High accuracy detection method of human papilloma virus genotype

Abstract

The invention provides a high accuracy detection method of human papilloma virus genotype. Double probe, which comprises a carcinogenicity early transcription region E6 or E7 gene region and a capsid protein late transcription region L1 or L2 gene region, is adopted to detect every genotype. According to the method, pollution exclusion of PCR amplification product is taken as a premise, identical genotype recognition by double gene probe is taken as a basis, and high sensitivity PCR-mass spectrum combination is taken as a platform. The invention provides a method for high accuracy detection and / or identification of human papilloma virus (HPV) genotype.

Description

technical field [0001] The invention relates to the field of detecting and identifying pathogenic microorganism preparations, in particular to a method for detecting human papilloma virus genotype with high accuracy. Background technique [0002] Dr. Harald zur Hausen in Germany confirmed in 1983 that certain types of human papillomavirus (HPV) are related to cervical cancer [1], and won the Nobel Prize in Physiology or Medicine in 2008. This achievement created a new direction for cervical cancer research, promoted the research and development of HPV genotype analysis and vaccines, and made cervical cancer the only human cancer with a clear etiology, preventable, early detection and cure. In 2003, Dr. Nubia Munoz of the International Cancer Research Institute published an analysis report by 11 research institutions in 9 countries, and according to the relationship between different types of HPV and cervical cancer, the detected 30 HPV genotypes were divided into three categ...

AI Technical Summary

Problems solved by technology

[0013] The invention provides a method for detecting the genotype of human papilloma virus with high precision, aiming at thoroughly solving the problem of false positives caused by the pollution of PCR amplification products that cannot be ruled out in existing methods; all detection methods use single gene probe For targeting, ignore the problem of false negatives caused by the deletion or mutation of the target fragment during the integration of the virus, especially the difference in the copy number of the E and L genes of the HPV virus between the latent and active phases; and the current detection methods have not reached the coverage 30 pathogenic HPV genotypes, false negative problems caused by missed detection

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