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Culture medium used for batrachospermaceae and application of culture medium

A kind of culture medium, the technology of the algae, applied in the medium field of the freshwater red alga Conchrys spp.

Inactive Publication Date: 2016-05-04
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the laboratory culture of Monocele, and its research is limited to collecting samples in the field, fixing them with formalin solution for morphological observation, or using silica gel to dry and preserve them for molecular biology research

Method used

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  • Culture medium used for batrachospermaceae and application of culture medium
  • Culture medium used for batrachospermaceae and application of culture medium
  • Culture medium used for batrachospermaceae and application of culture medium

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Using culture medium of the present invention to carry out laboratory culture to Monocerula arcus

[0030] Carry out artificial cultivation to the Trichomonas genus Trichomonas collected in the field, the preparation and culture method of culture medium are as follows:

[0031] The steps for preparing 1L Monococcus culture medium are as follows:

[0032] Medium composition ratio:

[0033] NaNO 3 2.92mM, K 2 HPO 4 0.42mM, KH 2 PO 4 1.28mM, MgSO 4 .7H 2 O0.29mM, CaCl 2 0.16mM, NaCl 0.43mM, PIV metal solution 6.0mL / L, vitamin B12 solution 0.9mL / L, vitamin H solution 0.9mL / L, vitamin B1 solution 0.9mL / L, penicillin-streptomycin solution 0.008mL / L.

[0034] Components and content thereof of the described PIV metal solution: Na 2 EDTA.2H 2 O2.0mM, FeCl 3 .6H 2 O0.36mM, MnCl 2 .4H 2 O0.20mM, ZnCl 2 0.036mM, CoCl 2 .6H 2 O0.0083mM, Na 2 MoO 4 .2H 2 0.016mM.

[0035] Described vitamin B12 solution is made by following method: in 200mLdH 2 Add 2.40 g of HEP...

Embodiment 2

[0054] Carry out artificial culture to the Trichomonas genus Micella zhongjiao collected in the field, culture medium preparation and culture method are the same as example 1, concrete implementation result is as follows Figure 4 , 5 and 6.

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Abstract

The invention provides a culture medium used for batrachospermaceae and application of the culture medium. The culture medium used for the batrachospermaceae is mainly prepared from the main components including NaNO3, K2HPO4, KH2PO4, MgSO4.7H2O, CaCl2, NaCl, a PIV metal solution, a vitamin B12 solution, a vitamin H solution, a vitamin B1 solution, a penicillin-streptomycin solution and distilled water. By utilizing the culture medium, fresh batrachospermaceae algae collected in the wild are artificially cultured in a laboratory, and an indoor living state of the batrachospermaceae is kept. With the adoption of the culture medium provided by the invention, the difficulties that previous batrachospermaceae researches are only limited to wild collection and growth and the batrachospermaceae cannot be artificially cultured, so that subsequent life history observation, molecular biological analysis can not be performed and the like are overcome.

Description

technical field [0001] The invention relates to an algae culture medium, in particular to a culture medium of freshwater red algae Monococcus and application thereof. Background technique [0002] Monocele is a typical group of freshwater red algae with harsh habitat conditions. It usually grows sessilely in cool and clean water. The sessile substrate is sand, river bed or other plants, and only in relatively stable environment with little change in environmental factors. It can only grow in water bodies, such as cold springs, well water, streams, etc., in a point-like distribution. The adaptability of Monocele to the environment is very fragile, resulting in the rare distribution of this group. At present, there is no report on the laboratory culture of Monocele, and its research is limited to collecting samples in the field, fixing them with formalin solution for morphological observation, or using silica gel for molecular biology research. Contents of the invention ...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 南芳茹谢树莲冯佳吕俊平刘琪
Owner SHANXI UNIV
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