Composite bacterial agent for deodorization and preparation method thereof

A technology of composite bacterial agents and seeds, applied in the direction of microorganism-based methods, biochemical equipment and methods, deodorization, etc., can solve the problem of degrading one or more specific pollutants, it is difficult to guarantee the use effect and time, and large-scale Difficulty in use and other problems, to shorten the preparation time of the bacterial agent, improve the deodorization effect and time, and continue to use for a long time

Active Publication Date: 2012-01-18
SHANGHAI MASTECK ENVIRONMENTAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the influence of climate and source, there will be certain differences in the types and quantities of microorganisms in the bark and soil. Although the quality can be controlled by selecting the place of origin, it is still difficult to guarantee the use effect and time of each batch, which has a great impact on industrialization. Scale use brings certain difficulties, so inoculation of deodorizing microorganisms on fillers has become an effective way to improve deodorization effect and service time
Although it is convenient for production and application to inoculate a single strain on the filler, there are many kinds of odorous pollutants that need to be removed. A single strain can only degrade one or several specific pollutants, which cannot meet the actual needs. Compound microbial deodorization The method avoids the above defects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Fermentation of Bacillus subtilis, Thiobacillus intermedia and Bacillus licheniformis:

[0025] (1) Configure the first liquid culture medium, which contains 0.5% glucose, 0.5% beef extract, 0.5% sodium thiosulfate, 0.2% potassium dihydrogen phosphate and magnesium chloride (containing 6 parts by weight) Crystal water) 0.02% aqueous solution, no need to adjust pH.

[0026] (2) Inoculate the three bacilli activated on the agar slant, 20% by weight of Bacillus subtilis, 20% of Thiobacillus intermedia and 60% of Bacillus licheniformis, into the first liquid after high temperature sterilization at 121°C for 20 minutes Cultivate on a shaker in the culture medium, connect 4 bottles in total, 250mL in each bottle. Cultivate at 150r / min, 30°C until the total number of colonies reaches 1×10 8 per ml (calculated by the plate counting method) to prepare the seed culture solution.

[0027] (3) In the fermenter, use the first liquid culture liquid as yeast liquid, prepare 50L,...

Embodiment 2

[0034] 1. Fermentation of Bacillus subtilis, Thiobacillus intermedia and Bacillus licheniformis:

[0035] (1) Configure the first liquid culture medium, which contains 1.0% glucose, 1.0% beef extract, 0.5% sodium thiosulfate, 0.2% potassium dihydrogen phosphate and magnesium chloride (containing 6 parts by weight) Crystal water) 0.02% aqueous solution, no need to adjust pH.

[0036] (2) Inoculate the three bacilli activated on the agar slant into the first liquid after high-temperature sterilization at 121°C for 20 minutes at a ratio of 30% by weight of Bacillus subtilis, 30% of Thiobacillus intermedia and 40% of Bacillus licheniformis Cultivate on a shaking table in the culture medium, connect 3 bottles in total, 250mL in each bottle. Cultivate at 150r / min, 36°C until the total number of colonies reaches 1×10 8 per ml (calculated by the plate counting method) to prepare the seed culture solution.

[0037] (3) In the fermenter, use the first liquid culture liquid as the fer...

Embodiment 3

[0044] 1. Fermentation of Bacillus subtilis, Thiobacillus intermedia and Bacillus licheniformis:

[0045] (1) Configure the first liquid culture medium, which contains 2.0% glucose, 2.0% beef extract, 1.0% sodium thiosulfate, 0.2% potassium dihydrogen phosphate and magnesium chloride (containing 6 parts by weight) Crystal water) 0.02% aqueous solution, no need to adjust pH.

[0046] (2) Inoculate the three bacilli activated on the agar slant at a ratio of 40% by weight of Bacillus subtilis, 30% of Thiobacillus intermedia and 30% of Bacillus licheniformis into the first liquid after high-temperature sterilization at 121°C for 20 minutes Cultivate on a shaking table in the culture medium, connect 3 bottles in total, 250mL in each bottle. Cultivate at 150r / min, 37°C until the total number of colonies reaches 1×10 8 per ml (calculated by the plate counting method) to prepare the seed culture solution.

[0047] (3) In the fermenter, use the first liquid culture liquid as the fer...

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PUM

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Abstract

The invention relates to a composite bacterial agent for deodorization and a preparation method thereof; the composite bacterial agent comprises a bacillus group composed of bacillus subtilis, thiobacillus intermedius, bacillus licheniformis, and a yeast group composed of saccharomyces ellipsoideus and nectaromyces sp. The preparation method comprises the following steps: activating the purchased2 groups of microorganism, preparing a bacterial agent with a total colony count of each group of not less than 1*107/ml by two steps of seed culture and fermenter culture, uniformly distributing the2-group bacterial agents at different areas of fillers in a biological filter tank for deodorization operations, wherein the bacterial agent comprises by weight 30-50% of bacillus groups and 50-70% of yeast groups, and the two groups are not mixed during distribution. The composite bacterial agent for deodorization prepared by the method provided by the invention has the advantages of good deodorization effect and long deodorization period, and the service time is increased by above 30% when compared with that of agents without the addition of the composite bacterial agent.

Description

technical field [0001] The invention relates to a microbial deodorant and a preparation method thereof, in particular to a compound bacterial agent for deodorization and a preparation method thereof. Background technique [0002] All substances that stimulate the smell of olfactory organs are called odor pollutants, among which hydrogen sulfide, ammonia, methane, mercaptans and phenols are more harmful to humans. In addition to causing unpleasant feelings such as psychological disgust, foul-smelling pollutants can also cause symptoms such as nausea, headache, and loss of appetite. Biological filtration method is a relatively new air pollution control method, which uses microorganisms to degrade or transform volatile organic compounds, hydrogen sulfide, and ammonia. It has high treatment efficiency, low infrastructure and operating costs, no need to add chemical reagents, and no secondary pollution. Etc. [0003] In the biological filtration method, the odor is firstly pret...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16A61L9/01B01D53/84B01D53/38C12R1/125C12R1/10C12R1/01C12R1/85A61L101/52
CPCY02A50/20
Inventor 庄田顾宇翔谢坤
Owner SHANGHAI MASTECK ENVIRONMENTAL
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