Method for preparing layer-by-layer self-assembled protein-imprinted polymer of chitosan

A technology of western imprinting and layer-by-layer self-assembly, which is applied in the fields of polymer chemistry and protein selective adsorption and separation, can solve the problems of complex preparation process, protein denaturation and inactivation, and achieve simple preparation method, reduced denaturation, and good protein selection The effect of adsorption and separation functions
CN101787143AInactive Publication Date: 2010-07-28WUHAN UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
WUHAN UNIV OF TECH
Publication Date
2010-07-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for preparing layer-by-layer self-assembled protein-imprinted polymer of chitosan. A cross-linked chitosan spherical particle substrate is first prepared, electronegative template protein BSA and electropositive chitosan are alternately adsorbed by the method of layer-by-layer self-assembly, that is, the template protein and the chitosan are alternately electrostatically self-assembled on the surface of the cross-linked chitosan spherical particle substrate, the imprinting of template protein molecules is carried out, eluent is then adopted to remove the template protein molecules, so that a site and hole structure which can identify the template protein and vice versa is left on the surface of the substrate, consequently, the substrate has the selective adsorption and separation functions of the protein, and the layer-by-layer self-assembled protein-imprinted polymer of chitosan is obtained by the method. The polymer prepared by the method has good capability to selectively adsorb the template protein, and has a good selective protein separation function, meanwhile, the preparation method is simple, preparation conditions are mild, and the method has good recyclability.
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Description

technical field

[0001] The invention belongs to the fields of macromolecular chemistry and protein selective adsorption and separation, in particular to a preparation method of chitosan layer-by-layer self-assembled protein imprinted polymer with protein selective adsorption and separation functions. Background technique

[0002] At present, the efficient and selective adsorption separation of biological macromolecules, especially protein molecules, is a challenging research field that has attracted great attention from researchers. In recent years, the protein molecular imprinting method has become a novel and effective protein selective adsorption separation technology, showing important application value and broad development prospects. At present, molecular imprinting techniques are used to separate proteins mainly include: embedding method, surface imprinting method and epitope method [Chemical Bulletin, 2005, 7: 504-509]. However, there are still some problems in thes...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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