Cultivation and domestication method of wild collybia albuminosa
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A technology for the cultivation and domestication of wild gallicariae fungus and the original seed culture medium can solve the problems such as the increase of the cultivation cost and the occurrence of ant damage, and achieve the effects of fast fruiting and stable output.
Inactive Publication Date: 2016-04-13
ZHENJIANG SHENGHONG LANDSCAPE PLANT
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Under the natural conditions, the symbiotic life of the Gallus fir fungus and the termite camp, therefore, the existing research and development direction of the artificial cultivation of the albumen fungus is mainly to carry out the artif
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[0016] Example 1,
[0017] A method for cultivating and domesticating wild Corydalis, including the following steps:
[0018] S1. Separation of female seed: young somatic cells are directly extracted from the growing vegetative body of Corydalis cylindrica to induce callus, gradually cultivated into mycelium, and white and strong mycelium is selected as the mother seed;
[0019] S2. The configuration of the mother seed culture medium: 50% PDA+50% DDS, the PDA is 200g potato, 20g glucose, 20g agar, and 1000ml water;
[0020] S3. Inoculate the vigorous hyphae of the mother seed after propagation into the mother seed culture medium, cultivate in a constant temperature incubator at 25°C until the hyphae become full of the medium, and select robust hyphae as the original seed;
[0021] S4. The configuration of the stock culture medium: sawdust 25%, DDGS 45%, bran 20%, termite discharge 10%, and water 120%.
[0022] S5. Inoculate the original seed in a cultivation bottle containing the origina...
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[0026] Example 2,
[0027] A method for cultivating and domesticating wild Corydalis, including the following steps:
[0028] S1. Separation of female seed: young somatic cells are directly extracted from the growing vegetative body of Corydalis cylindrica to induce callus, gradually cultivated into mycelium, and white and strong mycelium is selected as the mother seed;
[0029] S2. The configuration of mother seed culture medium: 50% modified PDA+50% DDS, the modified PDA is 200g potato, 20g glucose, 20g agar, 1000ml water, 1g potassium hydrogen phosphate, 0.5g magnesium sulfate, and 2g yeast extract.
[0030] S4. The configuration of the original seed culture medium: 25% sawdust, 45% DDGS, 20% bran, 10% termite discharge, and 120% water;
[0031] S5. Inoculate the original seed in a cultivation bottle containing the original seed culture medium, and cultivate it in a constant temperature incubator at 25°C to obtain the cultivated seed;
[0032] S6. Cultivation culture medium configurat...
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Abstract
The invention relates to a cultivation and domestication method of wild collybia albuminosa. The method mainly comprises the following steps that 1, mother strain separation is conducted, delicate body cell induced callus tissue is directly extracted from a growing collybia albuminosa nutrition body, hyphae are cultivated gradually, and pure white and strong hypha bodies are selected to serve as mother strains; 2, preparation of mother strain culture media is conducted; 3, the hyphae of which the growth vigor is healthy and strong after expanding propagation is conducted on the main strains are inoculated into the mother strain culture media, culture is conducted in a constant temperature incubator with the temperature of 25 DEG C till the hyphae are overgrown in the culture media, and strong hyphae are selected to serve as original strains; 4, preparation of original strain culture media is conducted; 5, the original strains are inoculated into a cultivation bottle containing the original stain culture media, culture is conducted in the constant temperature incubator with the temperature of 25 DEG C, and cultivated species are obtained. According to the cultivation and domestication method of the wild collybia albuminosa, artificial cultivation of the collybia albuminosa on the condition that termites do not exist can be achieved, fruiting is fast, and the yield is stable.
Description
technical field [0001] The invention relates to the field of artificial domestication of wild fungi, in particular to a method for cultivation and domestication of wild colpodium fir fungus. Background technique [0002] Gallium fir fungus is a kind of edible fungus with rich nutritional value and delicious taste. It is the king of wild edible fungi. It is commonly found on the ground in coniferous and broad-leaved forests, on wasteland, in random graves, and in valleys. The base stalk is connected to the nest of termites (Odontotermessp.) , Scattered to group students. Under the natural conditions, the symbiotic life of the Gallus fir fungus and the termite camp, therefore, the existing research and development direction of the artificial cultivation of the albumen fungus is mainly to carry out the artificial cultivation of the albumen fungus and the artificial breeding of the termites at the same time, which not only causes the cultivation cost to be greatly increased, but...
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