Kit for detecting polymorphism of non-small cell lung cancer mononucleotide and application of kit

A single nucleotide polymorphism, non-small cell lung cancer technology, applied in the determination/test of microorganisms, biochemical equipment and methods, etc., can solve the problem of early diagnosis and prevention of lung cancer, which cannot be completely solved, and affects the incidence and diagnosis of lung cancer. and treatment, the pathogenesis of lung cancer is not completely clear and other issues, to achieve the effect of low cost, reduce the number of reactions, and reduce costs

Inactive Publication Date: 2016-11-09
ZHEJIANG PROVINCIAL HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

With the development of molecular medicine, a large number of lung cancer-related genes have been discovered, but the pathogenesis of lung cancer is still not completely clear, and the early diagnosis and prevention of lung cancer are still not fully resolved
Therefore, the control of var

Method used

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  • Kit for detecting polymorphism of non-small cell lung cancer mononucleotide and application of kit
  • Kit for detecting polymorphism of non-small cell lung cancer mononucleotide and application of kit
  • Kit for detecting polymorphism of non-small cell lung cancer mononucleotide and application of kit

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Embodiment 1

[0038] Kit composition: H 2 O, containing 15mM MgCl 2 10×PCR Buffer 0.625μL, 25mM MgCl 2 0.325μL, 2.5mM dNTP 1μL, gene locus SNP detection specific primer 1μL, 5U / μL HotstarTaq 0.1μL, 10×SAPBuffer 0.17μL, 1U / μL SAP enzyme 0.3μL, 10×iPlex Buffer 0.2μL, iPlex Termination mix0 .2 μL, site-specific extension primer 0.804 μL, iPlex enzyme 0.041 μL.

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Abstract

The invention discloses a kit for detecting polymorphism of non-small cell lung cancer mononucleotide. The kit comprises PCR reaction reagents, specific primers and single-base extension primers, wherein the specific primers and the single-base extension primers are used for genetic locus SNP detection, and genetic loci comprise the CBR1 genetic locus rs3787728, the TP63 genetic locus rs7631358 and the CIR1 genetic locus rs13009079. The reaction system can detect multiple genetic polymorphic loci, decrease the reaction frequency, increase the detection throughput and reduce usage of samples and various consumables; reagents such as fluorescent dye and special enzymes which are high in price are not needed, and the cost is low; the accuracy is 99.99%, the sensitivity is 5 ng of genome DNA, and the kit can accurately detect out polymorphic loci of multiple genes and has the advantages of being high in throughput, automatic, low in cost and the like.

Description

(1) Technical field [0001] The invention relates to a single nucleotide polymorphism detection kit, in particular to a non-small cell lung cancer single nucleotide polymorphism detection kit and application thereof. (2) Background technology [0002] Lung cancer is one of the most common malignant tumors, and its morbidity and mortality continue to rise, seriously threatening human health and life. Non-small cell lung cancer (NSCLC) accounts for the vast majority of pathological types of lung cancer. Lung cancer is a multifactorial and polygenic disease caused by both environmental and genetic factors. With the development of molecular medicine, a large number of genes related to lung cancer have been discovered, but the pathogenesis of lung cancer is still not completely clear, and the problems of early diagnosis and prevention of lung cancer are still not fully resolved. Therefore, the control of variable factors, such as the prevention of lung cancer risk factors, can d...

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q1/6858C12Q2600/156C12Q2600/16C12Q2531/113C12Q2533/101
Inventor 谷建钟袁凯施吴建浓张碧燕沈莹莹李妍姜贻乾朱影王赟周华妙丁霞朱慧萍张永军郭勇
Owner ZHEJIANG PROVINCIAL HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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