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Quick and simple leafhopper digestive organ anatomy method

A simple technique for digestive organs, applied in the field of insect anatomy, can solve the problems of wasting experimental materials, large damage to the digestive tract, affecting experimental results and quality, and achieve the effect of preserving integrity

Inactive Publication Date: 2014-05-21
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method not only causes great damage to the digestive tract, which affects the experimental results and quality, but also is very difficult, because the digestive tract is often rolled up in the dermis when peeled from the outside to the inside, and it is not easy to detect. Sometimes the whole leafhopper is peeled off. The digestive tract cannot be found, wasting experimental materials

Method used

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  • Quick and simple leafhopper digestive organ anatomy method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Place the collected leafhopper nymphs in glass test tubes and paralyze them on ice.

[0021] 2. Put 1 drop of PBS on the slide and place it under a stereo microscope.

[0022] 3. Transfer the paralyzed leafhoppers to a glass slide in PBS, belly side up.

[0023] 4. Use the tweezers with your left hand to lightly clamp the worm body for fixation, and use the tweezers with your right hand to clamp the head and move it slightly to the right so that the head is separated from the body, but the esophagus is still connected to the head and the chest and abdomen. Continue to tighten the tweezers with the right hand, move carefully to the right, and gradually pull out most of the digestive tract (midgut). With the continuous movement of the right hand, the end of the digestive tract originally connected to the tail will separate from the tail, and finally the complete digestive tract organs (midgut and hindgut) will be obtained without extensive damage to the body of the le...

Embodiment 2

[0025] 1. Place the collected adult female leafhoppers in glass test tubes and paralyze them on ice.

[0026] 2. Put 1 drop of PBS on the slide and place it under a stereo microscope.

[0027] 3. Move the paralyzed leafhoppers into PBS on a glass slide with the abdomen facing up.

[0028] 4. Use tweezers with your left hand to lightly pinch the worm body for fixation, and use tweezers with your right hand to remove the head. Then, use the right hand to clamp the side of the penultimate segment of the abdominal segment, and the left hand to clamp the ovipositor at the tail, and move slowly to the left carefully, gradually tearing the intersegmental membrane of this abdominal segment, exposing the digestive tract organ (midgut). Continue to move the left hand to the left, and gradually pull out the complete digestive tract located in the abdomen. At this time, the end of the intestine is still connected to the tail, so use tweezers to slightly break the end of the intestine c...

Embodiment 3

[0030] 1. Place the collected male adult leafhoppers in glass test tubes and paralyze them on ice.

[0031] 2. Put 1 drop of PBS on the slide and place it under a stereo microscope.

[0032] 3. Move the paralyzed leafhoppers into PBS on a glass slide with the abdomen facing up.

[0033] 4. Use tweezers with your left hand to lightly pinch the worm body for fixation, and use tweezers with your right hand to remove the head. Then, use tweezers with your right hand to hold the side of the penultimate segment of the abdominal segment, and use tweezers with your left hand to hold the genitalia at the tail, and move slowly to the left carefully, gradually tearing the intersegmental membrane of this abdominal segment, exposing the organs of the digestive tract (midgut). Continue to move the left hand to the left, and gradually pull out the complete digestive tract located in the abdomen. At this time, the end of the intestine is still connected to the tail, so use tweezers to slig...

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Abstract

The invention provides a quick and simple leafhopper digestive organ anatomy method and belongs to the field of entomotomy. According to the method, a complete leafhopper digestive tract can be obtained in a short time, losses of components of cells can be reduced to the greatest degree, it can be guaranteed that initial conditions of ultrastructures in the cells can be kept complete, and good results in the next step of experiment can be obtained conveniently. According to the method, the digestive tract is connected with salivary glands of the head and the rectum of the tail, the digestive tract can be dissected out carefully through a drawing and pulling method, digestive organs are not damaged, leafhopper shells are not destroyed, and the completeness of insects are reserved to the greatest degree.

Description

technical field [0001] The invention relates to a quick and convenient method for dissecting the digestive organs of leafhoppers, belonging to the field of insect anatomy. Background technique [0002] Anatomy is the branch of biology concerned with the structure and organization of living organisms; it can be divided into animal anatomy and plant anatomy. The main branches of anatomy are comparative anatomy, histology, and human anatomy. In anatomical research, dissection is often used to study gross organs; microscopes are used to observe tissues, cells, and organelles. [0003] Insect anatomy is an important technique to study the interaction mechanism between mediators and pathogens, and it is the premise of electron microscopy and immunofluorescence observation of pathogens invading the digestive tract experiments. Leafhoppers are relatively common vector insects in this research. This kind of insect is small in size, the body length of the 3rd instar nymph is about ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D1/00
Inventor 陈红燕魏太云陈倩毛倩卓贾东升吴维
Owner FUJIAN AGRI & FORESTRY UNIV
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