The invention belongs to the technical field of
cell culture and particularly discloses a medical automatic
cell production device and a production method thereof. The medical automatic
cell production device comprises a laminar-flow sterile sealed chamber, in which a sample
bottle (such as a blood
bottle) is arranged; and a
cell separation device for separating samples, a cell rinsing device, a cell induction culture device, a cell proliferation culture device and cell filtering, rinsing and recycling devices for filtering, rinsing and recycling cultured cells are connected through connecting pipes in sequence behind the sample
bottle in the sealed chamber. By the invention, the
cell separation, the induction culture, the large-scale proliferation culture, cell filtering, rinsing and recycling processes and the like of each patient can be completed in an independent
system which is completely sealed and sterile, and therefore the constant conditions of a
cell culture environment are ensured, the
effector cell proportion is greatly improved, a GMP standard is reached and treatment requirement of patients can be satisfied. In addition, the medical cell production method has high
automation degree, simple and convenient operation process, small operation error and high
repeatability and can be widely applied to different working environments and occasions, such as
medical treatment, biopharming and the like.