Synthetic adhering culture medium for cell culture and preparation method of culture medium

A cell culture and culture medium technology, applied in cell culture active agents, biochemical equipment and methods, embryonic cells, etc., can solve the problems of high experimental cost, small initial inoculation amount, long time, etc., to shorten the culture time and save Experiment cost, effect of shortened time

Inactive Publication Date: 2017-09-01
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Since the culture of stem cells usually originates from the inoculation of a few cells, the initial inoculation amount is not much, so at present, the culture medium of stem cells mainly adopts the medium containing fetal bovine serum and amino acids, and the stem cells are stimulated by fetal bovine serum to achieve The purpose of rapid division and differentiation of stem cells, however, the use of these cell culture medium to expand and differentiate stem cells requires a long process, often several weeks, and the extraction of fetal bovine serum requires complicated procedures and high experimental costs, so There is a need to develop a new medium to rapidly promote cell culture

Method used

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  • Synthetic adhering culture medium for cell culture and preparation method of culture medium
  • Synthetic adhering culture medium for cell culture and preparation method of culture medium
  • Synthetic adhering culture medium for cell culture and preparation method of culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A synthetic adhesion medium for cell culture, each liter of synthetic adhesion medium contains the following components: biotin 0.08g, corticosterone 20μg, L-carnitine 2g, linoleic acid 1.2g, ethanolamine 1.2 g, linolenic acid 0.8g, D-glucose 15g, progesterone 5μg, sodium pyruvate 1.2g, retinyl acetate 0.08g, catalase 4g, folic acid 1g, reduced glutathione 1.0g, sulfur Caprylic acid 65μg, transferrin 2.5g, human insulin 7g, sodium selenite 0.1mg, banana peel extract 550mL, naphthaleneacetic acid 1.5g, vitamin C 1.2g, specifically prepared according to the following steps:

[0026] Step 1, Prepare Banana Peel Extract

[0027] Step 1.1, collecting fresh banana peels, roasting them at 115°C for 15 minutes to obtain carbonized banana peels;

[0028] Step 1.2, prepare the soaking solution according to the following formula, wherein each liter of the soaking solution contains the following components: 0.3-0.5g of vitamin B12, 1-2g of vitamin B1, and the balance is distilled ...

Embodiment 2

[0035] A synthetic adhesion medium for cell culture, each liter of synthetic adhesion medium contains the following components: biotin 0.08g, corticosterone 20μg, L-carnitine 2g, linoleic acid 1.2g, ethanolamine 1.2 g, linolenic acid 0.8g, D-glucose 15g, progesterone 5μg, sodium pyruvate 1.2g, retinyl acetate 0.08g, catalase 4g, folic acid 0.9g, reduced glutathione 0.8g, Lipoic acid 60μg, transferrin 2g, human insulin 6g, sodium selenite 0.1mg, banana peel extract 600mL, naphthaleneacetic acid 2g, vitamin C 1.5g. The preparation method is the same as in Example 1, except that the formula of the culture medium is changed to that of Example 2.

Embodiment 3

[0037] A synthetic adhesion medium for cell culture, each liter of synthetic adhesion medium contains the following components: biotin 0.08g, corticosterone 20μg, L-carnitine 2g, linoleic acid 1.2g, ethanolamine 1.2 g, linolenic acid 0.8g, D-glucose 15g, progesterone 5μg, sodium pyruvate 1.2g, retinyl acetate 0.08g, catalase 4g, folic acid 1.2g, reduced glutathione 1.3g, Lipoic acid 70μg, transferrin 3g, human insulin 8g, sodium selenite 0.1mg, banana peel extract 500mL, naphthaleneacetic acid 1.2g, vitamin C 0.8g. The preparation method is the same as in Example 1, except that the formula of the culture medium is changed to that of Example 3.

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Abstract

The invention discloses a synthetic adhering culture medium for cell culture. Each liter of synthetic adhering culture medium is prepared from components as follows: 0.08 g of biotin, 20 mu g of corticosterone, 2 g of L-carnitine, 1.2 g of linoleic acid, 1.2 g of ethanolamine, 0.8 g of linolenic acid, 13-17 g of D-glucose, 5 mu g of progesterone, 1.2 g of sodium pyruvate, 0.08 g of retinyl acetate, 4 g of catalase, 0.9-1.2 g of folic acid, 0.8-1.3 g of reduced glutathione, 60-70 mu g of lipoic acid, 2-3 g of transferrin, 6-8 g of human insulin, 0.1 mg of sodium selenite, 500-600 mL of a banana peel extract, 1.2-2 g of naphthylacetic acid and 0.8-1.5 g of vitamin C. According to the synthetic adhering culture medium for cell culture and a preparation method of the culture medium, no animal serum is contained, the stem cell differentiation culture time is greatly shortened and the experiment cost is saved.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a synthetic adhesion medium for cell culture and a preparation method thereof. Background technique [0002] Animal cell culture, especially stem cell culture, has always been one of the basic experimental methods in the field of medical disease research. Stem cells can not only replicate and renew themselves, but also have the potential to differentiate into other types of cells. Therefore, they have good applications in the treatment of difficult diseases prospect. The stem cell culture process needs to rely on a special culture environment, and it is required to achieve the effect of stem cell adherence culture or proliferation. [0003] Since the culture of stem cells usually originates from the inoculation of a few cells, the initial inoculation amount is not much, so at present, the culture medium of stem cells mainly adopts the medium containing fetal bov...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077C12N5/0775C12N5/0735
CPCC12N5/0606C12N5/0657C12N5/0663C12N2500/25C12N2500/30C12N2500/34C12N2500/36C12N2500/38C12N2500/44C12N2500/76C12N2501/39C12N2501/392C12N2501/71
Inventor 沈德良张金盈梁红霞唐俊楠王勃李静赵红霞刘闯王佩文靳之崔新月
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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