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Method for domesticating prairie Tricholomataceae and method for cultivating the same

A technology of prairie white mushrooms, cultivation methods, applied in the fields of botany equipment and methods, climate change adaptation, horticulture, etc.

Inactive Publication Date: 2004-11-17
方在吉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no technology in China that can realize the artificial cultivation of grassland white mushrooms.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Under aseptic conditions, cut open the fruiting bodies of fresh wild prairie white mushrooms, extract a small piece of fungus meat tissue, and move them into the chamber at a temperature of 121-126°C and a pressure of 1.1-1.5kg / cm 2 On the slant of the PDA medium test tube containing 180 grams of potatoes, 180 grams of glucose, 200 grams of agar and 100 ml of water, which were sterilized and maintained for 30 minutes, placed in a 26-28°C incubator and cultivated for 30 days. After the hyphae are all over the slope of the test tube, expand the parent species cultured in the PDA medium test tube and put it into the test tube at a temperature of 121-126°C and a pressure of 1.1-1.5kg / cm 2 In the test tube of sawdust bran culture medium containing miscellaneous sawdust 73%, bran 15%, sucrose 1%, gypsum powder 1% and material-water ratio of 1: 1.2, maintained for 30 minutes under the condition of sterilization, at 26~ Cultured in a 28°C incubator for 30 days. After the mycel...

Embodiment 2

[0032] Under aseptic conditions, cut open the fruiting bodies of fresh wild prairie white mushrooms, extract a small piece of fungus meat tissue, and move them into the chamber at a temperature of 121-126°C and a pressure of 1.1-1.5kg / cm 2 On the slant of the PDA medium test tube containing 200 grams of potatoes, 200 grams of glucose, 200 grams of agar and 100 ml of water, which were sterilized and maintained for 30 minutes, placed in a 26-28°C incubator and cultivated for 35 days. After the hyphae are all over the slope of the test tube, expand the parent species cultured in the PDA medium test tube and put it into the test tube at a temperature of 121-126°C and a pressure of 1.1-1.5kg / cm 2In the test tube of the sawdust bran medium containing 78% miscellaneous sawdust, 20% bran, 1% sucrose, 1% gypsum powder and a ratio of material to water of 1:1.3, maintained for 30 minutes under the condition of Cultured in a 28°C incubator for 35 days. After the mycelia cover the slope o...

Embodiment 3

[0034] Stack the crushed and pre-wetted 55% cow dung and 43% straw layer by layer, start watering the fourth layer, add 1% gypsum powder and 1% calcium superphosphate from the fourth layer to the eighth layer, and top Cover with a layer of dung, the top of the pile is turtle-shaped, and adjust the amount of water, so that it is advisable to see a little overflow at the bottom. On the second day of stockpiling, the material temperature is controlled to about 65°C, on the third day the material temperature is 75-80°C, and the first turning is carried out after 7 days. Turn the pile for the second time after 6 days, and keep the material temperature at 60-65°C. Turn the pile for the third time after 5 days, turn the pile for the fourth time after 4 days, and turn the pile for the fifth time after 3 days. At this time, the temperature of the material is 50 ℃, then adjust the pH value to 7.6, and stop fermentation after another 3 days.

[0035] Put the culture material on the mush...

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PUM

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Abstract

The invention discloses a white grassland mushroom domestication method and cultivation process, wherein the white grassland mushroom original breed is obtained through the steps of, selecting different culture medium and culture condition, making female seeds, female seeds culturing, and making original breed as a domestication procedure, and then the cultivation is realized through controlling the culture condition of temperature and humidity.

Description

technical field [0001] The invention relates to the technical field of cultivation of edible fungi, in particular to a domestication method and a cultivation method of white mushrooms in grasslands. Background technique [0002] Grassland white mushroom, also known as grassland white mushroom, Hulunbuir grassland white mushroom, Hailar white mushroom, Hailar white mushroom, belongs to Basidiomycotina, Lamimycetes, non-separated Basidiomycetes, Agaricaceae, White Mushrooms, and White Mushrooms. It is a large wild edible fungus. [0003] The cap of the prairie white mushroom is hemispherical to flat, white and smooth, the toadstool is thick and tender, the stipe is middle-grown, strong and solid, the base is slightly larger, the spore print is white, the mycelium is snow-white, dense and strong, without inversion hairy phenomenon, and the fruiting body mushroom has a strong aroma. [0004] Grassland white mushroom is a precious edible fungus that has attracted widespread att...

Claims

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Application Information

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IPC IPC(8): A01G1/04
CPCY02A40/20
Inventor 方在吉
Owner 方在吉
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