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Method for improving solubility of zein through subcritical water modification

A zein, subcritical water technology, applied in the directions of plant zein, chemical instruments and methods, specific peptides, etc., can solve the problems of difficult absorption and utilization, low nutritional value, and difficulty, and achieves the improvement of space. Low utilization rate, equipment cost and operating cost, and the effect of reducing usage and energy consumption

Inactive Publication Date: 2017-10-27
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, protein contains a large number of non-polar amino acids, so it is difficult to dissolve in water
Moreover, zein also lacks the essential amino acids tryptophan and lysine in the human body, and its nutritional value is extremely low, so it is difficult to be absorbed and utilized by organisms

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take 1g of zein, pump 30ml of deionized water into the subcritical reactor, control the pressure in the subcritical reactor to 4MPa, and the reaction temperature to 110°C, react under subcritical water for 20min, pour out the reaction solution, cool down. Then add the same volume of deionized water, react under the same subcritical water conditions for 20min, pour out the reaction solution and mix it with the reaction solution poured out for the first time, and concentrate the mixed solution under reduced pressure in a rotary evaporator. The solution was mixed with the insoluble protein, and the sample was freeze-dried in a freeze dryer into a powder for later use. Dissolve 0.5 g of corn flour in 30 ml of deionized water, centrifuge at 5000 r / min for 10 min to obtain the supernatant, and measure its solubility to 366.56 μg / mL by Folin’s phenol method.

Embodiment 2

[0024] Take 1g of zein, pump 30ml of deionized water into the subcritical reactor, control the pressure in the subcritical reactor to 20MPa, and the reaction temperature to 170°C, react under subcritical water for 120min, pour out the reaction solution, cool down. Then add the same volume of deionized water, react for 120min under the same subcritical water condition, pour out the reaction solution and mix it with the reaction solution poured out for the first time, and concentrate the mixed solution under reduced pressure in a rotary evaporator. The solution was mixed with the insoluble protein, and the sample was freeze-dried in a freeze dryer into a powder for later use. Dissolve 0.5 g of corn flour in 40 ml of deionized water, centrifuge at 5000 r / min for 10 min to get the supernatant, and measure its solubility to 666.23 μg / mL by Folin’s phenol method.

Embodiment 3

[0026] Take 1g of zein, pump 50ml of deionized water into the subcritical reactor, control the pressure in the subcritical reactor to 8MPa, and the reaction temperature to 120°C, react under subcritical water for 20min, pour out the reaction solution, cool down. Then add the same volume of deionized water, react under the same subcritical water conditions for 20min, pour out the reaction solution and mix it with the reaction solution poured out for the first time, and concentrate the mixed solution under reduced pressure in a rotary evaporator. The solution was mixed with the insoluble protein, and the sample was freeze-dried in a freeze dryer into a powder for later use. Dissolve 0.5g of corn flour in 30ml of deionized water, centrifuge at 5000r / min for 10min to get the supernatant, and measure its solubility to 408.76μg / mL by Folin’s phenol method.

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Abstract

The invention discloses a method for improving the solubility of zein through subcritical water modification, and belongs to the technical field of food / agricultural products and by-products thereof. The method comprises the following specific steps of subcritical water modification, filtration, vacuum concentration and freeze drying. The dissolution characteristic of the obtained zein is superior to that of untreated zein. Treatment of a water-insoluble protein is carried out by adopting the green, safe and environment-friendly technical means of subcritical water modification, so that the water-insoluble protein is transformed into a soluble protein, and the method is an environment-friendly protein-modifying technique. The method is suitable for research on improvement of the solubility of the protein in all raw materials.

Description

technical field [0001] The invention belongs to the technical field of processing food / agricultural products and their by-products, and relates to a method for improving the solubility of water-insoluble proteins, in particular to a method for subcritical water modification to improve the solubility of zein. Background technique [0002] Corn is one of the three major food crops in the world. It has the advantages of high yield per unit area and huge potential for increasing production. It occupies an important position in my country's agricultural production. my country's total corn production ranks second in the world, accounting for about 20% of the world's total corn production. However, the development of the processing industry corresponding to the huge output is extremely slow. Corn is mainly used to prepare starch, and 40-50% of the corn raw material is not fully utilized. [0003] In the dry weight of corn, in addition to starch, it actually contains 6-12% (dry wei...

Claims

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Application Information

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IPC IPC(8): C07K14/425
CPCC07K14/425
Inventor 段玉清张继贤张海晖文超婷罗孝平张迪何远清马海乐
Owner JIANGSU UNIV
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