76 results about "Double probe" patented technology

The invention relates to the technical field of integrated circuit testing, in particular to a distance-adjustable probe connector device which comprises an impedance detection unit which comprises a connector fixing part, a connector rotating part, a single connection port, a signal probe and a grounding probe. The impedance data unit is used for determining a characteristic impedance value of the tested microstrip line based on data acquired by the signal probe and the grounding probe; the pressure detection unit is used for determining the contact pressure according to the telescopic displacement of the signal probe and the grounding probe; the control unit determines the allowable range of the contact pressure of the probes based on the line width of the microstrip line to be tested and the distance between the probes, and adjusts the angle between the grounding probe and the surface of the PCB based on the contact pressure difference between the signal probe and the grounding probe and the line width of the microstrip line. And adjusting the contact pressure of the signal probe to obtain the effective characteristic impedance value of the tested microstrip line. According to the invention, the angle and pressure of the grounding probe are intelligently adjusted, and the problem of impedance measurement drift caused by pressure imbalance of double probes is eliminated.

The invention relates to a double-probe detection reagent for detecting salmonella typhimurium and a preparation method of the double-probe detection reagent, and belongs to the technical field of food safety. The double-probe detection reagent is formed by respectively packaging a magnetic separation probe reagent solution and a fluorescent nano-enzyme probe reagent solution; the magnetic separation probe reagent liquid is used for specifically binding salmonella typhimurium and carrying out separation and enrichment treatment on a detection sample; the red fluorescent nano-enzyme probe reagent solution is used for specifically combining the salmonella typhimurium subjected to separation and enrichment treatment and generating a salmonella typhimurium concentration dependent colorimetric signal and a fluorescent signal. The double-probe detection reagent disclosed by the invention is short in detection time and high in detection sensitivity when being used for detecting salmonella typhimurium, and has relatively good specificity on common type strains. According to the invention, the specific surface area is high, the peroxidase-like activity is excellent, and the high-sensitivity capture efficiency is enhanced; according to the invention, mutual verification is carried out between the dual-mode signals, an internal proofreading effect is achieved, and the detection result is accurate.

The invention relates to an excess feed detection system, an intelligent porridge machine and an intelligent feeding system and method.The excess feed detection system comprises a probe module and an ultrasonic module which are arranged in the intelligent porridge machine; the probe module comprises two groups of probes, and the two groups of probes are respectively connected with a body controller in the intelligent porridge machine through wires; when the two groups of probes are in contact with porridge in the bowl, a detection loop formed by the body controller and the two groups of probes is conducted; the ultrasonic module comprises two groups of ultrasonic sensors, and the two groups of ultrasonic sensors are symmetrically arranged on the left side and the right side of the intelligent porridge machine. The excess feed detection system solves the problem of failure of dry feed detection of traditional double probes, overcomes the defect that single ultrasonic detection is easy to pollute and block, not only improves the detection precision, but also is beneficial to ensuring healthy growth of pigs.

The invention provides a de-embedding-based optoelectronic chip bandwidth test method and system, and the method comprises the steps: employing a double-probe frequency response characteristic separation technology, firstly building a mathematical model comprising a radio frequency probe frequency response matrix and an optical probe frequency response matrix, respectively extracting probe characteristic parameters through a multi-stage calibration process, and carrying out the multi-stage calibration process; the method comprises the following steps of: firstly, deducting probe influence from original test data by adopting matrix vector operation, retaining chip response and finally realizing accurate bandwidth calculation, eliminating the influence of impedance mismatch on a test result by de-embedding a radio frequency probe and an optical probe, improving the linearity of a frequency response curve, enabling the test result to be smoother, reducing noise and improving the test accuracy. And meanwhile, the smoothness and the repeatability of a test curve are improved, and a reliable test means is provided for research and development and quality control of a high-speed photoelectric device.

The present invention relates to a dual-probe detection reagent and a preparation method for detecting Salmonella typhimurium, and belongs to the field of food safety technology. The dual-probe detection reagent is composed of a magnetic separation probe reagent solution and a fluorescent nanozyme probe reagent solution, which are packaged separately; the magnetic separation probe reagent solution is used to specifically bind to Salmonella typhimurium and separate and enrich the test sample; the red fluorescent nanozyme probe reagent solution is used to specifically bind to the Salmonella typhimurium after separation and enrichment, and generate a Salmonella typhimurium concentration-dependent colorimetric signal and a fluorescent signal. The dual-probe detection reagent of the present invention has a short detection time and high detection sensitivity for the detection of Salmonella typhimurium, and has good specificity for common model strains. The present invention has a high specific surface area and excellent peroxidase-like activity, which enhances the high-sensitivity capture efficiency; the dual-modal signals of the present invention verify each other, play an internal proofreading role, and the detection results are accurate.

The invention belongs to the field of single cell level nucleic acid detection, and particularly relates to a construction method and application of a high-throughput single cell transcriptome library based on probe targeted enrichment. According to the method, single cell fixed punching and hybridization enrichment of a specific targeting double probe designed by a target nucleic acid sequence are combined, the nucleic acid sequence comprises mRNA and non-coding RNA, the probe is connected with beads with barcodes through a connecting sequence on the probe, cell labeling is carried out, and library building steps such as extension and amplification are completed; therefore, the problems of insufficient transcriptome integrity, single detection target and low number of detected genes are solved.

The invention discloses a double-detector linkage automatic fire extinguishing control method and system for an outdoor charging place, and belongs to the technical field of fire safety. The system comprises a water source supply unit, a detection alarm unit, a control execution unit and a fire extinguishing spraying unit. A dual detection mechanism of the temperature sensing detector and the flame detector is adopted, only when the two detectors give an alarm at the same time, the input and output module judges that a fire behavior is real, and signals are synchronously distributed to the automatic control switch, the fire control room and the charging control cabinet. The system provides an automatic, remote and manual three-start mode to ensure the reliability; the charging control cabinet automatically cuts off a power supply to prevent electric shock after receiving the signal; the anti-freezing design that an underground cement pool is combined with an overflow valve emptying pipeline is adopted, and normal operation can be achieved in the environment of minus 35 DEG C. According to the invention, the risk of false alarm of a single detector is thoroughly eliminated, the response time does not exceed 30 seconds, and the technical problems of low fire detection accuracy, poor winter adaptability and lack of power linkage protection in an outdoor charging place are effectively solved.

The invention relates to the technical field of semiconductor probe performance testing, in particular to a probe performance testing method, which comprises the following steps of: calculating resistance consistency and a signal transmission fidelity ratio of a probe interference working condition through a contact simulation test and a signal transmission simulation test, and performing single-probe simulation verification; for double probes formed by qualified single probes, calculating contact pressure interference variables and electromagnetic crosstalk interference variables, and carrying out cooperative interference judgment; extracting historical probe false data, and constructing an interference false risk model and a non-interference false risk model; grouping the double probes according to interference degrees, and substituting the grouped double probes into corresponding models to calculate risk assessment values; determining an adjustment direction and an adjustment scheme for the high-risk group, and calculating the average attenuation rate of the double probes after adjustment; and after the attenuation rate is verified to reach the standard, the probe group is determined as a qualified performance test probe group, so that the accuracy, the stability and the anti-interference capability of semiconductor probe performance detection are effectively improved.

This invention discloses a microRNA-responsive 1,2-dioxane-based chemiluminescent compound, its dual-probe system, kit, synthesis method, and applications. The compound's core is an o-acrylate-substituted phenoxyadamantane-1,2-dioxane, with the phenolic hydroxyl group protected by an azide benzyl group. The two-component probe system constructed based on this compound comprises an azide-modified first probe and a phosphine-modified second probe, each complementary to different segments of the target microRNA. Hybridization brings the two probes closer, triggering a Staudinger reaction to remove the protecting group and activate the chemiluminescent signal. This probe system requires no enzyme amplification or nucleic acid extraction, achieves a detection limit of 1.37 fM, and a signal-to-noise ratio as high as 157-fold. It can be successfully used for real-time imaging of endogenous microRNA-21 in live cells, showing broad application prospects in early tumor screening, auxiliary diagnosis, and prognostic assessment.

The invention discloses a switching system and method for realizing multi-probe random switching of multiple power supplies, and the system comprises an upper computer, a detection unit, an isolation unit, and a plurality of power supply single-double switching units, and the upper computer is disposed at a remote end; and the plurality of power supply single-double switching units are used for switching signals according to the isolated single-double probe modes and realizing any combination connection between the plurality of groups of power supplies and the plurality of probes, and each group of power supplies is connected with two probe switching circuits through a first relay, so that one power supply drives the two probes to work in a single-probe mode or a double-probe mode. According to the invention, a plurality of power supplies can be switched to be directly paired with a plurality of probes at will through remote control, and the power supplies can work in a single-probe mode or a double-probe mode without manual switching. The operability of random switching between a plurality of power supplies and a plurality of probes is greatly improved, meanwhile, the problem that on-site switching cannot be achieved manually is solved, and the state and the service life times of a relay can be monitored.

The invention discloses a steel rail welding seam ultrasonic flaw detection method and device. The method comprises a TOFD detection method, a single-probe reflection detection method and a double-probe K-type detection method. A plurality of pairs of rail side flaw detection probes and different combinations of zero-degree rail surface probes and inclined rail surface probes are arranged on two sides of the inner and outer side surfaces of a steel rail from bottom to top by taking a welding seam as a center, and a TOFD detection method, a single-probe reflection detection method and a double-probe K-type detection method are adopted, so that all-directional and multi-angle detection of a steel rail welding seam area is realized. By means of the method, longitudinal defects, transverse defects and defects at different depth positions in the welding seam can be effectively detected, the leakage detection risk is greatly reduced, and the comprehensiveness and reliability of steel rail welding seam quality detection are ensured.

The application discloses a Ka-band eight-way power combiner, which comprises a shielding box body, wherein a straight-through input port, an input waveguide cavity, an input coplanar magic T, a secondary waveguide power division module, a waveguide-microstrip double-probe conversion structure, a secondary waveguide power synthesis module, an output waveguide cavity, an output coplanar magic T and a straight-through output port are sequentially arranged in the shielding box body along a signal transmission direction; the secondary waveguide power division module and the secondary waveguide power synthesis module both adopt an E-T junction structure and are integrated with a three-stage waveguide E-plane stepped structure, so as to realize impedance matching from an input end to an output end; the Ka-band eight-way power combiner has high synthesis efficiency, extremely low amplitude imbalance and phase imbalance in the Ka band through multi-stage waveguide impedance matching, optimized double-probe conversion and symmetrical layout design. The combiner has the advantages of compact structure, large power capacity, simple machining and assembly and good stability.

The invention provides a phased array ultrasonic detection device and an automatic scanning system suitable for a circumferential weld of a small-diameter pipe. The automatic scanning system comprises a scanning frame, a path monitoring module, a coupling device, a driving device and a control device, the scanning frame comprises a cross rod and two probe clamping mechanisms arranged on the cross rod; the path monitoring module comprises an industrial camera, and the industrial camera is arranged on the cross rod, can perform angle adjustment and is used for monitoring a scanning path in real time; the coupling device can convey a coupling agent to the probe; the driving device is connected with the scanning frame and can walk; the control device can control walking of the driving device. The detection device comprises the automatic scanning system and a phased array ultrasonic detection instrument. According to the invention, while the requirements of stable coupling and constant-speed scanning are met, the double probes are symmetrically placed on the two sides of the welding seam for simultaneous scanning, and the scanning path is monitored in real time and the scanning track is remotely controlled and corrected by virtue of the industrial camera, so that the scanning precision is ensured.

The invention discloses a high-temperature and high-pressure integrated cable formation tester. The technical problems that an existing cable formation tester is poor in temperature-resistant and pressure-resistant effect, low in integration level, single in function and the like are solved. The cable formation tester comprises an electronic circuit pup joint, a multi-PVT sampling pup joint, a fluid identification pup joint, a pumping pup joint, an upper jam releasing pup joint, a double-probe pup joint, a lower jam releasing pup joint, a 3D probe pup joint and a double-packer pup joint which are sequentially connected from top to bottom. The fluid identification short section is provided with a density probe, a conductivity probe, a viscosity probe, a pressure probe, a temperature probe, a spectral component probe and a spectral fluorescence probe, and the density probe, the conductivity probe, the viscosity probe, the pressure probe, the temperature probe, the spectral component probe and the spectral fluorescence probe are integrally packaged on the fluid identification short section. The cable formation tester can meet the continuous working condition in the high-temperature and high-pressure environment, and is higher in integration level, more comprehensive in function and more reliable in operation.

The application relates to the technical field of biological detection, in particular to a method for in-situ quantitative detection of specific glycosylation of target protein based on double probes. The method provided by the application comprises the following steps: a protein probe and a glycan probe are used to combine with target protein and target glycan respectively, and the spatial proximity relationship after combination triggers hybridization between the probes on the same target protein to form hybrid double strands; the DNA enzyme of the protein probe is used to enzymatically cut the glycan probe on the hybrid double strands under the action of a metal cofactor; and the report peptide released after enzymatic cutting of the glycan probe is subjected to mass spectrometric quantitative analysis, so that the signal of protein-specific glycoform is converted into a mass spectrometric signal.

The invention relates to a double-probe detection kit for detecting staphylococcus aureus in meat products and a preparation method of the double-probe detection kit, and belongs to the technical field of food safety. The double-probe detection kit is composed of a magnetic capture probe reagent and a double-signal nano-enzyme probe reagent which are independently packaged and have the same volume; the magnetic capture probe reagent is a vancomycin modified reticular magnetic bead and is used for specifically binding staphylococcus aureus in a meat product detection sample and carrying out separation and enrichment treatment; the double-signal nano-enzyme probe reagent is a blue fluorescent carbon dot modified by a nucleic acid aptamer and is used for specifically combining the staphylococcus aureus subjected to separation and enrichment treatment and generating a staphylococcus aureus concentration dependent colorimetric signal and a fluorescent signal. According to the invention, mutual verification is carried out between dual-mode signals, so that the accuracy of a detection result is improved; according to the method, the detection time is within 1.5 h, the detection time is short, the detection sensitivity is high, and the method has good specificity for common type strains.

The application discloses a kind of thermal desorption treatment contaminated soil thermal conductivity test system and test method, test system includes test cavity, heating furnace, gas pressure control system and soil sample thermal conductivity test system, gas pressure control system includes gas pressure sensor, gas pressure control valve, nitrogen source, tail gas processor and vacuum pump, soil sample thermal conductivity test system includes double probe, wire, direct current power supply, multichannel temperature recorder and thermal conductivity calculation system, the double probe insertion to be measured soil sample and place in test cavity, the application can operate under high temperature environment, simultaneously, two different test methods are used to calculate two thermal conductivities for the same to-be-tested soil sample respectively, and the mean value is obtained by comparison and verification, effectively save the time of parallel test under high temperature environment, reduce the experimental error of calculating thermal conductivity.

The invention provides an automatic test method, device and equipment and a storage medium, the automatic test method is applied to the automatic test equipment, the automatic test equipment comprises a driving mechanism, a detection mechanism and a double-probe mechanism, and the automatic test method comprises the following steps: controlling the driving mechanism to drive probes in the double-probe mechanism to move to a detection point position of a to-be-detected material; when the probe is in contact with the detection point, collecting measurement data of the detection point through a detection mechanism; judging whether the to-be-detected material is an unqualified material or not according to the measurement data and preset standard information; and when the to-be-detected material is an unqualified material, obtaining abnormal data in the measurement data. According to the invention, the automatic measurement of the measurement data of the to-be-detected material can be realized, the measurement precision is improved, the labor cost is saved, the unqualified material can be detected automatically, rapidly and accurately, the yield is improved, the abnormal data of the unqualified material can be obtained automatically, and the detection efficiency is improved. The automatic analysis of the disqualification reason of the to-be-detected material is realized.

The invention belongs to the field of chemical detection, and relates to a preparation method and application of a CdSe / ZnS quantum dot labeled double-probe fluorescence immunochromatography test strip. The test strip comprises a bottom plate, a nitrocellulose membrane, a sample pad, a conjugate pad and a water absorption pad, the nitrocellulose membrane is located above the bottom plate, the sample pad and the conjugate pad are sequentially arranged on the bottom plate on the left side of the nitrocellulose membrane, and the water absorption pad is arranged on the bottom plate on the right side of the nitrocellulose membrane; quantum dots with two wavelengths are distributed on the conjugate pad and are respectively marked with two probes of mouse antibodies of two pesticides, and a thiamethoxam antigen, a clothianidin antigen and a goat anti-mouse secondary antibody strip are further distributed on the nitrocellulose membrane. The invention further provides a visual and rapid detection test strip suitable for quantitatively detecting the residual quantity of thiamethoxam and clothianidin in spinach, the cross reaction is lower than 10% or below of the rapid detection requirement, and the visual and rapid detection test strip has market application and popularization value.

The utility model belongs to the technical field of monitoring system maintenance, and relates to an auxiliary device based on monitoring system maintenance, which comprises a mounting plate, a universal meter body and exploring tubes, the two exploring tubes are respectively positioned on the outer side of the mounting plate, the mounting plate is U-shaped, the universal meter body is arranged in the mounting plate, the bottom of the mounting plate is provided with a position fixing mechanism, and the position fixing mechanism is fixed on the universal meter body. The universal meter body is symmetrically provided with the connecting lines, one end of each connecting line is connected with the probe tube, and the universal meter body can be fixed on the wrist or other places through the mutual cooperation of the mounting plate, the supporting plate, the cylindrical block, the first spring, the positioning plate, the magic tape bandage and the like. When the universal meter is used, the probe tube can be clamped on an object needing to be measured, in addition, the universal meter body can be separated from the mounting plate when needed, the universal meter is flexible to use, and the problems of unstable operation and data deviation easily caused by holding the universal meter with a single hand and controlling the double probes are effectively solved.

The application discloses a nucleic acid absolute quantification method based on nucleic acid constant temperature amplification, which comprises the following specific steps: step one, sample pretreatment, blood samples and saliva samples are pretreated respectively; step two, reaction system configuration and assembly; step three, digital micro-reaction unit preparation and amplification; and step four, multiple signal analysis and absolute quantification. In the application, multiple primer design and a double-probe system are adopted, so that the mutation detection rate is improved, and the detection limit can be reduced to 0.1% variation through Cas12a-sgRNA auxiliary verification. The double-emulsification technology of the micro-fluidic chip is adopted, so that the droplet diameter variation coefficient is controlled within 5%, and the clogged micropore rate is reduced by real-time analysis of the micropore array image through the ImageJ software, automatic marking and exclusion of abnormal units.

The present invention discloses a precise measurement device for complex columnar parts and its calibration method, belonging to the technical fields of intelligent manufacturing and precise measurement of columnar parts. The device consists of a motion drive and control unit, a composite clamping unit, a double probe unit and a frame. The composite clamping unit is designed with a replaceable clamping module and a new type of center, and has two clamping modes: double-center clamping and chuck-center clamping, which can meet the full-condition clamping of columnar parts. Secondly, the double probe unit can obtain the full-size information of the workpiece by using two line-structured light probes. And the motion drive and control unit adopts a differential gear train, and only a single motor is required to complete the clamping and measurement of the workpiece, further reducing costs and simplifying operations. In addition, the calibration method calibrates the probe pose in real time according to the combined features, ensuring the measurement accuracy. The present invention can achieve full-condition clamping and full-information measurement of complex columnar parts, with high precision and low cost, and has strong practicability and versatility.

1. The name of the design product: ultrasonic ranging sensor (double probe core). 2. The use of the design product: for detecting the distance between the object and the surface of the sensor. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The invention discloses a 5GC industrial private network switching delay detection method, which comprises the following steps of: setting timestamps of a control surface and a data surface more finely by using a double-probe measurement mechanism through a first probe and a second probe which are respectively arranged at an N2 interface and an N3 interface, and calibrating clock skew between the double probes in cooperation with a 5G synchronous clock, so that the accuracy of the switching delay of a 5GC industrial private network is improved. Multi-stage time delay is calculated by obtaining wireless transmission time delay, 5GC forwarding time delay and a difference value of processing starting and ending timestamps of each stage of a protocol stack composed of an IP, a UDP and a GTP-U, so that the network quality of equipment in operation is accurately calculated, the detection accuracy is improved, and by compiling keepalived supporting bidirectional forwarding detection and cooperating with main and standby node switching cooperation of a VRRP, the network quality of equipment in operation is improved. Millimeter-level fault detection is realized, the fault detection efficiency is improved, the network fault detection period is shortened, the response speed is improved, millisecond-level switching is realized, and the switching efficiency is improved.

The invention discloses equipment for testing the mechanical life of a contactor, and particularly relates to the technical field of direct current contactors, the equipment comprises a contactor testing machine and the contactor, the surface of a tool plate is provided with an installation mechanism for installing or replacing a test probe, and through the installation mechanism and an oil pressure buffer, the test probe is installed or replaced. The air cylinder is started to control the tool plate to move downwards, one end of the probe is made to make contact with the contactor, the position arrangement of the probe is related to whether all installation point positions of the contactor to be detected are qualified or not, synchronous detection of multiple stations can be achieved according to the multiple sets of grooves, in the falling detection process, one end of the probe makes contact with the contactor, and the detection accuracy is improved. A sensor or an inductor on the probe is utilized, an alarm can be given when an object to be detected is detected to be unqualified, the error detection probability is reduced by using the double probes, the influence caused by vibration during testing is reduced by using the oil pressure buffer, and meanwhile, the probe is adopted for detection, so that a wiring harness connection mode is not needed, and the remodeling time is further shortened.

To provide a method for enrichment of at least one target nucleic acid in a library of nucleic acids.SOLUTION: A first oligonucleotide is hybridized to a target nucleic acid in library of nucleic acids having first and second adapters. The hybridized first oligonucleotide is extended with a first polymerase, thereby producing a first primer extension complex including the target nucleic acid and the extended first oligonucleotide. The first primer extension complex is captured, enriched relative to the library of nucleic acids, and a second oligonucleotide is hybridized to the target nucleic acid. The hybridized second oligonucleotide is extended with a second polymerase, thereby producing a second primer extension complex including the target nucleic acid and the extended second oligonucleotide, and further liberating the extended first oligonucleotide from the first primer extension complex.SELECTED DRAWING: Figure 1

The invention discloses a multi-probe imaging mechanism of full-ring SPECT-CT equipment. The multi-probe imaging mechanism comprises an annular mounting disc, a plurality of probes and a plurality of probe driving assemblies, the number of the probes and the number of the probe driving assemblies are both 3-9, and the probes and the probe driving assemblies are in one-to-one correspondence. The plurality of probes are uniformly distributed in the circumferential direction of the annular mounting disc, each probe is movably arranged on the annular mounting disc in the radial direction of the annular mounting disc through the corresponding probe driving assembly, and the probe driving assemblies are used for driving the probes to move. The problems that most of existing SPECT-CT devices are single-group or double-group probes and cannot fully cover organs of the whole body of the human body, the probes need to be frequently adjusted for one-by-one detection when different organs are detected, and the whole body detection efficiency is low are solved.

The invention discloses a probe for researching a working mechanism of NAD (Nicotinamide Adenine Dinucleotide) or a metabolite thereof and application of the probe. The probe is a double-probe combination and comprises a first probe and a second probe, the first probe is azide-modified nicotinamide adenine dinucleotide or azide-modified nicotinamide adenine dinucleotide metabolite, namely, an azide group is introduced to the adenine 8 site of the nicotinamide adenine dinucleotide or the nicotinamide adenine dinucleotide metabolite; enabling the azide group and adenine of nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotide metabolite to form a photo-crosslinking reaction group; and the second probe is an EBT probe with biotin. The molecular structure of the first probe is closer to that of the NAD or the metabolite of the NAD, the interaction protein captured by the first probe can better reflect the real binding condition of the NAD or the metabolite of the NAD and the interaction proteome, and therefore the working mechanism of the NAD or the metabolite of the NAD can be reflected more accurately and truly.

The present invention belongs to the field of optical engineering, and specifically relates to a method for adjusting the optical power symmetry of a cone assembly of a double-probe illuminated laser module, including the following steps: (1) designing a device for adjusting the symmetry of the laser module cone assembly; (2) collecting characteristic images at the bottom of the laser module; (3) designing a lighting scheme; (4) image processing; (5) target feature extraction; (6) image angle compensation; (7) adjusting the optical power symmetry; The present invention uses the method of energizing the double probes, completely energizing from the bottom of the laser module, so that the laser module with an insulated outer cylinder can be normally illuminated in the improved double-probe JAS-15 chuck; Four groups of optical power acquisition components clamp the directions of two strong lights and two weak lights, which can effectively improve the adjustment accuracy and shorten the adjustment time; The sobel operator is used to extract the target contour, and the mathematical morphology method is used to process the target contour, and the edge integrity of the extracted graphic target features is better.

The invention relates to a double-probe detection kit for detecting escherichia coli O157: H7 and a preparation method of the double-probe detection kit, and belongs to the technical field of food safety. The double-probe detection kit consists of a magnetic separation probe reagent and a copper-manganese double-metal nano-enzyme probe reagent which are independently packaged. The magnetic separation probe reagent is a concanavalin agglutinin A modified magnetic bead, and is used for specifically binding escherichia coli O157: H7 in a meat product detection sample and carrying out separation and enrichment treatment; the bimetallic nano-enzyme probe reagent is copper-manganese bimetallic nano-enzyme modified by a nucleic acid aptamer, and is used for specifically combining with the escherichia coli O157: H7 subjected to separation and enrichment treatment and generating an escherichia coli O157: H7 concentration dependent colorimetric signal and a fluorescence signal. According to the invention, mutual verification is carried out between dual-mode signals, so that the accuracy of a detection result is improved; the detection time is within 1.5 h, and the method has good specificity for common type strains. The invention provides a design thought for the development of detection sensors for escherichia coli O157: H7 and other meat-derived pathogenic bacteria.