Method for measuring bacterial colonies of bacillus thuringiensis in refuse compost

Abstract

The invention discloses a method for measuring bacterial colonies of bacillus thuringiensis in refuse compost. According to the method, the average number of bacterial colonies in the same dilution is calculated firstly, if the size of sheet-shaped lawns is less than half that of a panel and the distribution of the other half of bacterial colonies is even, then the number of the bacterial colonies of the whole panel can be expressed by multiplying half the number of the bacterial colonies by 2, then the average number of the bacterial in the dilution is calculated, the bacterial colonies with the average number of the bacterial colonies between 30 and 300 are selected, and when the average number of the bacterial colonies in only one dilution accords with the range, the total number of the microorganism bacterial colonies of a sample is expressed by multiplying the average number of the bacterial colonies by the dilution factor. Experimental results show that the bacterial colonies formed on a culture medium are round, white and non-transparent, are smooth in edges, and are positive by gram stain; observed under a microscope, the bacteria are in oval rhabditiform and bacterial spores are oval; and bromophenol blue and sarranine dye liquor are used for conducting parasporal crystal dye, red bacteria, colourless bacteria spores and blue parasporal crystals are observed under the microscope, therefore, the bacteria are preliminarily evaluated as bacillus thuringiensis.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and relates to a method for determining Bacillus thuringiensis colony in garbage compost. Background technique [0002] Composting refers to the process of degrading organic waste under the action of microorganisms (mainly bacteria) and transforming organic matter into stable humus under controlled conditions. The product after composting is called compost. The composting process can effectively reduce the volume of the mixture by 40%-50%, and can rely on the heat generated by metabolism in the high temperature stage to kill the pathogenic substances in the garbage. Composting is not a new technology, but it is in the interest of the environment to treat MSW in this way, because the composting process removes or reduces the toxicity of MSW and allows the final product to be reused . The composting procedure includes raw material pretreatment, raw material fermentation and post...

AI Technical Summary

Problems solved by technology

However, there are very complex microbial systems in composting, and the changes and succession of microbial structures in the entire composting process are also very large. And the innovation of research methods, the application of new technologies such as molecular biology methods, hybridization technology, PCR and DNA fingerprinting provide a new way for the research of compost microbiology

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