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Transmission electron microscope sample preparation method of microminiature insect compound eyes

A transmission electron microscope sample, micro-sized technology, applied in the direction of material analysis, measuring devices, instruments, etc., using wave/particle radiation, can solve the problems that cannot be applied to micro-sized insects, etc., and achieve clear and complete microscopic ultrastructure of compound eye slices. Effect of reducing usage and reducing damage

Inactive Publication Date: 2018-08-07
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the defects and insufficiencies that the existing insect compound eye transmission electron microscope sample preparation method cannot be applied to micro-sized insects, and propose a transmission electron microscope sample preparation method for micro-miniature insect compound eyes, especially a thistle Preparation method of TEM sample of equine compound eye

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  • Transmission electron microscope sample preparation method of microminiature insect compound eyes
  • Transmission electron microscope sample preparation method of microminiature insect compound eyes
  • Transmission electron microscope sample preparation method of microminiature insect compound eyes

Examples

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Embodiment 1

[0043] Example 1 Preparation of the compound eye transmission electron microscope sample of common large thrips

[0044] 1. Method

[0045] 1. Reagent preparation:

[0046] A. Preparation of 500mL 0.1M PBS buffer solution: first prepare solutions a and b: solution a (0.2M disodium hydrogen phosphate solution): weigh Na 2 HPO 4 12H 2 O 35.28 grams, add double distilled water to 500mL to complete the preparation; solution b (0.2M sodium dihydrogen phosphate solution): weigh NaH 2 PO 4 2H 2 O 15.605 grams, add double distilled water to 500mL to complete the preparation. Then take 72mL of solution a and 28mL of solution b and mix to make 0.2M PBS stock solution, add 1 times of double distilled water to get 0.1M PBS buffer solution (pH 7.2).

[0047] B. Preparation of 100mL 2.5% glutaraldehyde fixative: For each 100mL fixative, mix 10mL of 25% glutaraldehyde aqueous solution with 50mL of PBS buffer, add double distilled water to make up to 100mL.

[0048] C. Osmic acid prep...

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Abstract

The invention discloses a transmission electron microscope sample preparation method of microminiature insect compound eyes. The method includes the steps of S1, cleaning an insect body; S2, sampling;S3, fixing and rinsing; S4, fixing and rinsing again; S5, dewatering and replacing; S6, performing permeation sample soaking; S7, performing polymerization drying; S8, positioning, slicing, and drying slices; S9, dyeing and observing. The transmission electron microscope sample preparation method has the advantages that the method is based on an existing sample preparation method, and operation and treatment manners are improved according to microminiature insect tissue features; transmission electron microscope observation shows that a sample prepared by the method is clear and complete in ommatidium ultrastructure and has no evident defects, content loss, deformation and the like, so that the method is applicable to the transmission electron microscope sample preparation of the microminiature insect compound eyes and especially applicable to the transmission electron microscope sample preparation of thrips compound eyes.

Description

technical field [0001] The invention relates to the technical field of biological sample preparation, more specifically, to a transmission electron microscope sample preparation method of tiny insect compound eyes. Background technique [0002] Thrips are Thysanoptera ( Thysanotpera ) is a general term for insects, with a body length of about 0.5-5 mm, belonging to microscopic insects, which can be divided into three categories: fungivorous, predatory, and herbivorous according to their feeding habits. Many herbivorous thrips are important agricultural and forestry pests, such as western flower thrips, common large thrips, flower thrips, rice thrips, etc. Thrips pests are rampant. Due to their small size, strong concealment, and strong fecundity, the conventional chemical control methods of spraying insecticides cannot achieve good control effects. Instead, the thrips populations in many areas have produced varying degrees. resistance to antibiotics and cause serious conta...

Claims

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Application Information

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IPC IPC(8): G01N23/2202
CPCG01N23/2202
Inventor 罗雪君闫凯莉唐良德吴建辉
Owner SOUTH CHINA AGRI UNIV
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