34 results about "Microchip Electrophoresis" patented technology

The present invention relates to a microchip apparatus using liquids. More specifically, the invention provides a liquid mixing apparatus comprising at least two microchannels for introducing liquids and a mixing microchannel that connects to the at least two liquid-introducing microchannels, wherein the liquids are transported from the respective liquid-introducing microchannels toward the mixing microchannel, the apparatus further comprising means for enhancing the mixing of the liquids that converge in the mixing microchannel. The invention also provides an electrophoretic apparatus and a microchip electrophoretic apparatus for denaturing gradient gel electrophoresis.

A microchip electrophoresis device is provided with: a sample setting unit for setting at least one sample and at least one standard sample; at least one microchip having a flow path for electrophoresis therein; an introduction device that introduces the separation buffer, the sample, and the standard sample into the flow path; a voltage applying device that applies a voltage to the flow path; a detection unit that acquires analysis data for electrophoretic analysis in the flow path; and a controller that controls the operation of the introduction device and the voltage application device, the controller being provided with: a standard data storage unit that stores standard data obtained by performing electrophoretic analysis on the standard sample under predetermined conditions; and a chip determination unit that performs chip determination before performing electrophoretic analysis on the sample by performing electrophoretic analysis on the standard sample using each microchip under predetermined conditions, acquiring analysis data of the standard sample by the detection unit, and comparing the acquired analysis data with standard data of the standard sample, it is determined whether or not the state of each microchip is suitable for electrophoretic analysis.

Devices and methods are provided for the separation and dispensing of material using a microfluidic electrophoresis column, sheath liquid pump, and exit channel, all on the same monolithic chip. Material is separated in the electrophoresis column and passed into the exit chamber in response to a voltage potential between a first electrode within the electrophoresis column and a terminating electrode integrated into the chip. The terminating electrode can be in the sheath liquid pump chamber, the sheath liquid reservoir, or a separate flow channel that intersects the exit channel along with the electrophoresis column and sheath liquid pump chamber. The flow of sheath liquid into the exit chamber entrains separated analytes into an effluent that is dispensed out of the exit chamber via a discharge outlet.

A separation carrier comprising β-1,3-glucan and / or methyl cellulose; a running buffer comprising the separation carrier; a method of capillary electrophoresis or microchip electrophoresis, wherein a sample comprising macromolecular compounds is run in the presence of the running buffer; a method of capillary electrophoresis or microchip electrophoresis, comprising the step of injecting the sample by an electrical injection or application of pressure; and a method for analyzing macromolecular compounds by the method of capillary electrophoresis or microchip electrophoresis. According to the present invention, it is possible to achieve high resolution quickly. Therefore, the methods are useful in the High Through-put screening analysis of proteins or sugar chains in gene analysis, proteome analysis or glycome analysis, and applicable in medical diagnostic apparatuses and the elucidation of biological functions, mechanisms of onset of diseases, etc.

A microchip electrophoresis apparatus includes a microchip, a dispensing part, a suction part, and a control part. The control part is configured to perform a buffer solution filling step and a liquid surface aligning step. In the buffer solution filling step, a buffer solution is filled in a channel of the microchip, and also a liquid surface level of the buffer solution in a first reservoir and a liquid surface level of the buffer solution in a second reservoir provided on the other end of the channel are set to a predetermined level or more. The liquid surface aligning step is performed after the buffer solution filling step. In the liquid surface aligning step, tips of a first suction nozzle and a second suction nozzle are lowered from above the first reservoir and the second reservoir to the predetermined level while allowing the first suction nozzle and the second suction nozzle to perform suction operation, such that the buffer solution in the first reservoir and the second reservoir is sucked in order from a surface layer side, and aligning the liquid surface level of the first reservoir with the liquid surface level of the second reservoir.