A Bacteriostatic Experimental Method for Determining the Molecular Weight of Antibacterial Protein Fragments

Abstract

An antibacterial experiment method capable of determining the molecular weight of antibacterial protein fragments, which relates to the field of microbiological technology; after preparing reagents, separating gels and stacking gels, protein electrophoresis is performed on the proteins subjected to antibacterial experiments, and the obtained protein electrophoresis films are decolorized and stained. After the molecular weight of the protein was observed by imaging, it was used for antibacterial experiments; the protein electrophoresis film was placed in PBS buffer for 10 minutes, and then placed in an agar dish; the bacterial solution in the logarithmic growth phase was added to a 50°C In the nutrient agar medium, obtain a bacterial solution with a volume concentration of 1%; take the obtained bacterial solution and pour it into a petri dish with decolorized protein electrophoresis film, observe the growth of the bacterial cells in the medium, and confirm that the antibacterial effect is good The molecular weight of protein fragments. The invention adopts a simple, accurate, fast and cheap method to determine the molecular weight of protein fragments with antibacterial effect, and has broad application prospects.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to an antibacterial experimental method capable of determining the molecular weight of antibacterial protein fragments. Background technique [0002] The tests used to determine the effectiveness of antibacterial drugs in inhibiting bacterial growth in vitro are called bacteriostatic tests. Through the antibacterial experiment, the minimum inhibitory concentration of a drug can be determined to evaluate the antibacterial performance of the drug, which is the most basic pharmacodynamic data of antibacterial drugs. The main methods are diffusion method for qualitative determination (such as plaque inhibition test) and dilution method for quantitative determination (such as minimum inhibitory concentration test). Commonly used methods are constant broth dilution method, micro broth dilution method, agar dilution method, disc diffusion method and E test (concentration gradient a...

AI Technical Summary

Problems solved by technology

However, the current antibacterial zone method cannot determine the fragment size of the protein with antibacterial effect, and there are more inconveniences in later experiments. It is necessary to conduct antibacterial tests on proteins of each fragment size, and then purify the target protein, which requires a lot of work. It is extremely large, and the presence of miscellaneous proteins also has a certain impact on the test results, affecting the accuracy of the test data

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