31 results about "Microchip Electrophoresis" patented technology

The present invention relates to a microchip apparatus using liquids. More specifically, the invention provides a liquid mixing apparatus comprising at least two microchannels for introducing liquids and a mixing microchannel that connects to the at least two liquid-introducing microchannels, wherein the liquids are transported from the respective liquid-introducing microchannels toward the mixing microchannel, the apparatus further comprising means for enhancing the mixing of the liquids that converge in the mixing microchannel. The invention also provides an electrophoretic apparatus and a microchip electrophoretic apparatus for denaturing gradient gel electrophoresis.

The invention discloses a manufacture method of a detection electrode of a micro-fluidic chip and a preparation method of an electrophoresis non-contact type conductivity detection system. The manufacture method of the compound mold of the micro-fluidic chip comprises the steps of adhering and pressing a sensitive dry film on a substrate under a non-exposed condition, exposing and developing. The preparation method of the detection electrode comprises the steps of adhering and pressing a light-sensitive material on a conductive film layer under the non-exposed condition; exposing; developing; etching; and removing the light-sensitive material. By adopting the manufacture and preparation methods, the compound mold and the corresponding micro-fluidic chip, conductive film electrode and micro-fluidic chip electrophoresis non-contact type conductivity detection system can be obtained in a quick and economical mode.

A pretreatment method of samples, in which injections of samples are performed efficiently and precisely when amino acids are analyzed with a mass spectrometer, is provided. For the analysis method of samples including analyte comprising an amino acid, an amine and/or a peptide with mass spectrometry, the analyte is derivatized with a modification reagent, the derivative is subjected to a microchip electrophoresis, and then eluate from the microchip electrophoresis is introduced into a mass spectrometer.

An separation method comprising a temperature control process useful for microchip electrophoresis such as microchip DGGE is provided along with a device therefor.

The present invention relates to a microchip electrophoresis method for separating double-stranded nucleic acids by means of differences in nucleotide sequence while maintaining a preset temperature, wherein the temperature during separation of double-stranded nucleic acids in an separation region comprising an separation microchannel is controlled to within ±2.5° C. of the preset temperature.

To provide a method for analyzing a nucleic acid according to high throughput microcapillary electrophoresis during microchip electrophoresis under non-steady electric field, the method being capable of detecting polymorphism of a large number of genes at a high speed. The present invention is useful for the diagnosis and treatment of diseases such as detection of gene diseases and application to Taylor-made therapy.

The invention relates to a microchip electrophoresis system capable of synchronously detecting negative and positive ions and a detection method thereof. The system comprises a micro-fluidic chip, a high-voltage generation control module, a non-contact conductance detection module and a control module. The micro-fluidic chip comprises a microchip cover plate, an insulating layer and a PCB substrate. A micro pipeline is arranged at the bottom of the microchip cover plate. A transmitting electrode, a receiving electrode and a ground electrode are etched on the top of the PCB substrate. The micropipeline comprises a first channel and a second channel which are arranged in a crossed mode and communicated with each other. The two ends of the first channel are provided with a sample reservoir and a sample waste liquid tank respectively. The two ends of the second channel are provided with a buffer solution reservoir and a buffer solution waste liquid tank respectively. The first channel isin a linear shape. The second channel comprises a linear first connecting part, a second connecting part and an arc-shaped third connecting part. According to the system and the method, synchronous detection of positive and negative ions can be realized by only needing the single inlet sample, the micro pipeline and the non-contact conductance detection system.

When the migration time of a low molecular weight compound having an unknown migration time in microchip electrophoresis, capillary electrophoresis, or a capillary electrophoresis mass spectrometer is predicted, first, with respect to a substance having a known electrophoretic migration time, characteristic quantities (descriptors) thereof which can be numerically expressed from a structure thereof are computed to predict the relation between the characteristic quantities (descriptors) and the migration time; the migration times of some substances are measured by electrophoresis or an electrophoresis mass spectrometer to learn about the relation; and using the learnt result, the migration time of the substance having an unknown migration time in the electrophoresis or electrophoresis mass spectrometer is predicted from the structure thereof.

The invention discloses a device for detecting microchip electrophoresis by time and space resolution. The device consists of two parts comprising a separation part and a detection part, wherein the separation part consists of a PDMS (polydimethylsiloxane) microchip, a high voltage power source and a platinum electrode; the detection part consists of an ultraviolet source, a linear light source converter and a liner detector; the ultraviolet source is linearly converted and covers a whole microchip channel; the linear detector of which the size is similar to that of the microchip channel is used for detecting the whole microchip channel; the linear light source converter, the PDMS microchip and the linear detector are integrated to a dimming light path; optical calibration is performed prior to each time of detection, which is carried out at the position having the strongest signal, and then other parts, such as electrodes, and the like, are connected to a system; and after the detection at each time, relevant data processing is performed according to the requirement of the time and space resolution detection. The device for detecting microchip electrophoresis by the time and space resolution has high time and space resolution, is particularly suitable for representing interaction of biological molecules, and can be used for isoelectrofocusing analysis of protein, and the like.

The invention relates to a system and a method for synchronous detection and separation of negative and positive ions based on microchip electrophoresis. The system comprises a micro-fluidic chip, a high-voltage generation control module, a non-contact conductance detection module and a control module. The micro-fluidic chip comprises a microchip cover plate, an insulating layer and a PCB substrate. A micro pipeline is arranged at the bottom of the microchip cover plate. A detection electrode is etched on the top of the PCB substrate. The micro pipeline comprises a linear first channel and a Y-shaped second channel. A sample reservoir and a sample waste liquid tank are arranged at the two ends of the first channel respectively. The second channel comprises a first connecting part, and a second connecting part and a third connecting part which are symmetrically arranged on the front side and the rear side of the tail end of the first connecting part. A buffer solution reservoir is arranged at the head end of the first connecting part; a positive ion reservoir is arranged at the tail end of the second connecting part; and a negative ion reservoir is arranged at the tail end of the third connecting part. According to the system and the method, synchronous detection and separation of positive and negative ions can be realized by only needing the single inlet sample, the micro pipeline and the non-contact conductance detection module.

The invention discloses a photosensitive compound and a preparation method and application thereof, and relates to the technical field of protein nucleic acid co-immobilization, the photosensitive compound is a polyacrylamide compound, and comprises a tetrazole group and a furan group; the compound is prepared from the following raw materials: N-(3-aminopropyl) tert-butyl carbamate, methacryloyl chloride furan-2-yl boric acid, [hydroxyl (phenyl)-lambda 3-iodo] 4-methyl benzene sulfonate and the like. Protein and nucleic acid co-immobilization electrophoresis gel can be prepared, and protein and nucleic acid in single cells or multiple cells can be quantitatively or semi-quantitatively detected; the compound can also be used for microchip electrophoresis mobility analysis, western blotting, nucleic acid blotting hybridization, single-cell western blotting, single-cell northern/source blotting hybridization or capillary electrophoresis western blotting. The protein and nucleic acid co-immobilization gel can be used for simultaneously immobilizing protein and nucleic acid in situ to research the interaction between the protein and DNA (Deoxyribose Nucleic Acid).

A sample, which is a mixture of glycans, is fluorescently labeled (S2). The sample is subsequently separated by microchip electrophoresis under a buffer solution with no lectin added as well as under multiple kinds of buffer solutions with different lectins respectively added, and the separated components are fluorescently detected (S3). A high-concentration gel which can produce a molecular-sieving effect is used as the buffer solution. Multiple electropherograms are created from the detection results (S4). A glycan having a lectin specifically attached is delayed during its migration in the buffer solution, so that a peak corresponding to this glycan will effectively disappear. Accordingly, based on the kinds of lectins and the presence/absence of a peak on each of the electropherograms, the structure of each glycan in the sample is estimated and the glycan is identified (S5).

The invention provides devices and methods for the detection of hydrophobic biomarkers using 3D microchip capillary electrophoresis having a non-aqueous solvent system. Hydrophobic biomarkers can be placed in a microcapillary microchannel and electrokinetically injected into a second microcapillary microchannel through a nanocapillary array membrane. The hydrophobic biomarkers can then be separated and analyzed via mass spectrometry. Certain hydrophobic biomarkers can indicate a particular disease state.

A microchip electrophoresis method includes sequentially analyzing a plurality of samples by repeating an analysis process by electrophoresis separation in a microchip, executing a cleaning process of the microchip, and setting timing of the cleaning process of the microchip at arbitrary timing between a plurality of analysis processes.

By using an electrophoretic buffer comprising a polymerized polymer micelle formed by the steps comprising dispersing into an aqueous medium a block copolymer represented by HPLS-HPBS-PLZA, wherein HPLS is a hydrophilic polymer segment, HPBS is a hydrophobic polymer segment, and PLZA is a polymerizable group having an ethylenically unsaturated double bond, and polymerizing the block copolymer as a buffer for a capillary electrophoresis or a microchip electrophoresis, pressurizing the sample after introduction at a given pressure for a given time period, and electrophoresing in an electrophoretic electric field, a polymer compound such as DNA can be separated rapidly and in high separation ability.

A sample setting section in which at least one sample to be subjected to electrophoresis analysis and at least one standard sample are set, and a controller. The controller includes a standard data storage memory that stores standard data obtained by performing electrophoresis analysis of a standard sample under a predetermined condition, and a chip determination part configured to perform electrophoresis analysis of the standard sample under the predetermined condition using each of the at least one microchip before electrophoresis analysis of the sample set in the sample setting section is performed to acquire analysis data on the standard sample by the detector, and to perform a chip determination as to whether a state of each of the at least one microchip is suitable for performing electrophoresis analysis by comparing the acquired analysis data with the standard data on the standard sample.