Medical automatic cell production device and production method thereof

Abstract

The invention belongs to the technical field of cell culture and particularly discloses a medical automatic cell production device and a production method thereof. The medical automatic cell production device comprises a laminar-flow sterile sealed chamber, in which a sample bottle (such as a blood bottle) is arranged; and a cell separation device for separating samples, a cell rinsing device, a cell induction culture device, a cell proliferation culture device and cell filtering, rinsing and recycling devices for filtering, rinsing and recycling cultured cells are connected through connecting pipes in sequence behind the sample bottle in the sealed chamber. By the invention, the cell separation, the induction culture, the large-scale proliferation culture, cell filtering, rinsing and recycling processes and the like of each patient can be completed in an independent system which is completely sealed and sterile, and therefore the constant conditions of a cell culture environment are ensured, the effector cell proportion is greatly improved, a GMP standard is reached and treatment requirement of patients can be satisfied. In addition, the medical cell production method has high automation degree, simple and convenient operation process, small operation error and high repeatability and can be widely applied to different working environments and occasions, such as medical treatment, biopharming and the like.

Description

technical field [0001] The invention belongs to the technical field of cell culture. Specifically disclosed are a medical cell production device and a production method thereof. Background technique [0002] In the field of medical technology, immune cell therapy is to cultivate the patient's own lymphocytes with cancer-killing effect in vitro, induce and expand them by thousands of times, and then reinfuse them into the patient's body to enhance the body's own internal immune anti-cancer ability, so as to achieve The purpose of treating and preventing tumors, it can improve cancer treatment to a high level of cellular immunotherapy, and has a good effect on treating tumors. [0003] In the prior art, the production process of some medical cells used for immune cell therapy is generally completed manually, the operation process is cumbersome, and microbial contamination and cross-contamination are prone to occur during the operation process, which cannot ensure the aseptic ...

AI Technical Summary

Problems solved by technology

[0003] In the prior art, the production process of some medical cells used for immune cell therapy is generally completed manually, the operation process is cumbersome, and microbial contamination and cross-contamination are prone to occur during the operation process, which cannot ensure the aseptic production of the entire process; artificial cultivation methods , high requirements for operators, low repeatability, and unstable quality; at the same time, the existing medical cell culture methods have a small number of cell induction and expansion, a low proportion of effector cells, and poor cell immunotherapy effects, so it is difficult to be widely used.

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