31 results about "Microchip Electrophoresis" patented technology

The present invention relates to a microchip apparatus using liquids. More specifically, the invention provides a liquid mixing apparatus comprising at least two microchannels for introducing liquids and a mixing microchannel that connects to the at least two liquid-introducing microchannels, wherein the liquids are transported from the respective liquid-introducing microchannels toward the mixing microchannel, the apparatus further comprising means for enhancing the mixing of the liquids that converge in the mixing microchannel. The invention also provides an electrophoretic apparatus and a microchip electrophoretic apparatus for denaturing gradient gel electrophoresis.

The present invention provides a microchip for performing electrophoresis with electrochemical means, such as by pulsed amperometric detection (PAD), for the separation and detection of underivatized carbohydrates, amino acids, sulfur-containing antibiotics, etc. The present methods permit the direct detection of amines, thiols, alcohols and carbohydrates and therefore is a useful technique for the development of electrochemical detection for microchip electrophoresis.

The invention discloses a manufacture method of a detection electrode of a micro-fluidic chip and a preparation method of an electrophoresis non-contact type conductivity detection system. The manufacture method of the compound mold of the micro-fluidic chip comprises the steps of adhering and pressing a sensitive dry film on a substrate under a non-exposed condition, exposing and developing. The preparation method of the detection electrode comprises the steps of adhering and pressing a light-sensitive material on a conductive film layer under the non-exposed condition; exposing; developing; etching; and removing the light-sensitive material. By adopting the manufacture and preparation methods, the compound mold and the corresponding micro-fluidic chip, conductive film electrode and micro-fluidic chip electrophoresis non-contact type conductivity detection system can be obtained in a quick and economical mode.

A pretreatment method of samples, in which injections of samples are performed efficiently and precisely when amino acids are analyzed with a mass spectrometer, is provided. For the analysis method of samples including analyte comprising an amino acid, an amine and / or a peptide with mass spectrometry, the analyte is derivatized with a modification reagent, the derivative is subjected to a microchip electrophoresis, and then eluate from the microchip electrophoresis is introduced into a mass spectrometer.

An separation method comprising a temperature control process useful for microchip electrophoresis such as microchip DGGE is provided along with a device therefor.The present invention relates to a microchip electrophoresis method for separating double-stranded nucleic acids by means of differences in nucleotide sequence while maintaining a preset temperature, wherein the temperature during separation of double-stranded nucleic acids in an separation region comprising an separation microchannel is controlled to within ±2.5° C. of the preset temperature.

To provide a method for analyzing a nucleic acid according to high throughput microcapillary electrophoresis during microchip electrophoresis under non-steady electric field, the method being capable of detecting polymorphism of a large number of genes at a high speed. The present invention is useful for the diagnosis and treatment of diseases such as detection of gene diseases and application to Taylor-made therapy.

To provide a method for analyzing a nucleic acid according to high throughput microcapillary electrophoresis during microchip electrophoresis under non-steady electric field, the method being capable of detecting polymorphism of a large number of genes at a high speed. The present invention is useful for the diagnosis and treatment of diseases such as detection of gene diseases and application to Taylor-made therapy.

The present invention provides a microchip for performing electrophoresis with pulsed amperometric detection (PAD) for the separation and detection of underivatized carbohydrates, amino acids, sulfur-containing antibiotics, etc. PAD allows for the direct detection of amines, thiols, alcohols and carbohydrates and therefore is a useful technique for the development of electrochemical detection for microchip electrophoresis.

The invention relates to a microchip electrophoresis system capable of synchronously detecting negative and positive ions and a detection method thereof. The system comprises a micro-fluidic chip, a high-voltage generation control module, a non-contact conductance detection module and a control module. The micro-fluidic chip comprises a microchip cover plate, an insulating layer and a PCB substrate. A micro pipeline is arranged at the bottom of the microchip cover plate. A transmitting electrode, a receiving electrode and a ground electrode are etched on the top of the PCB substrate. The micropipeline comprises a first channel and a second channel which are arranged in a crossed mode and communicated with each other. The two ends of the first channel are provided with a sample reservoir and a sample waste liquid tank respectively. The two ends of the second channel are provided with a buffer solution reservoir and a buffer solution waste liquid tank respectively. The first channel isin a linear shape. The second channel comprises a linear first connecting part, a second connecting part and an arc-shaped third connecting part. According to the system and the method, synchronous detection of positive and negative ions can be realized by only needing the single inlet sample, the micro pipeline and the non-contact conductance detection system.

When the migration time of a low molecular weight compound having an unknown migration time in microchip electrophoresis, capillary electrophoresis, or a capillary electrophoresis mass spectrometer is predicted, first, with respect to a substance having a known electrophoretic migration time, characteristic quantities (descriptors) thereof which can be numerically expressed from a structure thereof are computed to predict the relation between the characteristic quantities (descriptors) and the migration time; the migration times of some substances are measured by electrophoresis or an electrophoresis mass spectrometer to learn about the relation; and using the learnt result, the migration time of the substance having an unknown migration time in the electrophoresis or electrophoresis mass spectrometer is predicted from the structure thereof.

The invention discloses a device for detecting microchip electrophoresis by time and space resolution. The device consists of two parts comprising a separation part and a detection part, wherein the separation part consists of a PDMS (polydimethylsiloxane) microchip, a high voltage power source and a platinum electrode; the detection part consists of an ultraviolet source, a linear light source converter and a liner detector; the ultraviolet source is linearly converted and covers a whole microchip channel; the linear detector of which the size is similar to that of the microchip channel is used for detecting the whole microchip channel; the linear light source converter, the PDMS microchip and the linear detector are integrated to a dimming light path; optical calibration is performed prior to each time of detection, which is carried out at the position having the strongest signal, and then other parts, such as electrodes, and the like, are connected to a system; and after the detection at each time, relevant data processing is performed according to the requirement of the time and space resolution detection. The device for detecting microchip electrophoresis by the time and space resolution has high time and space resolution, is particularly suitable for representing interaction of biological molecules, and can be used for isoelectrofocusing analysis of protein, and the like.

The invention relates to a system and a method for synchronous detection and separation of negative and positive ions based on microchip electrophoresis. The system comprises a micro-fluidic chip, a high-voltage generation control module, a non-contact conductance detection module and a control module. The micro-fluidic chip comprises a microchip cover plate, an insulating layer and a PCB substrate. A micro pipeline is arranged at the bottom of the microchip cover plate. A detection electrode is etched on the top of the PCB substrate. The micro pipeline comprises a linear first channel and a Y-shaped second channel. A sample reservoir and a sample waste liquid tank are arranged at the two ends of the first channel respectively. The second channel comprises a first connecting part, and a second connecting part and a third connecting part which are symmetrically arranged on the front side and the rear side of the tail end of the first connecting part. A buffer solution reservoir is arranged at the head end of the first connecting part; a positive ion reservoir is arranged at the tail end of the second connecting part; and a negative ion reservoir is arranged at the tail end of the third connecting part. According to the system and the method, synchronous detection and separation of positive and negative ions can be realized by only needing the single inlet sample, the micro pipeline and the non-contact conductance detection module.

The invention discloses a photosensitive compound and a preparation method and application thereof, and relates to the technical field of protein nucleic acid co-immobilization, the photosensitive compound is a polyacrylamide compound, and comprises a tetrazole group and a furan group; the compound is prepared from the following raw materials: N-(3-aminopropyl) tert-butyl carbamate, methacryloyl chloride furan-2-yl boric acid, [hydroxyl (phenyl)-lambda 3-iodo] 4-methyl benzene sulfonate and the like. Protein and nucleic acid co-immobilization electrophoresis gel can be prepared, and protein and nucleic acid in single cells or multiple cells can be quantitatively or semi-quantitatively detected; the compound can also be used for microchip electrophoresis mobility analysis, western blotting, nucleic acid blotting hybridization, single-cell western blotting, single-cell northern / source blotting hybridization or capillary electrophoresis western blotting. The protein and nucleic acid co-immobilization gel can be used for simultaneously immobilizing protein and nucleic acid in situ to research the interaction between the protein and DNA (Deoxyribose Nucleic Acid).

The invention provides devices and methods for the detection of hydrophobic biomarkers using 3D microchip capillary electrophoresis having a non-aqueous solvent system. Hydrophobic biomarkers can be placed in a microcapillary microchannel and electrokinetically injected into a second microcapillary microchannel through a nanocapillary array membrane. The hydrophobic biomarkers can then be separated and analyzed via mass spectrometry. Certain hydrophobic biomarkers can indicate a particular disease state.

The invention relates to the technical field of food detection, and discloses a primer combination, a method and a kit for synchronously detecting 14 kinds of animal-derived components in meat or meat products. The present invention uses 10 pairs of specific primers to identify whether pigs, cattle, sheep, goats, chickens, ducks, dogs, foxes, raccoon dogs, cats, and mice are contained in meat or meat products based on two 5-fold PCR reactions and microchip electrophoresis technology. , donkey, deer and horse 14 animal-derived components, the sequences of the 10 pairs of specific primers are shown in SEQ ID NO.1-20. The method of the present invention has the advantages of accurate detection, high sensitivity, strong specificity, simplicity, rapidity and high efficiency, and can satisfy large-scale and rapid identification of whether pigs, cattle, sheep, goats, chickens, ducks, dogs, foxes, etc. are contained in meat or meat products. Requirements for ingredients derived from raccoon dogs, cats, rats, donkeys, deer and horses can effectively resist the adulteration of meat products, effectively protect the interests of consumers, and have good social benefits.

The invention provides a micro-electrophoresis chip and a detection method for online concentration and detection of charged small particles. The micro-electrophoresis chip includes a substrate, three channels are arranged in the substrate, and a plurality of pools are arranged on the substrate, wherein the concentration channel PA, The sample pool P is connected with the concentration pool A; the sampling channel AB is connected with the concentration pool A and the sample waste pool B; the separation channel CD is connected with the buffer pool C and the buffer pool D, and the separation channel CD is connected with the sample injection The channel AB is vertically cross-connected; the above five pools are equipped with high-voltage electrodes for applying high pressure to the pool; the volume of the concentration pool A is adjustable, and the volume of the sample pool P is 10-1000 times the volume of the concentration pool A ; A pair of detection electrodes are provided on the separation channel CD close to the buffer liquid waste pool D, for detecting charged small particles passing through this position. This technology can greatly improve the detection accuracy of the original microchip electrophoresis capacitive coupling non-contact conductometric detection method; while retaining the ME‑C 4 D The advantages of low cost, easy portability, rapid detection, and in-situ detection.

The invention discloses a manufacture method of a detection electrode of a micro-fluidic chip and a preparation method of an electrophoresis non-contact type conductivity detection system. The manufacture method of the compound mold of the micro-fluidic chip comprises the steps of adhering and pressing a sensitive dry film on a substrate under a non-exposed condition, exposing and developing. The preparation method of the detection electrode comprises the steps of adhering and pressing a light-sensitive material on a conductive film layer under the non-exposed condition; exposing; developing; etching; and removing the light-sensitive material. By adopting the manufacture and preparation methods, the compound mold and the corresponding micro-fluidic chip, conductive film electrode and micro-fluidic chip electrophoresis non-contact type conductivity detection system can be obtained in a quick and economical mode.

An end of a capillary storing a sample in its interior is inserted into a sample reservoir in a microchip and positioned near an inlet of a channel connecting to the sample reservoir. Electrodes are placed in the other end of the capillary and a waste reservoir. A predetermined voltage is applied between the electrodes so that a potential is applied between the other end of capillary and waste reservoir. As a result, the sample in the capillary is introduced electrophoretically into a separating medium in the channel. When the sample has been introduced into the channel after the lapse of a specified time, the voltage application between the electrodes is stopped and the capillary is pulled out of the sample reservoir. The sample can be introduced into the channel without placing it in the sample reservoir in a filling amount.

A sample, which is a mixture of glycans, is fluorescently labeled (S2). The sample is subsequently separated by microchip electrophoresis under a buffer solution with no lectin added as well as under multiple kinds of buffer solutions with different lectins respectively added, and the separated components are fluorescently detected (S3). A high-concentration gel which can produce a molecular-sieving effect is used as the buffer solution. Multiple electropherograms are created from the detection results (S4). A glycan having a lectin specifically attached is delayed during its migration in the buffer solution, so that a peak corresponding to this glycan will effectively disappear. Accordingly, based on the kinds of lectins and the presence / absence of a peak on each of the electropherograms, the structure of each glycan in the sample is estimated and the glycan is identified (S5).

A microchip electrophoresis method includes sequentially analyzing a plurality of samples by repeating an analysis process by electrophoresis separation in a microchip, executing a cleaning process of the microchip, and setting timing of the cleaning process of the microchip at arbitrary timing between a plurality of analysis processes.

By using an electrophoretic buffer comprising a polymerized polymer micelle formed by the steps comprising dispersing into an aqueous medium a block copolymer represented by HPLS-HPBS-PLZA, wherein HPLS is a hydrophilic polymer segment, HPBS is a hydrophobic polymer segment, and PLZA is a polymerizable group having an ethylenically unsaturated double bond, and polymerizing the block copolymer as a buffer for a capillary electrophoresis or a microchip electrophoresis, pressurizing the sample after introduction at a given pressure for a given time period, and electrophoresing in an electrophoretic electric field, a polymer compound such as DNA can be separated rapidly and in high separation ability.

The invention discloses a novel large-channel electrophoresis microchip, which is made of PDMS. The chip is provided with a separation channel with a diameter of 0.8-1.2 mm and a total length of 8-30 cm. The two ends of the separation channel are fixed with buffer tanks, and the inside of the separation channel is fixed There is an internal cooling capillary, the two ends of the internal cooling capillary are fixed with hollow fiber membranes, a sampling channel perpendicular to the separation channel is set at a distance of 0.5 to 2 cm from one end of the separation channel, and a sampling channel is set at a distance of 6 to 20 cm from the sampling channel. There is a transfer channel, the transfer channel is perpendicular to the separation channel, and the material is PDMS or quartz capillary. The total length of the chip is 8-30 cm, the width is 2-9 cm, and the chip thickness is 0.2-0.7 cm. Based on the WBE technology, the invention develops a novel large-channel electrophoresis chip with a separation channel size of millimeter level by means of a microfluidic chip. For the first time, the internal cooling module is introduced in the microfluidic chip electrophoresis system, thereby increasing the sample loading to a level equivalent to that of WBE, and at the same time solving the problem of excessive Joule heating effect, and realizing the zero dead volume connection between the large channel and the transfer channel , the whole process of one-dimensional analysis and two-dimensional transfer of samples can be completed through voltage control.

A sample setting section in which at least one sample to be subjected to electrophoresis analysis and at least one standard sample are set, and a controller. The controller includes a standard data storage memory that stores standard data obtained by performing electrophoresis analysis of a standard sample under a predetermined condition, and a chip determination part configured to perform electrophoresis analysis of the standard sample under the predetermined condition using each of the at least one microchip before electrophoresis analysis of the sample set in the sample setting section is performed to acquire analysis data on the standard sample by the detector, and to perform a chip determination as to whether a state of each of the at least one microchip is suitable for performing electrophoresis analysis by comparing the acquired analysis data with the standard data on the standard sample.