31 results about "Microchip Electrophoresis" patented technology

To provide a method for analyzing a nucleic acid according to high throughput microcapillary electrophoresis during microchip electrophoresis under non-steady electric field, the method being capable of detecting polymorphism of a large number of genes at a high speed. The present invention is useful for the diagnosis and treatment of diseases such as detection of gene diseases and application to Taylor-made therapy.

The present invention relates to a microchip apparatus using liquids. More specifically, the invention provides a liquid mixing apparatus comprising at least two microchannels for introducing liquids and a mixing microchannel that connects to the at least two liquid-introducing microchannels, wherein the liquids are transported from the respective liquid-introducing microchannels toward the mixing microchannel, the apparatus further comprising means for enhancing the mixing of the liquids that converge in the mixing microchannel. The invention also provides an electrophoretic apparatus and a microchip electrophoretic apparatus for denaturing gradient gel electrophoresis.

By using an electrophoretic buffer comprising a polymerized polymer micelle formed by the steps comprising dispersing into an aqueous medium a block copolymer represented by HPLS-HPBS-PLZA, wherein HPLS is a hydrophilic polymer segment, HPBS is a hydrophobic polymer segment, and PLZA is a polymerizable group having an ethylenically unsaturated double bond, and polymerizing the block copolymer as a buffer for a capillary electrophoresis or a microchip electrophoresis, pressurizing the sample after introduction at a given pressure for a given time period, and electrophoresing in an electrophoretic electric field, a polymer compound such as DNA can be separated rapidly and in high separation ability.

A microchip electrophoresis device is provided with: a sample setting unit for setting at least one sample and at least one standard sample; at least one microchip having a flow path for electrophoresis therein; an introduction device that introduces the separation buffer, the sample, and the standard sample into the flow path; a voltage applying device that applies a voltage to the flow path; a detection unit that acquires analysis data for electrophoretic analysis in the flow path; and a controller that controls the operation of the introduction device and the voltage application device, the controller being provided with: a standard data storage unit that stores standard data obtained by performing electrophoretic analysis on the standard sample under predetermined conditions; and a chip determination unit that performs chip determination before performing electrophoretic analysis on the sample by performing electrophoretic analysis on the standard sample using each microchip under predetermined conditions, acquiring analysis data of the standard sample by the detection unit, and comparing the acquired analysis data with standard data of the standard sample, it is determined whether or not the state of each microchip is suitable for electrophoretic analysis.

Devices and methods are provided for the separation and dispensing of material using a microfluidic electrophoresis column, sheath liquid pump, and exit channel, all on the same monolithic chip. Material is separated in the electrophoresis column and passed into the exit chamber in response to a voltage potential between a first electrode within the electrophoresis column and a terminating electrode integrated into the chip. The terminating electrode can be in the sheath liquid pump chamber, the sheath liquid reservoir, or a separate flow channel that intersects the exit channel along with the electrophoresis column and sheath liquid pump chamber. The flow of sheath liquid into the exit chamber entrains separated analytes into an effluent that is dispensed out of the exit chamber via a discharge outlet.

The invention relates to the technical field of food detection, and discloses a primer combination, a method and a kit for synchronously detecting 14 kinds of animal-derived components in meat or meat products. The present invention uses 10 pairs of specific primers to identify whether pigs, cattle, sheep, goats, chickens, ducks, dogs, foxes, raccoon dogs, cats, and mice are contained in meat or meat products based on two 5-fold PCR reactions and microchip electrophoresis technology. , donkey, deer and horse 14 animal-derived components, the sequences of the 10 pairs of specific primers are shown in SEQ ID NO.1-20. The method of the present invention has the advantages of accurate detection, high sensitivity, strong specificity, simplicity, rapidity and high efficiency, and can satisfy large-scale and rapid identification of whether pigs, cattle, sheep, goats, chickens, ducks, dogs, foxes, etc. are contained in meat or meat products. Requirements for ingredients derived from raccoon dogs, cats, rats, donkeys, deer and horses can effectively resist the adulteration of meat products, effectively protect the interests of consumers, and have good social benefits.

The invention provides a micro-electrophoresis chip and a detection method for online concentration and detection of charged small particles. The micro-electrophoresis chip includes a substrate, three channels are arranged in the substrate, and a plurality of pools are arranged on the substrate, wherein the concentration channel PA, The sample pool P is connected with the concentration pool A; the sampling channel AB is connected with the concentration pool A and the sample waste pool B; the separation channel CD is connected with the buffer pool C and the buffer pool D, and the separation channel CD is connected with the sample injection The channel AB is vertically cross-connected; the above five pools are equipped with high-voltage electrodes for applying high pressure to the pool; the volume of the concentration pool A is adjustable, and the volume of the sample pool P is 10-1000 times the volume of the concentration pool A ; A pair of detection electrodes are provided on the separation channel CD close to the buffer liquid waste pool D, for detecting charged small particles passing through this position. This technology can greatly improve the detection accuracy of the original microchip electrophoresis capacitive coupling non-contact conductometric detection method; while retaining the ME‑C 4 D The advantages of low cost, easy portability, rapid detection, and in-situ detection.

An end of a capillary storing a sample in its interior is inserted into a sample reservoir in a microchip and positioned near an inlet of a channel connecting to the sample reservoir. Electrodes are placed in the other end of the capillary and a waste reservoir. A predetermined voltage is applied between the electrodes so that a potential is applied between the other end of capillary and waste reservoir. As a result, the sample in the capillary is introduced electrophoretically into a separating medium in the channel. When the sample has been introduced into the channel after the lapse of a specified time, the voltage application between the electrodes is stopped and the capillary is pulled out of the sample reservoir. The sample can be introduced into the channel without placing it in the sample reservoir in a filling amount.

A separation carrier comprising β-1,3-glucan and / or methyl cellulose; a running buffer comprising the separation carrier; a method of capillary electrophoresis or microchip electrophoresis, wherein a sample comprising macromolecular compounds is run in the presence of the running buffer; a method of capillary electrophoresis or microchip electrophoresis, comprising the step of injecting the sample by an electrical injection or application of pressure; and a method for analyzing macromolecular compounds by the method of capillary electrophoresis or microchip electrophoresis. According to the present invention, it is possible to achieve high resolution quickly. Therefore, the methods are useful in the High Through-put screening analysis of proteins or sugar chains in gene analysis, proteome analysis or glycome analysis, and applicable in medical diagnostic apparatuses and the elucidation of biological functions, mechanisms of onset of diseases, etc.

A microchip electrophoresis apparatus includes a microchip, a dispensing part, a suction part, and a control part. The control part is configured to perform a buffer solution filling step and a liquid surface aligning step. In the buffer solution filling step, a buffer solution is filled in a channel of the microchip, and also a liquid surface level of the buffer solution in a first reservoir and a liquid surface level of the buffer solution in a second reservoir provided on the other end of the channel are set to a predetermined level or more. The liquid surface aligning step is performed after the buffer solution filling step. In the liquid surface aligning step, tips of a first suction nozzle and a second suction nozzle are lowered from above the first reservoir and the second reservoir to the predetermined level while allowing the first suction nozzle and the second suction nozzle to perform suction operation, such that the buffer solution in the first reservoir and the second reservoir is sucked in order from a surface layer side, and aligning the liquid surface level of the first reservoir with the liquid surface level of the second reservoir.

A sample, which is a mixture of glycans, is fluorescently labeled (S2). The sample is subsequently separated by microchip electrophoresis under a buffer solution with no lectin added as well as under multiple kinds of buffer solutions with different lectins respectively added, and the separated components are fluorescently detected (S3). A high-concentration gel which can produce a molecular-sieving effect is used as the buffer solution. Multiple electropherograms are created from the detection results (S4). A glycan having a lectin specifically attached is delayed during its migration in the buffer solution, so that a peak corresponding to this glycan will effectively disappear. Accordingly, based on the kinds of lectins and the presence / absence of a peak on each of the electropherograms, the structure of each glycan in the sample is estimated and the glycan is identified (S5).