Artificial cultivation method of hair weeds

A cultivation method and artificial cultivation technology, applied in the fields of botanical equipment and methods, horticulture, unicellular algae, etc., can solve the problems of consuming dry matter of algae, unable to grow algae, etc.

Inactive Publication Date: 2003-12-10
SHANTOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the original plant is soaked in water, there is no net photosynthetic carbon accumulation. On the contrary, due to the enhanced respiration, the respiration exceeds the photosynthetic production, which may consume part of the original dry matter of the algae, resulting in the inability of the algae to grow.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Prepare liquid medium 1, 2, solid medium and solid medium plate according to the following formula

[0023] Liquid medium 1 is composed of the following components in proportion: sodium nitrate 100mg3H 2 ODipotassium hydrogen phosphate 5.3mg7H 2 O magnesium sulfate 7.8mg calcium chloride 2.8mg9H 2O sodium silicate 6mg citric acid 0.6mg ferric ammonium citrate 0.6mg hexamethylenediaminetetraacetic acid 0.1mg sodium carbonate 2mg trace metal solution 0.1ml distilled water 99.9ml

[0024] The trace metal solution in the liquid medium 1 is composed of the following components in proportion: boric acid 286mg4H 2 O manganese chloride 183mg1H 2 O manganese sulfate 153mg7H 2 O zinc sulfate 22.5mg5H 2 O copper sulfate 7.6mg sodium molybdate 2.5mg distilled water 100ml

[0025] Liquid medium 2 is composed of the following components in proportion: sodium nitrate 49.6mg dipotassium hydrogen phosphate 4mg7H 2 O magnesium sulfate 7.7mg2H 2 Calcium O chloride 3.8mgH 2 O sodi...

Embodiment 2

[0040] The preparation method of liquid medium 1, liquid medium 2, solid medium and solid medium plate is the same as that of Example 1.

[0041] The steps of the artificial cultivation method of Nostocs seedlings are as follows:

[0042] a. Select 0.5g of the original plant of Nostocchia radiata with thicker filaments and brighter color, remove the weeds, and then wash away the impurities inside with clean water, which will be used in the next step to separate the cells;

[0043] Wash the surface of the screened original plant with distilled water several times on the ultra-clean workbench, then soak it in 60% ethanol for 58 seconds, then rinse it repeatedly with sterile water for 7 times, and set aside;

[0044] b. Put the washed hair vegetable protoplant at 10°C, 30μmolm -2 the s -1 Lighting, photosynthesis recovery under simulated natural light conditions, the recovery time is 12 hours, and the medium used is liquid medium 1;

[0045] c. Gently crush the Nostocella prot...

Embodiment 3

[0051] The preparation method of liquid medium 1, liquid medium 2, solid medium and solid medium plate is the same as that of Example 1.

[0052] The steps of the artificial cultivation method of Nostocs seedlings are as follows:

[0053] a. Select 0.5 grams of the original plant of Nostocchia radiata with thicker filaments and brighter color, remove the weeds, and then wash away the impurities inside with clean water, which will be used in the next step to separate the cells;

[0054] Rinse the surface of the screened original plant with distilled water several times on the ultra-clean workbench, then soak it in 88% ethanol for 12 seconds, then rinse it repeatedly with sterile water 10 times, and set aside;

[0055] b. Put the washed hair vegetable protoplant at 22℃, 30μmolm -2 the s -1 Under light conditions, simulate natural light for photosynthesis recovery, the recovery time is 8 hours, and the medium used is liquid medium 1;

[0056] c. Gently crush the Nostocella pro...

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Abstract

The artificial culture of hair weeds seedlings includes the following steps: selecting thallus of hain weeds, cleaning with distilled water, moistening with ethyl alcohol, repeatedly washing with sterile water, placing the cleaned thallus of hair weeds in the liquid culture medium to make photosynthesis and restoration, pulverizing the thallus of the hair weeds so as to obtain free unicell and filament, cultivating the unicell and filament in liquid culture medium to obtain the filament with thickened sheath, taking out the filament and transferring it on the surface of agar culture plates, then quantitatively watering the surface of culture medium at regular time every day.

Description

Technical field [0001] The invention relates to a method for artificially cultivating Nostocs seedlings. Background technique [0002] Nostoc flagelliforme (Nostoc flagelliforme) belongs to terrestrial cyanobacteria, mainly distributed in the arid and semi-arid areas of northern and northwestern my country, and is a precious edible and medicinal resource. It has rich nutritional value, rich in pectin, protein content 20-23.4%, carbohydrate 57%, fat less than 0.3%, phosphorus 0.063-0.211%, iron 0.07-0.20%, calcium 1.38-2.56%, ash content 4.87-5.57%, containing more than 20 kinds of trace elements such as zinc, manganese, copper, molybdenum, cobalt, iodine, etc., and 19 kinds of amino acids, the total amount of which is 17.79%, including 8 kinds of amino acids necessary for the human body, accounting for 10% of the total amino acid content 35.8-38.6%, also contains vitamin C, B1, B2 and so on. In China, the consumption of Fa Cai has a long history. As a precious edible spiru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C12N1/12
Inventor 高坤山叶长鹏
Owner SHANTOU UNIV
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