37 results about "BRAF Protein" patented technology

The present invention relates to probiotic microorganisms producing chimeric human growth hormone for oral use and methods for preparing them.

The invention provides probiotic Lactobacillus or yeast transformant expressing chimeric protein which is human growth hormone fused with Fc fragment of human IgG, in which the transformants are safely delivered into intestine though oral route. Also, the invention provides a chimeric protein-expressing vector which can induce transcytosis in intestine epithelial cells.

Accordingly, the invention demonstrates that the chimeric protein for oral delivery system can be absorbed in intestine, and delivery of the chimeric protein by oral route using Lactobacillus has very excellent efficiency in vivo test in rats. Accordingly, the Lactobacillus of the present invention is an excellent deliverer of protein drugs.

A method of combining ES (ElectroSpray Ionization) and MALDI (Matrix Assisted Laser Desorption Ionization) to create enhanced MALDI mass spectrometry to be referred to as ESMALDI (ElectroSpray Matrix Assisted Laser Desorption Ionization). ESMALDI technology offers substantial advantages over conventional technology. essentially by combining the high ionization efficiency and multiple charging of ions in ESI with the smaller sample requirements and operational simplicity of MALDI. Biologists should thus be able to achieve higher sensitivity, better protein identification, enhanced quantification potential and enhanced structural information. An additional degree of freedom for ESMALDI MS is flexibility in the choice of solvents that can be used in the ESI component of the process. Substantial differences in ESI behavior can occur with the same analyte or mixture of analytes in different solvents. Such differences often provide clues to the properties of the analyte and its ions which cannot be readily obtained from conventional MALDI experiments.

The present invention provides electrophoresis gels and buffers for protein or nucleic acid electrophoresis comprising the compound of Formula I:

wherein R′ is a C₁-C₆ alkyl substituted with SO₃H and optionally substituted with OH; if X=0, R is a pair of electrons; and if X=N, R is R′ and salts and solvates thereof.. The present invention also provides pre-cast gels and pre-mixed gel solutions comprising the compound of formula I. The present invention further provides methods of performing electrophoresis using the gels and buffers comprising a compound of formula I. The gels and buffers comprising a compound of Formula I offer extended shelf life and good protein and nucleic acid resolution.

A method for producing a shelf-stable protein-based pellet that is capable of expansion into a light, crispy snack cake, while providing a good source of protein and calcium. The method, in preferred embodiments, involves making dough from tapioca and potato starches and a milk protein derivative consisting of whey protein isolate, milk protein isolate or calcium caseinate. The mixture is extruded, sliced and dried in a series of dyers. The method produces a shelf-stable pellet having a moisture level of approximately 9-13% by weight which is further processed to produce a puffed dairy protein snack product, having a moisture level typically less than 2%.

The invention discloses a method which adopts tannin to process silkworm cocoon processing wastewater and recycle sericin protein. The method is characterized in that: firstly, tannin solution is prepared by a conventional method; secondly, under the condition of stirring, sulphuric acid or hydrochloric acid is added to wastewater containing sericin; thirdly, under the condition of complete stirring, the tannin solution is added until the sericin protein is completely precipitated, and the turbid wastewater can be clarified; fourthly, standing is carried out for solid-liquid separation; fifthly, after sand in water is filtered, the wastewater reaches the grade 2 discharge standard and then is discharged or recycled; and sixthly, solid substances are cleaned and treated by alcohol to causethe solid-liquid separation, and after the solid is dried the sericin protein can be obtained. The method has the advantages that the tannin is the pure natural and environment-friendly protein flocculant and is available; tannin can fast and efficiently act with the sericin protein, the sericin protein can be separated out more completely than other methods, the formed floccule is easy to precipitate, the separation technology is simple, and the COD removal rate is high.

The invention discloses a method, system, and device for calculating binding free energy of proteins and drugs, and a medium. The method includes the steps: constructing a function of the binding freeenergy of proteins and drugs, wherein the function of the binding free energy of the proteins and the drugs is a relationship between the binding free energy of the proteins and the drugs and an electrostatic item, a polar solvation energy item, a van der Waals item, a nonpolar solvation energy item and interaction entropy; collecting each energy item of each protein and each drug complex in a training set, and performing multivariate linear fitting on the function of the binding free energy of proteins and drugs by using each energy item of each protein and each drug complex in the trainingset and the experimental value of each protein and each drug to obtain a function of the binding free energy of the proteins and the drugs; and inputting each energy item of the proteins to be testedand the drug complex into the trained function of the binding free energy of proteins and drugs, and outputting the binding free energy of the proteins and the drugs.

A process for enzymolyzing the protein to obtain the high-activity enzymolyzed liquid containing amino acids, micro-molecular peptide, nucleotide, glycerine and fatty acids includes washing earthworm or gains for removing impurities, proportionally mixing it with neutral proteinase and/or alkaline proteinase, adding buffer liquid to regulate pH value, and reacting at 20-75 deg.C for at least 2 hrs.

The invention relates to a method for preparing layer-by-layer self-assembled protein-imprinted polymer of chitosan. A cross-linked chitosan spherical particle substrate is first prepared, electronegative template protein BSA and electropositive chitosan are alternately adsorbed by the method of layer-by-layer self-assembly, that is, the template protein and the chitosan are alternately electrostatically self-assembled on the surface of the cross-linked chitosan spherical particle substrate, the imprinting of template protein molecules is carried out, eluent is then adopted to remove the template protein molecules, so that a site and hole structure which can identify the template protein and vice versa is left on the surface of the substrate, consequently, the substrate has the selective adsorption and separation functions of the protein, and the layer-by-layer self-assembled protein-imprinted polymer of chitosan is obtained by the method. The polymer prepared by the method has good capability to selectively adsorb the template protein, and has a good selective protein separation function, meanwhile, the preparation method is simple, preparation conditions are mild, and the method has good recyclability.

A calibration aid may be assembled as follows. First, the standard reference substance is prepared by dissolving ICG dye and albumin protein in water. Then, the carrier sheet of fleece material is soaked therein and dried. After drying the carrier sheet, a thin well defined layer of protein bound dye is present at the surface of the fleece material. The carrier sheet and the backing sheet are laminated into a plastic card. For this, the plastic layers may be laminated tightly together in the framing region for example by welding or by use of adhesive. The plastic card is then sterilized and packed into a sealed package.

A chitosan and metal copper ion complex protein imprinting polymer, using the complex formed by natural polymer chitosan and metal copper ion as a functional monomer, imprinting template protein molecules through molecular imprinting technology, and preparing protein selective Western blotting polymers with strong adsorption and separation functions. The polymer also utilizes the interaction between chitosan molecules and template protein molecules, as well as metal copper ions and template protein molecules, effectively increasing the recognition sites for the combination of imprinted polymers and template protein molecules, thereby improving the imprinted polymers. The ability to recognize template protein molecules and the ability to selectively adsorb and separate template protein molecules. The imprinted polymer has good protein selective adsorption and separation effects, so it can be used as a protein-specific selective adsorption and separation material. At the same time, the preparation method of the material is simple and convenient, easy to control, good reusable effect, and low cost.

The invention provides a graphene modified sodium alginate-polyacrylonitrile heterogeneous enhanced type hollow fiber membrane, particularly relates to a graphene modified sodium alginate-polyacrylonitrile hollow fiber membrane. The membrane is heterogeneously enhanced, the fiber breaking probability is reduced, the membrane can be used for membrane distillation with water and protein media and has higher vapor permeability, excellent separation property, better protein filtration flux and water impermeability under high pressure, and the mechanical property of the membrane is improved greatly. The tensile breaking strength and the selectivity of the membrane are improved greatly by modifying a sodium alginate layer with graphene and heterogeneously enhancing the membrane.

The invention provides a soluble amidated soybean polysaccharide and a preparation method thereof, wherein the soluble amidated soybean polysaccharide has a soluble soybean polysaccharide main chain structure, and the methoxy group in the main chain structure is partially substituted with the amide group. With the technical scheme, the methoxy group in the main chain structure is partially substituted with the amide group to make the main chain structure have the amide group, the amide group introduced through the amidation can improve the stability, and mainly the amide group can help the formation of the hydrogen bond, such that the combination of soybean polysaccharide and casein is stable so as to improve the suspension and the stability. According to the present invention, the methoxygroup in the soybean polysaccharide main chain is substituted with the amide group while the beta-elimination reaction possibly occurring is effectively suppressed so as to control the beta-elimination reaction affecting the quality and the performance of the soybean polysaccharide at a small degree; and the soluble amidated soybean polysaccharide can exhibit good protein stability performance even in the case of the low addition amount.

The invention discloses a Bacillus thuringiensis DavIV and application thereof. The strain is deposited in China Center for Type Culture Collection on September 30, 2018, and the preservation number is CCTCC NO:M 2018626. The strain has nicked edges, a round shape and a relatively moist surface, and is difficult to pick. Tests prove that the Bacillus thuringiensis DavIV has good protein decomposition ability.

The invention provides a biological marker for brain damage induced by microwave radiation. The inventor finds that the expression level of bandicoot hippocampal tissue Braf protein after microwave radiation is significantly increased compared with that before microwave radiation, so that the Braf protein can be used as the biological marker for brain damage induced by microwave radiation.

The invention discloses a method for improving the solubility of zein through subcritical water modification, and belongs to the technical field of food/agricultural products and by-products thereof. The method comprises the following specific steps of subcritical water modification, filtration, vacuum concentration and freeze drying. The dissolution characteristic of the obtained zein is superior to that of untreated zein. Treatment of a water-insoluble protein is carried out by adopting the green, safe and environment-friendly technical means of subcritical water modification, so that the water-insoluble protein is transformed into a soluble protein, and the method is an environment-friendly protein-modifying technique. The method is suitable for research on improvement of the solubility of the protein in all raw materials.