Straw low temperature degradation acidification microbial agent and preparation method and application thereof

A technology of acidifying bacteria and low temperature, applied in the field of microorganisms, can solve the problems of low efficiency of straw degradation and biogas production, and achieve the effect of strong bacterial viability and improved efficiency

Inactive Publication Date: 2017-01-25
YANBIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a low-temperature degradative acidification bacterial agent for st

Method used

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  • Straw low temperature degradation acidification microbial agent and preparation method and application thereof
  • Straw low temperature degradation acidification microbial agent and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] A low-temperature degrading acidification bacterial agent for straw, including bacterial strains and adsorption carriers, the bacterial strains include: anaerobes, Clostridium cellobiose, Clostridium xylan, and Bacillus cereus;

[0030] Among them, the number of effective viable bacteria of mutual oxygen bacteria such as mutual oxygen monas, cellobiose clostridium, xylan clostridium and bacillus cereus is 10 9 More than one / mL, and the number of colonies of mutual oxygen bacteria: the number of colonies of Clostridium cellobiose: the number of colonies of Clostridium xylan: the number of colonies of Bacillus cereus is 33:33:18:5;

[0031] The adsorption carrier is made of medical stone and straw powder in a mass ratio of 3:7, and the ratio of the total number of effective viable bacteria in the straw low-temperature degradation acidification bacterial agent to the mass of the adsorption carrier is 1×10 6 Each: 1g adsorption carrier.

[0032] The specific implementation...

Embodiment 2

[0041] A low-temperature degrading acidification bacterial agent for straw, including bacterial strains and adsorption carriers, the bacterial strains include: anaerobes, Clostridium cellobiose, Clostridium xylan, and Bacillus cereus;

[0042] Among them, the number of effective viable bacteria of mutual oxygen bacteria such as mutual oxygen monas, cellobiose clostridium, xylan clostridium and bacillus cereus is 10 9More than one / mL, and the number of colonies of mutual oxygen bacteria: the number of colonies of Clostridium cellobiose: the number of colonies of Clostridium xylan: the number of colonies of Bacillus cereus is 35:35:20:10;

[0043] The adsorption carrier is made of medical stone and straw powder in a mass ratio of 3:7, and the ratio of the total number of effective viable bacteria in the straw low-temperature degradation acidification bacterial agent to the mass of the adsorption carrier is 2×10 5 Each: 1g adsorption carrier.

[0044] The specific implementation...

Embodiment 3

[0053] A low-temperature degrading acidification bacterial agent for straw, including bacterial strains and adsorption carriers, the bacterial strains include: anaerobes, Clostridium cellobiose, Clostridium xylan, and Bacillus cereus;

[0054] Among them, the number of effective viable bacteria of mutual oxygen bacteria such as mutual oxygen monas, cellobiose clostridium, xylan clostridium and bacillus cereus is 10 9 More than one / mL, and the number of colonies of mutual oxygen bacteria: the number of colonies of Clostridium cellobiose: the number of colonies of Clostridium xylan: the number of colonies of Bacillus cereus is 36:36:25:15;

[0055] The adsorption carrier is made of medical stone and straw powder in a mass ratio of 3:7, and the ratio of the total number of effective viable bacteria in the straw low-temperature degradation acidification bacterial agent to the mass of the adsorption carrier is 1×10 5 Each: 1g adsorption carrier.

[0056] The specific implementatio...

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Abstract

The invention discloses a straw low temperature degradation acidification microbial agent. The straw low temperature degradation acidification microbial agent contains a bacterial strain and an adsorption carrier, wherein the bacterial strain contains aminobacterium mobile sp, clostridium cellulolyticum, clostridium xylanolyticum and bacillus cereus, wherein the colony count of aminobacterium mobile sp is 33-36, the colony count of clostridium cellulolyticum is 33-36, the colony count of clostridium xylanolyticum is 18-25, and the colony count of bacillus cereus is 5-15; the adsorption carrier is prepared by mixing medical stones and straw powder; and the ratio of the total number of effective live bacteria to the mass of the absorption carrier in the straw low temperature degradation acidification microbial agent is 1*10<5>-1*10<6>: 1g of adsorption carrier. The straw low temperature degradation acidification microbial agent can quickly decompose celluloses and hemicelluloses at a low temperature and degrade the celluloses and the hemicelluloses into acetic acid, thereby increasing biogas production efficiency. In addition, the invention also provides a preparation method and application of the straw low temperature degradation acidification microbial agent.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a low-temperature degrading acidifying bacterial agent for straw, and also relates to a preparation method and an application of the acidifying bacterial agent for low-temperature degradation of straw. Background technique [0002] Biogas, as a renewable energy with negative net emission effect of greenhouse gases, plays an important role in modern energy. The further preparation of biogas from waste through biogas fermentation can not only solve the pollution problem of waste, but also deal with the shortage of fossil energy to a certain extent. Lignocellulosic materials such as straw have become the main raw materials for biogas production. In Europe, there are even energy crops for biogas production. However, the low temperature in the northern cold region is the bottleneck factor of biogas fermentation, which inhibits the application and promotion of bioga...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12P5/02C12R1/145C12R1/085
CPCC12N11/14C12P5/023Y02E50/30
Inventor 赵洪颜王伟东于海茹朴仁哲崔宗均
Owner YANBIAN UNIV
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