37 results about "BRAF Protein" patented technology

The invention relates to a method for producing a protein-containing food ingredient, according to which flax coarse meal having a low residual oil content is produced or prepared, parts of native flax protein and soluble roughage from said flax coarse meal being dissolved or at least dispersed by aqueous extraction in an aqueous phase. Unsoluble fractions of flax coarse meal are removed from the total aqueous extract produced. A flax-protein-roughage mixture is then separated from the aqueous phase, in order to obtain the food ingredient therefrom. The food ingredient thereby obtained has a neutral taste, is technologically functional and stable in storage, and is characterised by good protein solubility and excellent boundary surface properties.

An antibacterial experiment method capable of determining the molecular weight of antibacterial protein fragments, which relates to the field of microbiological technology; after preparing reagents, separating gels and stacking gels, protein electrophoresis is performed on the proteins subjected to antibacterial experiments, and the obtained protein electrophoresis films are decolorized and stained. After the molecular weight of the protein was observed by imaging, it was used for antibacterial experiments; the protein electrophoresis film was placed in PBS buffer for 10 minutes, and then placed in an agar dish; the bacterial solution in the logarithmic growth phase was added to a 50°C In the nutrient agar medium, obtain a bacterial solution with a volume concentration of 1%; take the obtained bacterial solution and pour it into a petri dish with decolorized protein electrophoresis film, observe the growth of the bacterial cells in the medium, and confirm that the antibacterial effect is good The molecular weight of protein fragments. The invention adopts a simple, accurate, fast and cheap method to determine the molecular weight of protein fragments with antibacterial effect, and has broad application prospects.

The invention discloses the application of a compound in the preparation of antitumor drugs. The inventor's research found that ZINC65387069 has strong kinase inhibitory activity, relatively high water solubility, low polar surface area, and small molecular weight and molecular volume. The phosphorylation level of BRAF protein in colorectal cancer cells treated with ZINC65387069 was significantly inhibited. By detecting the effect of ZINC65387069 on colorectal cancer cell migration, apoptosis and cytoskeleton, ZINC65387069 can inhibit cell migration, destroy cytoskeleton and promote cell apoptosis. ZINC65387069 has the characteristics of simple structure and small molecular weight, which is expected to be used as a lead compound for further structural optimization, and then to develop more effective anti-tumor drugs.