Eccrine sweat gland cell induction medium and application thereof

A technology for inducing culture medium and sweat gland cells, applied to animal cells, vertebrate cells, artificial cell constructs, etc., can solve the problems of unclear mechanism of action, uncertain sweat gland cells, low efficiency of sweat gland cell isolation and purification, and subculture, etc. To achieve the effect of simple operation steps, easy popularization and simple cultivation equipment

Active Publication Date: 2015-03-25
SUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method also has problems. Due to the low efficiency of sweat gland cell separation, purification and subculture, it is difficult to obtain a large amount of culture supernatant; and the composition of the culture supernatant is not clear, and its specific mechanism of action is also unclear; The sweat gland cells of

Method used

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  • Eccrine sweat gland cell induction medium and application thereof
  • Eccrine sweat gland cell induction medium and application thereof
  • Eccrine sweat gland cell induction medium and application thereof

Examples

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Embodiment 1

[0055] Example 1: Inducing human amniotic fluid stem cells to differentiate into sweat gland-like cells

[0056] Sweat gland cell induction medium, including 450mL DMEM / F12 cell culture medium (Hyclone, SH30023.01B), 500mL keratinocyte medium (KGM2) (Lonza, CC-3107), additionally add 50mL special grade fetal bovine serum, 1mmol / L Triiodothyronine, 0.2 mg / L hydrocortisone, 1% insulin-transferrin-sodium selenite, 1 mmol / L L-glutamine, 0.05 U / L penicillin and 0.05 μg / L streptavidin Add 50 μg / L epidermal growth factor (EGF), 25 μg / L hepatocyte growth factor (HGF) and 10 μg / L bone morphogenetic protein 4 (BMP4).

[0057] Inducing amniotic fluid stem cells to differentiate into sweat gland-like cells, including the following steps:

[0058] (1) Take 5×10 4 Human amniotic fluid stem cells per well are spread in a 6-well plate and cultured using amniotic fluid stem cell medium;

[0059] Amniotic fluid stem cell medium: α-MEM medium supplemented with 15% fetal bovine serum (FBS), 1 ...

Embodiment 2

[0069] Example 2: Inducing Pluripotent Stem Cells to Differentiate into Sweat Gland-like Cells

[0070] Sweat gland cell induction medium, including 450mL DMEM / F12 cell culture medium, 450mL keratinocyte medium (KGM2), additionally added 55mL special grade fetal bovine serum, 1.5mmol / L triiodothyronine, 0.3mg / L hydrocortisone Pine, 2% insulin-transferrin-sodium selenite, 1.5mmol / L L-glutamine, 0.08U / L penicillin and 0.09μg / L streptomycin; then add 55μg / L epidermal growth factor (EGF) , 30 μg / L hepatocyte growth factor (HGF) and 8 μg / L bone morphogenetic protein 4 (BMP4).

[0071] Inducing pluripotent stem cells to differentiate into sweat gland-like cells includes the following steps:

[0072] (1) Take 1×10 5 The pluripotent stem cells per well are spread in a 6-well plate and cultured using a pluripotent stem cell medium;

[0073] Pluripotent stem cell medium: containing 20% ​​KnockOut serum, 1% non-essential amino acids, 0.1mmol / L β-2-mercaptoethanol, 4μg / L FGF-based, 2mm...

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Abstract

The invention discloses an eccrine sweat gland cell induction medium and an application thereof. The eccrine sweat gland cell induction medium comprises a DMEM/F12 cell medium, a keratinocyt medium, fetal calf serum, an epidermal growth factor, triiodothyronine, hydrocortisone, insulin-transferrin-sodium selenite, L-glutamine, penicillin, streptomycin, a hepatocyte growth factor and a bone morphogenetic protein 4. The eccrine sweat gland cell induction medium disclosed by the invention can be used for successfully inducing stem cells to be directionally differentiated to eccrine sweat gland-like cells so as to provide sufficient eccrine sweat gland cells for treating extensive burn patients, so that the living quality of the patients is greatly improved. The medium further provides a theoretical basis for researching differentiated development of the sweat gland, so that the medium has a potential clinical application prospect.

Description

technical field [0001] The invention relates to a sweat gland cell induction medium and its application in inducing stem cells to differentiate into sweat gland-like cells, belonging to the technical field of sweat gland cell culture. Background technique [0002] The annual incidence of burns in my country is about 1.5% to 2%, that is, about 10 million people suffer from different degrees of burns every year, and nearly 10,000 people suffer from severe burns, and about 10% of the patients cannot save their lives due to lack of skin sources. The rescued patients with extensive burns lost their normal skin function for life due to non-functional scar tissue replacing the skin, which seriously affected the quality of life of the patients. The treatment of severe burns on large areas of skin is a medical and social problem to be solved urgently. [0003] The skin is the largest organ in the human body and has functions such as protecting the body, perspiration, and sensing hea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
Inventor 张学光秦明德梁含思李芳孙青
Owner SUZHOU UNIV
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