Device for detecting microchip electrophoresis by time and space resolution and application thereof

A spatio-temporal resolution and detection chip technology, which is applied in measurement devices, material analysis, material analysis by electromagnetic means, etc., can solve the problems of low temporal resolution and spatial resolution, and no simultaneous temporal and spatial resolution.

Inactive Publication Date: 2013-01-23
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the present situation that the detectors currently used in instruments and devices such as capillary electrophoresis, high performance liquid chromatography, and chip electrophoresis do not ha...

Method used

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  • Device for detecting microchip electrophoresis by time and space resolution and application thereof
  • Device for detecting microchip electrophoresis by time and space resolution and application thereof
  • Device for detecting microchip electrophoresis by time and space resolution and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: Construction of the device

[0017] According to the miniaturization requirements of the chip system, the construction of this detection system avoids using complex optical components (mainly lenses, etc.) as much as possible. The whole system is mainly composed of two parts, namely the detection part and the separation part. The separation part consists of a PDMS chip, a high voltage power supply and platinum electrodes. PDMS chips were fabricated according to literature methods. (Refer to Liu, Z.Lab on a Chip.2008,8,1738-1741) The overall size of the chip is 25mm (width)×50mm (length), and the size of the separation channel at the center is 80μm×80μm×28.7mm (width × height × length), there is a liquid reservoir with a diameter of 3mm at each end of the channel, and a metal slit (slit width: 65 μm) is pasted on the bottom of the chip to avoid the interference of stray light. Platinum electrodes are respectively placed in the reservoir at both ends. The...

Embodiment 2

[0018] Example 2: Establishment of isopoint focusing and space-time resolution electropherogram of pure protein (hemoglobin)

[0019] For the preparation of the PDMS microfluidic chip used for detection and the method of equilibrium flushing, refer to the literature (see: Lab chip, 2008 (8): 1738-1741), the samples used are: 1mg / mL hemoglobin, 2% PVP, 2% ampholyte. Electrolyte (the same below): anolyte is 100mmol / L phosphoric acid, 2%PVP; catholyte is 100mmol / L sodium hydroxide, 2%PVP, focusing voltage: 600V, the results are shown in Figure 2. After the original data is directly collected, the following processing is carried out: firstly, the light intensity value is converted into an absorbance value according to Lambert-Beer's law, and then the data is averaged along the vertical direction (that is, the width direction of the CCD), and finally the level of the data is direction (that is, the length direction of the CCD), the wavelet noise reduction is performed, and the obt...

Embodiment 3

[0020] Embodiment 3: the response of riboflavin in different pH value environments

[0021] The sample is 0.1mg / mL riboflavin, 2% PVP, 2% ampholytes. see attached results image 3 .Riboflavin is stable in strong acid solution, and irreversible decomposition is caused by light and ultraviolet radiation. Therefore, the acidic region on the left is focused into a stable peak, and the basic region on the right is decomposed under UV detection. This method can be used to study systems that respond differently to different pH environments.

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Abstract

The invention discloses a device for detecting microchip electrophoresis by time and space resolution. The device consists of two parts comprising a separation part and a detection part, wherein the separation part consists of a PDMS (polydimethylsiloxane) microchip, a high voltage power source and a platinum electrode; the detection part consists of an ultraviolet source, a linear light source converter and a liner detector; the ultraviolet source is linearly converted and covers a whole microchip channel; the linear detector of which the size is similar to that of the microchip channel is used for detecting the whole microchip channel; the linear light source converter, the PDMS microchip and the linear detector are integrated to a dimming light path; optical calibration is performed prior to each time of detection, which is carried out at the position having the strongest signal, and then other parts, such as electrodes, and the like, are connected to a system; and after the detection at each time, relevant data processing is performed according to the requirement of the time and space resolution detection. The device for detecting microchip electrophoresis by the time and space resolution has high time and space resolution, is particularly suitable for representing interaction of biological molecules, and can be used for isoelectrofocusing analysis of protein, and the like.

Description

technical field [0001] The invention relates to a time-space resolution detection chip electrophoresis device and its application in the analysis of biomolecular interaction. Background technique [0002] Detection is one of the cores of analytical instruments. The progress and innovation of many analytical instruments depend on the innovation of detection principle and detection technology. For analytical instruments such as high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE), the most commonly used detectors are ultraviolet absorption detectors, mass spectrometers (MS) and laser-induced fluorescence (LIF), etc., and these detectors are usually placed in A point after the separation column or on the column, that is, "single point detection". For single-point detection, the analyte can only get a corresponding signal when it reaches the detector. Although single-point detection can meet the usual analytical tasks, it has the following disadvan...

Claims

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Application Information

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IPC IPC(8): G01N27/447
Inventor 刘震毕晓东
Owner NANJING UNIV
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