5 mm × 3 mm × 3 mm bacterial tissue, transfer it to the culture medium of the mother species' inclined test tube, plug it with a cotton plug, and place it at a constant temperature of 20 ° C. After 5 days of culture, white hairy hyphae can be seen on the tissue block; after 16 days, the hairy mycelium is covered with inclined test tubes, and the first-class strain is obtained.

[0109] The formula (2) of the above-mentioned female seed test tube slant medium: 30 grams of malt extract, 8 grams of potassium dihydrogen phosphate, 6 grams of potassium carbonate, 25 grams of agar, 1000 ml of distilled water, and adjust the pH to 6.5.

[0110] 3. Secondary strain cultivation: Soak oat grains and miscellaneous sawdust in cold water for 12 hours, control to dry, steam in a pot for 30 minutes, cool...

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PUM

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Abstract

The invention relates to a method for artificially cultivating white waxy caps. The white waxy cap is high in trace element content and is thus listed as a superfine product in fungi. The method realizes artificial cultivation of the white waxy cap fungi by utilizing white waxy cap sporocarps collected in the field, by reasonably configuring first-stage, second-stage and third-stage fungus cultivation formulas and by controlling the factors of nutrition, temperature, humidity, illumination, air environment and pH value and the like in the fungus cultivation process; and the method has higher cultivation efficiency and has larger research and development values.

Description

technical field [0001] The invention relates to a method for artificially cultivating ash umbrellas. The content of trace elements in the ash umbrella is high, so it is listed as the best among bacteria. The invention realizes the artificial cultivation of the white wax agaric species, has higher cultivation efficiency, and has greater research and development value at the same time. Background technique [0002] It is recorded on page 67 of "Chinese Tan Fungus" by Beijing Science Publishing House, Chinese alias, ivory white wax umbrella; Latin literary name is Hygrophoruse burnesus (Bull.) Fr.; Japanese name, white sticky umbrella; English name is whitewaxycap, ivorywaxycap. The ash umbrella body is small, white, with a cap diameter of 2 to 8 cm, flat hemispherical to flat, white, yellowish or pinkish, smooth. Bacteria flesh-colored. Gills subextensive, sparse, stipe 5-13CM long, 0.3-1.5CM thick, subgranular, bottom tapering, smooth, with scales on top, spores colored, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C12N1/14
CPCA01G18/00C12N1/14
Inventor 贾富海
Owner 贾富海
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