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Method of hydrolyzing protein through enzyme

A protein and enzymatic hydrolysis technology, applied in the field of enzymatic protein hydrolysis, can solve the problems of easy loss of autolytic hydrolase activity, easy inactivation of autolytic hydrolase, long reaction time, etc. The effect of production costs

Inactive Publication Date: 2005-08-31
SICHUAN NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many kinds of hydrolytic enzymes in the cells and tissues, and their activities are different, and they will also be affected by other substances in the body. The activity of autolytic hydrolytic enzymes in the body is easy to lose
Moreover, using the autolyzed hydrolase present in the body to hydrolyze protein has the disadvantages of long reaction time, low efficiency, low yield, and easy inactivation of the autolyzed hydrolase.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Wash the earthworms, spit out sand, remove impurities, and evenly crush them. Take 0.5 grams of earthworms, add 2.5 mg of neutral protease into a reaction vessel with 50 ml of buffer solution, shake, heat to make the reaction temperature 50°C, hydrolase The amount of enzyme added is 15000U / g, the mass fraction of protein is 1.0%, the pH value in the reaction system is 7.0, and the reaction time is 5 hours. After fully reacting, use the ninhydrin chromogenic method to measure the amino acid content on a spectrophotometer, and the hydrolysis rate can reach more than 65.98%.

Embodiment 2

[0031] Wash the earthworms, spit out sand, remove impurities, and evenly crush them. Take 15 grams of earthworms, add 30 milligrams of alkaline protease to a reaction vessel of 1000 milliliters of buffer solution, heat to make the reaction temperature 50 ° C, the amount of hydrolytic enzyme added is 6000 U / g, and the mass fraction of protein is 1.5%, the reaction system The pH value in the medium is 8.4, and the reaction time is 5 hours. After fully reacting, use the ninhydrin chromogenic method to measure the amino acid content on a spectrophotometer, and the hydrolysis rate can reach more than 48.49%.

Embodiment 3

[0033] Wash the earthworms, spit out sand, remove impurities, and evenly crush them. Take 0.5 grams of earthworms, mix 5 mg of compound protease, that is, neutral protease and alkaline protease, wherein 4 mg of neutral protease and 1 mg of alkaline protease are added to a reaction vessel with 50 ml of buffer solution , heated so that the reaction temperature is 50°C, the amount of compound protease added is 30000U / g, the protein mass fraction is 1.0%, the pH value in the reaction system is 7.2, and the reaction time is 5 hours. After fully reacting, use the ninhydrin chromogenic method to measure the amino acid content on a spectrophotometer, and the hydrolysis rate can reach more than 58.76%.

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Abstract

A process for enzymolyzing the protein to obtain the high-activity enzymolyzed liquid containing amino acids, micro-molecular peptide, nucleotide, glycerine and fatty acids includes washing earthworm or gains for removing impurities, proportionally mixing it with neutral proteinase and / or alkaline proteinase, adding buffer liquid to regulate pH value, and reacting at 20-75 deg.C for at least 2 hrs.

Description

1. Technical field [0001] The invention relates to a technology of enzymatic hydrolysis of protein, more specifically to a method for enzymatic hydrolysis of protein with high activity enzymatic hydrolyzate obtained from animal body or grain protein. 2. Background technology [0002] At present, there are mainly three methods used in protein hydrolysis at home and abroad: acid hydrolysis, alkali hydrolysis and enzyme hydrolysis. Among them, acid hydrolysis and alkali hydrolysis belong to chemical hydrolysis, while enzymatic hydrolysis belongs to biological enzyme hydrolysis. The acid hydrolysis method is usually hydrolyzed with a strong acid, namely sulfuric acid or hydrochloric acid. The molar concentration of the acid is 6 mol / liter, and the hydrolysis is carried out for more than 20 hours at a high temperature of 110°C. Afterwards, asparagine and glutamine were completely hydrolyzed into aspartic acid and glutamic acid, respectively, and tryptophan was completely destroy...

Claims

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Application Information

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IPC IPC(8): C12P21/00
Inventor 赵仕林杨丽雪王锡宇廖洋
Owner SICHUAN NORMAL UNIVERSITY
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