Synthetic adhering culture medium for cell culture and preparation method of culture medium

Abstract

The invention discloses a synthetic adhering culture medium for cell culture. Each liter of synthetic adhering culture medium is prepared from components as follows: 0.08 g of biotin, 20 mu g of corticosterone, 2 g of L-carnitine, 1.2 g of linoleic acid, 1.2 g of ethanolamine, 0.8 g of linolenic acid, 13-17 g of D-glucose, 5 mu g of progesterone, 1.2 g of sodium pyruvate, 0.08 g of retinyl acetate, 4 g of catalase, 0.9-1.2 g of folic acid, 0.8-1.3 g of reduced glutathione, 60-70 mu g of lipoic acid, 2-3 g of transferrin, 6-8 g of human insulin, 0.1 mg of sodium selenite, 500-600 mL of a banana peel extract, 1.2-2 g of naphthylacetic acid and 0.8-1.5 g of vitamin C. According to the synthetic adhering culture medium for cell culture and a preparation method of the culture medium, no animal serum is contained, the stem cell differentiation culture time is greatly shortened and the experiment cost is saved.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a synthetic adhesion medium for cell culture and a preparation method thereof. Background technique [0002] Animal cell culture, especially stem cell culture, has always been one of the basic experimental methods in the field of medical disease research. Stem cells can not only replicate and renew themselves, but also have the potential to differentiate into other types of cells. Therefore, they have good applications in the treatment of difficult diseases prospect. The stem cell culture process needs to rely on a special culture environment, and it is required to achieve the effect of stem cell adherence culture or proliferation. [0003] Since the culture of stem cells usually originates from the inoculation of a few cells, the initial inoculation amount is not much, so at present, the culture medium of stem cells mainly adopts the medium containing fetal bov...

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Problems solved by technology

[0003] Since the culture of stem cells usually originates from the inoculation of a few cells, the initial inoculation amount is not much, so at present, the culture medium of stem cells mainly adopts the medium containing fetal bovine serum and amino acids, and the stem cells are stimulated by fetal bovine serum to achieve The purpose of rapid division and differentiation of stem cells, however, the use of these cell culture medium to expand and differentiate stem cells requires a long process, often several weeks, and the extraction of fetal bovine serum requires complicated procedures and high experimental costs, so There is a need to develop a new medium to rapidly promote cell culture

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