Braf protein of biological marker for detecting brain damage induced by microwave radiation

A biomarker and microwave radiation technology, which is applied in the field of Braf protein, a biomarker for detecting brain damage caused by microwave radiation, can solve problems such as deficiency, and achieve better and more significant effects in treatment or prevention

Pending Publication Date: 2020-02-07
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, with the development of quantitative proteomics technology, accurate quantification of proteins in rat brain tissue can be achieved, but there is a lack of sensitive biomarkers for microwave radiation-induced brain damage

Method used

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  • Braf protein of biological marker for detecting brain damage induced by microwave radiation
  • Braf protein of biological marker for detecting brain damage induced by microwave radiation
  • Braf protein of biological marker for detecting brain damage induced by microwave radiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1 carries out microwave radiation to rat

[0051] 80 secondary male Wistar rats (Academy of Military Medical Research) with a body weight of 220 ± 20 g were randomly divided into 4 groups (C, S, L and SL), and Group C was placed in a radiation box without radiation, and Group S , L and SL groups were exposed to 10mW / cm 2 The S-band, L-band and S and L-band microwave composite radiation, the radiation time is 6 minutes.

Embodiment 2

[0052] Example 2 rat learning and memory function detection

[0053] The SLY-WMS Morris water maze test system (purchased from Beijing Shuolinyuan Technology Co., Ltd., mainly consists of a stainless steel circular pool, a camera, and software is used. The inner wall of the stainless steel circular pool is dark, with a diameter of 120 cm, a height of 50 cm, a water depth of 20 cm, and a water temperature of 20 cm. Controlled at 23±2 degrees Celsius), divide the pool into 4 quadrants, which are designated as 1, 2, 3, and 4 quadrants, and mark 4 water entry points in the middle of the wall of each quadrant. Place a dark platform with a diameter of 12cm and a height of 19cm. A camera is installed above the maze, and by connecting with a computer, the swimming track of the rats is automatically recorded for analysis.

[0054] Rats in each group were trained for 2 days before microwave irradiation, and the rats were put into the water from 4 water entry points facing the pool wall...

Embodiment 3

[0060] Embodiment 3 Detection of EEG Activity in Rats

[0061] Select 5 rats in each group, inject 0.5mL / 100g of 1% pentobarbital sodium intraperitoneally, and the rats are in a state of mild anesthesia (with pain reflex) after 5 minutes, and use the MP-150 multiconductor physiological record produced by BIOPAC company And the analysis system, the electroencephalogram (EEG) of the rats was detected at the 6th hour after the routine feeding was resumed. The specific operation process is as follows: use a hair clipper to remove the hair on the top of the head, disinfect and remove fat, place one electrode on the left and right sides of the center of the top of the head, fix it on the scalp with a needle electrode, insert the reference electrode at the edge of the earlobe on the same side, and connect the electrodes EEG amplifier, and divide the EEG graph into Alpha(α), Beta(β), Theta(θ), delta(δ), with a sensitivity of 2000Hz, continuously record the changes of EEG, and finally ...

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PUM

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Abstract

The invention provides a biological marker for brain damage induced by microwave radiation. The inventor finds that the expression level of bandicoot hippocampal tissue Braf protein after microwave radiation is significantly increased compared with that before microwave radiation, so that the Braf protein can be used as the biological marker for brain damage induced by microwave radiation.

Description

technical field [0001] The present invention relates to the field of biomedicine, specifically, the present invention relates to a biomarker Braf protein for detecting microwave radiation-induced brain damage and its application, more specifically, the present invention relates to a biomarker for detecting microwave radiation-induced brain damage , the use of Braf as a biomarker for detecting microwave radiation-induced brain damage, kits, the use of reagents for specifically detecting Braf in the preparation of kits, the use of Braf function promoters in the preparation of medicines, and the detection of microwave radiation-induced brain damage Methods. Background technique [0002] Microwave technology is widely used in communication, medical treatment, industry, military, etc. Microwave radiation damage has become a hot issue of social concern. Studies have shown that the brain is a sensitive target organ for microwave radiation damage, and the hippocampus is an importan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883G01N33/68A61K45/00A61P25/00
CPCC12Q1/6883G01N33/6893A61K45/00A61P25/00C12Q2600/158G01N2333/47G01N2800/28
Inventor 彭瑞云谭胜芝王惠徐新萍喻超郝延辉赵黎张静王浩宇董霁姚斌伟高亚兵
Owner ACADEMY OF MILITARY MEDICAL SCI
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