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Colony count card and preparation method thereof

A technology for counting cards and the total number of colonies, which is applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the lack of detection accuracy and color appearance, domestic companies such as Guangzhou Oasis Biochemical Research Center, Beijing Road and Bridge Technology Co., Ltd. and Beijing Liangrun Biotechnology Co., Ltd. and other research and development issues, to achieve the effect of reducing testing costs, convenient access, and less usage

Inactive Publication Date: 2020-09-01
北京华益精点生物技术有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

Domestic companies such as Guangzhou Oasis Biochemical Research Center, Beijing Luqiao Technology Co., Ltd. and Beijing Liangrun Biotechnology Co., Ltd. have developed related disposable detection products, but these products are slightly lacking in detection accuracy and color appearance.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0012] 1. A method for preparing a count card for total number of colonies, comprising the steps of:

[0013] Step 1: Preparation and attachment of the medium on the carrier: Accurately weigh 1.2 g of peptone, 0.2 g of yeast extract, 0.6 g of sodium chloride, 0.9 g of sodium alginate, 7 g of a mixture of potassium dihydrogen phosphate and disodium hydrogen phosphate, 150ul of 1% TTC aqueous solution, add 100ml of pure water, stir to dissolve evenly, spread the evenly dissolved medium on a white synthetic paper of 10cm*10cm, and place it in an environment below 40°C-50°C to dry for use;

[0014] Step 2: Adhesion of the water-absorbing gel on the transparent polyester film: First, apply a layer of self-adhesive on the transparent polyester film with a brush, and then evenly coat the sodium polyacrylate dry powder on the transparent polyester film, and flatten it. You can;

[0015] Step 3: Cut the synthetic paper carrier processed in the first step and the transparent polyester ...

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PUM

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Abstract

The invention discloses a colony count card and a preparation method thereof, and belongs to the technical field of rapid microbial detection. Dry powder of various components of a culture medium is utilized, a certain amount of corresponding indicator is added according to different target microorganisms to prepare a microorganism test piece; a series of tedious preparation work such as traditional culture medium preparation high-pressure steam sterilization and plate pouring is omitted, the manufacturing process is simple, the product is stable and convenient to take and use, professional operators and expensive instruments are not needed, and the working efficiency of microbiological detection personnel can be greatly improved; a proper amount of specific enzyme chromogenic indicator isadopted, so that the chromogenic effect and the result accuracy are ensured; the use amount of the culture medium is small, and the detection cost can be greatly reduced.

Description

technical field [0001] The invention belongs to the technical field of rapid detection of microorganisms, and in particular relates to a counting card for the total number of colonies and a preparation method thereof. Background technique [0002] The total number of bacterial colonies in food directly reflects the content of harmful microorganisms. It is an important task for the health and epidemic prevention department, and it is also one of the important indicators for food manufacturers to conduct product self-inspection, which directly involves food safety issues. The traditional method for determining the microbial contamination of food is the dish pouring method, which relies on the method of target microorganisms in the culture medium to determine whether the food is qualified. A series of operation steps from the molten state to the plate, the operation is cumbersome, time-consuming and labor-intensive; each plate requires at least 15ml of culture medium, which is ...

Claims

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Application Information

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IPC IPC(8): C12Q1/06
CPCC12Q1/06
Inventor 赵广宇张伟陈金辉
Owner 北京华益精点生物技术有限公司
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