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Method adopting tannin to process silkworm cocoon processing wastewater and recycle sericin protein

A technology for sericin and wastewater processing, which is applied in peptide preparation methods, chemical instruments and methods, flocculation/precipitation water/sewage treatment, etc., and can solve the problems of low sericin recovery rate, low treatment efficiency, and high operating costs, etc. problems, to achieve the effects of fast and efficient action, high COD removal rate, and simple separation process

Inactive Publication Date: 2009-11-11
GUILIN AONISITE ENERGY SAVING & ENVIRONMENT PROTECTION TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing methods such as acid analysis method, chemical coagulation method, etc., these methods have defects such as low processing efficiency, high operating cost, low recovery rate of sericin protein and many impurities

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The present invention utilizes the coagulation effect of tannin on protein to achieve the purpose of reclaiming sericin protein from sericin wastewater and treating wastewater. The process steps are as follows:

[0022] (1) Prepare organic solvent solutions such as water or ethanol containing tannin 20g / L with tannin extract;

[0023] (2) Add certain concentration of sulfuric acid or hydrochloric acid under stirring conditions to the sericin-containing waste water, so that the pH value of the waste water is about 4;

[0024] (3) Add the tannin solution under sufficient stirring until the waste water is clarified and the sericin flocculation and precipitation are complete;

[0025] (4) leave standstill, make solid-liquid separation with known methods such as sedimentation, filtration, centrifugation;

[0026] (5) After the effluent of step (4) is further purified by sand filtration, it will be discharged or reused when it reaches the second-level discharge standard;

...

Embodiment 2

[0029] (1) prepare an aqueous solution containing tannin 8g / L with tannin extract;

[0030] (2) In the cocoon-boiling wastewater containing sericin, add a certain concentration of sulfuric acid or hydrochloric acid under stirring conditions, so that the pH value of the wastewater is about 5;

[0031] (3) Add the tannin solution while fully stirring until the waste water is clarified and the sericin flocculation and precipitation are complete;

[0032] All the other are with embodiment 1. The waste water shall be discharged or reused if it meets the secondary discharge standard.

Embodiment 3

[0034] (1) Prepare an aqueous solution containing tannin 1g / L with tannin extract;

[0035] (2) In the silk reeling wastewater containing sericin, add a certain concentration of sulfuric acid or hydrochloric acid under stirring conditions, so that the pH value of the wastewater is about 5;

[0036] (3) Add the tannin solution while fully stirring until the waste water is clarified and the sericin flocculation and precipitation are complete;

[0037] All the other are with embodiment 1. The waste water shall be discharged or reused if it meets the secondary discharge standard.

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PUM

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Abstract

The invention discloses a method which adopts tannin to process silkworm cocoon processing wastewater and recycle sericin protein. The method is characterized in that: firstly, tannin solution is prepared by a conventional method; secondly, under the condition of stirring, sulphuric acid or hydrochloric acid is added to wastewater containing sericin; thirdly, under the condition of complete stirring, the tannin solution is added until the sericin protein is completely precipitated, and the turbid wastewater can be clarified; fourthly, standing is carried out for solid-liquid separation; fifthly, after sand in water is filtered, the wastewater reaches the grade 2 discharge standard and then is discharged or recycled; and sixthly, solid substances are cleaned and treated by alcohol to causethe solid-liquid separation, and after the solid is dried the sericin protein can be obtained. The method has the advantages that the tannin is the pure natural and environment-friendly protein flocculant and is available; tannin can fast and efficiently act with the sericin protein, the sericin protein can be separated out more completely than other methods, the formed floccule is easy to precipitate, the separation technology is simple, and the COD removal rate is high.

Description

technical field [0001] The invention relates to the treatment of organic waste water, in particular to a method for treating cocoon processing waste water with tannin and recovering sericin. Background technique [0002] Silkworm cocoon processing produces high-concentration organic wastewater containing a large amount of sericin. How to effectively treat such wastewater and recycle sericin, a natural resource, to turn waste into treasure is an urgent problem for the silkworm cocoon processing industry. Existing methods, such as acid analysis method, chemical coagulation method, etc., have defects such as low processing efficiency, high operating cost, low recovery rate of sericin protein and many impurities. Contents of the invention [0003] The purpose of the present invention is to disclose a method for treating silkworm cocoon processing sericin waste water and recovering sericin protein with tannin, which is low in cost, high in efficiency, simple in process, easy in...

Claims

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Application Information

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IPC IPC(8): C07K14/435C07K1/30C02F1/54
Inventor 何星存
Owner GUILIN AONISITE ENERGY SAVING & ENVIRONMENT PROTECTION TECH
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