Microchip electrophoresis system capable of synchronously detecting negative and positive ions and detection method thereof

Abstract

The invention relates to a microchip electrophoresis system capable of synchronously detecting negative and positive ions and a detection method thereof. The system comprises a micro-fluidic chip, a high-voltage generation control module, a non-contact conductance detection module and a control module. The micro-fluidic chip comprises a microchip cover plate, an insulating layer and a PCB substrate. A micro pipeline is arranged at the bottom of the microchip cover plate. A transmitting electrode, a receiving electrode and a ground electrode are etched on the top of the PCB substrate. The micropipeline comprises a first channel and a second channel which are arranged in a crossed mode and communicated with each other. The two ends of the first channel are provided with a sample reservoir and a sample waste liquid tank respectively. The two ends of the second channel are provided with a buffer solution reservoir and a buffer solution waste liquid tank respectively. The first channel isin a linear shape. The second channel comprises a linear first connecting part, a second connecting part and an arc-shaped third connecting part. According to the system and the method, synchronous detection of positive and negative ions can be realized by only needing the single inlet sample, the micro pipeline and the non-contact conductance detection system.

Description

technical field [0001] The invention relates to the technical field of microchip electrophoresis, in particular to a microchip electrophoresis system capable of synchronously detecting anions and cations and a detection method thereof. Background technique [0002] In the fields of agriculture, environmental monitoring, clinical medical monitoring, food and drug monitoring, etc., the detection objects are often samples with coexistence of cations and anions, such as soil nutrient detection and PM2.5 monitoring. The bioavailable nutrients that plants can absorb in the soil are mainly K + , NH 4 + 、PO 4 3- Wait for anions and cations. PM2.5 is an important part of environmental quality monitoring, it also contains a variety of anions and cations such as F － , Cl － , NO 2 － , NO 3 － , SO 4 2－ 、K + 、Na + , NH 4 + , Ca 2+ , Mg 2+ . The current detection method is to separate the sample, and then separately detect cations and anions, which makes the detection s...

AI Technical Summary

Problems solved by technology

Among them, capillary electrophoresis technology has more detection methods due to its relatively long development time, but this detection technology has many defects compared with microchip electrophoresis detection technology, such as poor separation efficiency, large sample loss, and high pressure required for electrophoresis. Low system integration

However, due to the short development time and high technical difficulty of microchip electrophoresis technology, only three cases have successfully realized the simultaneous detection of anions and cations. One of them uses bipolar isokinetic electrophoresis technology to realize the simultaneous detection of anions and cations, but the detection takes too long , low precision, the second detection method relies on two sets of sampling systems, and in the case of many types of ions to be measured, it is easy to appear aliasing and affect the detection results. The last case uses two sets of separation pipelines and non-contact Conductivity detector, although the short-term detection of anions and cations is realized, the system is very complicated and the cost is too high

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