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Preparation method of fermentation microbial inoculum for reinforcing and promoting organic fertilizer compost

A technology of organic fertilizer and compost, applied in the field of bacterial agent preparation

Inactive Publication Date: 2012-11-28
叶城县金秋实业生物肥有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of bacteria-fungus to strengthen the bacteria agent for composting chicken manure, sludge and garbage and its high-density, high-activity production process have not been reported yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Follow these steps:

[0020] 1) Activate the slant surfaces of the five bacterial strains stored at 4°C, and culture them in test tubes and shake flasks respectively. Bacillus subtilis, Bacillus licheniformis and Rhodopseudomonas palustris were cultured in beef extract peptone medium (recipe: beef extract 5g, peptone 10g, sodium chloride 5g, distilled water 1000mL, pH 7.2-7.4). Saccharomyces cerevisiae and Candida tropicalis were cultured in potato-sucrose medium [Recipe: 200g of peeled and residual potatoes, shredded or sliced, boiled in about 1000mL of tap water at 80-100℃ for 30min, filtered with double gauze, Obtain 1000mL filtrate (insufficient part is supplemented with tap water), add sucrose 20g, natural pH value]. Medium sterilization: 0.1Mpa, 121°C, sterilization for 30min;

[0021] After inoculation, shake at 200r / minz and incubate at 28°C for 24h.

[0022] 2) Secondary liquid expansion culture (30L), expanded culture with cornmeal-soybean meal medium (reci...

Embodiment 2

[0027] Follow these steps:

[0028] 1) Activate the slant planes of the five strains stored at 4°C, and culture them in test tubes and shake flasks respectively. Bacillus subtilis, Bacillus licheniformis and Rhodopseudomonas palustris were cultured in beef extract peptone medium (recipe: beef extract 5g, peptone 10g, sodium chloride 5g, distilled water 1000mL, pH 7.2-7.4). Saccharomyces cerevisiae and Candida tropicalis were cultured in potato-sucrose medium [Recipe: 200g of peeled and residual potatoes, shredded or sliced, boiled in about 1000mL of tap water at 80-100℃ for 30min, filtered with double gauze, Obtain 1000mL filtrate (insufficient part is supplemented with tap water), add sucrose 20g, natural pH value]. Medium sterilization: 0.1Mpa, 121°C, sterilization for 30min;

[0029] After inoculation, shake at 200r / minz and incubate at 28°C for 24h.

[0030] 2) Secondary liquid expansion culture (50L fermenter), expanded culture with cornmeal-soybean meal medium (recipe...

Embodiment 3

[0035] Follow these steps:

[0036] 1) Activate the slant surfaces of the five bacterial strains stored at 4°C, and culture them in test tubes and shake flasks respectively. Bacillus subtilis, Bacillus licheniformis and Rhodopseudomonas palustris were cultured in beef extract peptone medium (recipe: beef extract 5g, peptone 10g, sodium chloride 5g, distilled water 1000mL, pH 7.2-7.4). Saccharomyces cerevisiae and Candida tropicalis were cultured in potato-sucrose medium [Recipe: 200g of peeled and residual potatoes, shredded or sliced, boiled in about 1000mL of tap water at 80-100℃ for 30min, filtered with double gauze, Obtain 1000mL filtrate (insufficient part is supplemented with tap water), add sucrose 20g, natural pH value]. Medium sterilization: 0.1Mpa, 121°C, sterilization for 30min;

[0037] After inoculation, shake at 200r / minz and incubate at 28°C for 24h.

[0038] 2) Secondary liquid expansion culture (20L), expanded culture with cornmeal-soybean meal medium (reci...

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PUM

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Abstract

The invention relates to a preparation method of a fermentation microbial inoculum for reinforcing and promoting organic fertilizer compost. The method provided by the invention is characterized in that the microbial inoculum of the invention has compost promotion effects on chicken manure, sludge and garbage, and is complex microorganisms composed of bacillus subtilis, Bacillus licheniformis, Rhodopseudomonas palustris, saccharomyces cerevisiae and candida tropicalis with a ratio of 1:1:1:1:1 basically. The method has the active effects that 0.5-1% of microbial inoculum is added in a compost material (chicken manure, sludge or house refuse), and a micro-ecological dominant microflora can be formed rapidly. According to the invention, the odor of a compost material with water content of 50%-60% can be eliminated on the second day (determination by character description) under normal temperature, and the compost process can be completed in 5-7 days. The fermentation microbial inoculum has a high effective colony count and strong adaptability, and has obvious effects for eliminating the odor and promoting the composting process.

Description

technical field [0001] In the field of biological technology, the present invention relates to a preparation method of a bacterial agent that strengthens and promotes the composting fermentation process of organic fertilizer. Background technique [0002] Today, when there is a large demand for organic fertilizers, it is a development trend to compost chicken manure, sludge and domestic waste into decomposed organic fertilizers. However, in the process of natural composting, it often takes months or even years, not only cannot be fully decomposed, but also emits stench continuously, pollutes the surrounding environment, and leaves harmful bacteria and insect eggs. Direct application will cause soil-borne diseases and insect pests. In order to change this situation, bacteria that can degrade ammonia (amine) are used to degrade and remove odorous substances. At the same time, bacteria and fungi cooperate to promote aerobic fermentation at various stages of the composting proce...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/16C12N1/18C12N1/20C05F17/00C05F9/04C12R1/10C12R1/125C12R1/01C12R1/865C12R1/74
CPCY02A40/20Y02W30/40
Inventor 张清敏刘志军李明张彦峰
Owner 叶城县金秋实业生物肥有限公司
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