Kit for detecting polymorphism of non-small cell lung cancer mononucleotide and application of kit

Abstract

The invention discloses a kit for detecting polymorphism of non-small cell lung cancer mononucleotide. The kit comprises PCR reaction reagents, specific primers and single-base extension primers, wherein the specific primers and the single-base extension primers are used for genetic locus SNP detection, and genetic loci comprise the CBR1 genetic locus rs3787728, the TP63 genetic locus rs7631358 and the CIR1 genetic locus rs13009079. The reaction system can detect multiple genetic polymorphic loci, decrease the reaction frequency, increase the detection throughput and reduce usage of samples and various consumables; reagents such as fluorescent dye and special enzymes which are high in price are not needed, and the cost is low; the accuracy is 99.99%, the sensitivity is 5 ng of genome DNA, and the kit can accurately detect out polymorphic loci of multiple genes and has the advantages of being high in throughput, automatic, low in cost and the like.

Description

(1) Technical field [0001] The invention relates to a single nucleotide polymorphism detection kit, in particular to a non-small cell lung cancer single nucleotide polymorphism detection kit and application thereof. (2) Background technology [0002] Lung cancer is one of the most common malignant tumors, and its morbidity and mortality continue to rise, seriously threatening human health and life. Non-small cell lung cancer (NSCLC) accounts for the vast majority of pathological types of lung cancer. Lung cancer is a multifactorial and polygenic disease caused by both environmental and genetic factors. With the development of molecular medicine, a large number of genes related to lung cancer have been discovered, but the pathogenesis of lung cancer is still not completely clear, and the problems of early diagnosis and prevention of lung cancer are still not fully resolved. Therefore, the control of variable factors, such as the prevention of lung cancer risk factors, can d...

AI Technical Summary

Problems solved by technology

With the development of molecular medicine, a large number of lung cancer-related genes have been discovered, but the pathogenesis of lung cancer is still not completely clear, and the early diagnosis and prevention of lung cancer are still not fully resolved

Therefore, the control of variable factors, such as the prevention of lung cancer risk factors, can delay and prevent the onset of lung cancer to a certain extent, but the lack of understanding of fixed genetic factors seriously affects the prognosis of lung cancer to a certain extent. Onset, diagnosis and treatment

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