Tissue culture method of rosa graciliflora

A technique of tissue culture and rose, which is applied in the field of plant cultivation and plant tissue culture, can solve the problems of few reports of wild rose seed germination, no seed germination and tissue culture research reports, etc., to achieve a large number of rapid propagation and good application Foreground, effect of high seed emergence

Inactive Publication Date: 2020-07-28
JALA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few reports on the germination of wild rose seeds. Rose slender stems are completely a wild species, and there are no related research reports on seed germination and tissue culture.

Method used

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  • Tissue culture method of rosa graciliflora
  • Tissue culture method of rosa graciliflora
  • Tissue culture method of rosa graciliflora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 Seed Germination Treatment

[0038] wild rosebush flowers figure 1 Shown in A, in November, ripe thin stem rose fruit (such as figure 1 B) pick it off, cut out the seeds in time and clean them up, and dry them for later use (such as figure 1 C), then mix the seeds and wet sand evenly in a volume ratio of 1:5, keep the humidity at 70% to 80%, and perform low-temperature sand storage (low-temperature stratification) treatment at 5°C. In September of the following year, the seeds stored in the sand were taken out for sowing. The substrate was peat: perlite = 3:1 (volume ratio), the pot soil was kept moist, and normal cultivation and management were carried out after emergence. 1 month after sowing, the seedling emergence rate was 35% (such as figure 1 D).

[0039] Seed germination rates of different low-temperature sand storage time treatments are shown in Table 1.

[0040] Table 1 Effect of different low temperature stratification time on seed germinatio...

Embodiment 2

[0043]Embodiment 2 stem segment tissue culture

[0044] (1) Disinfection of explants

[0045] Processed according to the method of embodiment 1, after germination and seedling growth one year (cultivated plants such as figure 2 A'), get the well-grown annual stem section of Rosa spp., remove the leaves and petioles, and rinse under running water for 30 minutes. Cut off a 2-3cm stem section containing a nodule (stem node), soak and disinfect with 70% ethanol for 30s, rinse with sterile water for 3 to 4 times; then use 10% sodium hypochlorite containing about 0.1% (wt) Tween 20 The solution (available chlorine is about 1%) is disinfected for 10 minutes, rinsed with sterile water for 3 to 4 times; sterilized with 0.05% mercuric chloride for 5 minutes, and rinsed with sterile water for 3 to 4 times.

[0046] (2) Axillary bud induction

[0047] Inoculate the sterilized thin-stemmed rose stems vertically upward into the primary medium, 5 stems per bottle, and put them in an incu...

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Abstract

The invention relates to plant tissue culture, and discloses a tissue culture method of rosa graciliflora. The tissue culture method comprises the following steps: (1) axillary bud induction: taking an annual stem section with a stem node of the rosa graciliflora as an explant, and inoculating the explant into a primary culture medium for axillary bud induction culture; (2) cluster bud induction and proliferation: transplanting a germinated axillary bud into a proliferation culture medium for cluster bud differentiation and proliferation; and (3) rooting culture: transferring a seedling obtained in the step (2) into a rooting culture medium, and performing rooting culture for 14-30 days. According to the culture method disclosed by the invention, artificial culture of the rosa graciliflorais realized for the first time, the seed emergence rate, the axillary bud germination rate, the proliferation coefficient and the rooting rate are high, massive rapid propagation of the rosa graciliflora is facilitated, and the tissue culture method has good application prospects.

Description

technical field [0001] The invention relates to a method for plant cultivation and plant tissue culture, in particular to a method for obtaining regenerated plants through tissue culture with stem segments of rose stalks as explants. Background technique [0002] Rosa L. is one of the world-renowned ornamental plants and is widely cultivated in gardens. There are more than 200 species in the world, of which about 95 species are native to China, which is one of the important distribution centers of Rosa resources. Thin stem rose (Rosagraciliflora Rehd.et Wils), also known as thorn chestnut, mostly grows on hillsides, under spruce forests or forest edge shrubs at an altitude of 3300-4500 meters, only in parts of Yunnan, Sichuan, Tibet and Shaanxi distributed. [0003] Thin-stemmed rose has bright colors and pleasant fragrance. It is an excellent landscaping material. In addition, its roots and fruits can be used as medicine, which can not only improve immunity, but also play...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 李惠玲章漳
Owner JALA GROUP CORPORATION
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