The invention relates to the technical field of food detection, and discloses a primer combination, a method and a kit for synchronously detecting 14 kinds of animal-derived components in meat or meat products. The present invention uses 10 pairs of specific primers to identify whether pigs, cattle, sheep, goats, chickens, ducks, dogs, foxes, raccoon dogs, cats, and mice are contained in meat or meat products based on two 5-fold PCR reactions and microchip electrophoresis technology. , donkey, deer and horse 14 animal-derived components, the sequences of the 10 pairs of specific primers are shown in SEQ ID NO.1-20. The method of the present invention has the advantages of accurate detection, high sensitivity, strong specificity, simplicity, rapidity and high efficiency, and can satisfy large-scale and rapid identification of whether pigs, cattle, sheep, goats, chickens, ducks, dogs, foxes, etc. are contained in meat or meat products. Requirements for ingredients derived from raccoon dogs, cats, rats, donkeys, deer and horses can effectively resist the adulteration of meat products, effectively protect the interests of consumers, and have good social benefits.