BRAF gene mutation detection system and kit thereof

A detection kit and system technology, applied in the biological field, can solve the problems of affecting the accuracy of detection results, difficulty in large-scale promotion and application, expensive equipment, etc., to meet the clinical screening of large sample size, low price, and reduce detection costs Effect

Pending Publication Date: 2018-03-06
上海赛安生物医药科技股份有限公司
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Problems solved by technology

[0004] At present, technologies such as next-generation sequencing, FLP, allele-specific oligonucleotide hybridization, allele-specific PCR, and gene chips are mainly used to detect human BRAF gene mutations. , oft

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  • BRAF gene mutation detection system and kit thereof
  • BRAF gene mutation detection system and kit thereof
  • BRAF gene mutation detection system and kit thereof

Examples

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Embodiment Construction

[0031] The present invention is specifically described below through the examples, it is necessary to point out that the following examples are only used to further illustrate the present invention, and can not be interpreted as limiting the protection scope of the present invention, those skilled in the art can according to the above-mentioned present invention Contents Some non-essential improvements and adjustments are made to the present invention. In the following examples, unless otherwise specified, all reagents used are of analytical grade, and all reagents used can be obtained from commercial sources. For the experimental methods that do not indicate the specific conditions in the text, usually follow the conditions described in the book "Molecular Cloning Experiment Guide" published by Science Press, edited by J. Sambrook et al. in 2002, or according to the manufacturer's suggestion conditions of. Unless otherwise defined, all professional and scientific terms used ...

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Abstract

The invention relates to a BRAF gene mutation detection system and a kit thereof. The kit comprises a PCR detection liquid and SYBR GreenI mixed liquor, wherein the PCR detection liquid comprises forward and reverse primers and peptide nucleic acid which are used for detecting a site V60OE of a 600th codon of a BRAF gene; and a nucleotide sequence of the peptide nucleic acid is completely complementary with the site V60OE of the 600th codon of a BRAF wild-type gene. The BRAF gene mutation detection system and the kit thereof have high sensitivity and specificity, demanded sample quantity is low, and BRAF gene mutation detection can be quickly, conveniently and accurately carried out.

Description

technical field [0001] The invention relates to a method for detecting BRAF gene mutation and detection products, belonging to the field of biotechnology. Background technique [0002] The BRAF gene is located on chromosome 7q34 and is one of the most important proto-oncogenes in humans. It encodes a serine / threonine kinase and is an important transduction factor in the EGFR pathway RAS / RAF / MEK / MRK / MAPK and participates in the regulation of cell growth , differentiation and apoptosis and other physiological processes. Studies have shown that there are different rates of BRAF gene mutations in various malignant tumors such as malignant melanoma, lung cancer, and colorectal cancer, among which about 82% of the mutations are located at the 1799th nucleotide of exon 15, and the T mutation is A (T1799A ), causing the glutamic acid encoded by it to be replaced by valine (V600E). This mutation activates the BRAF protein and leads to the continuous activation of the downstream MEK-...

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Application Information

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IPC IPC(8): C12Q1/6858
CPCC12Q1/6858C12Q2563/107C12Q2531/113C12Q2525/203C12Q2525/107
Inventor 王明赵国玲
Owner 上海赛安生物医药科技股份有限公司
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