The invention relates to a nifuratel-nystatin soft capsule-type suppository and a preparation method thereof. Nifuratel-nystatin sol in the nifuratel-nystatin soft capsule-type suppository is preparedfrom the following components in parts by weight: 40-60 parts of nifuratel, 3-5 parts of nystatin, 2-3 parts of carbomer, 1-2 parts of ethylparaben, 50-70 parts of glycerinum, 70-100 parts of ethyl alcohol, 20-30 parts of pH buffer liquid and 700-800 parts of medical pure water. The preparation method comprises the following steps of 1, raw material preparation; 2, liquid medicine preparation, wherein the nifuratel and the nystatin are mixed evenly in a sterile environment, then through a sterile filtering system, sterile filtering is conducted, and then micro-pore filtering is conducted, sothat a liquid medicine is obtained for later use; 3, gel matrix preparation; 4, nifuratel-nystatin sol preparation; 5, capsule pressing; 6, soft capsule-type suppository preparation. The nifuratel-nystatin soft capsule-type suppository and the preparation method thereof have the advantages that body hormone secretion is not affected, the sterilization effect is good, and the preparation method issimple.