Microelectrophoresis chip and detection method for online concentration and detection of charged small particles

Abstract

The invention provides a micro-electrophoresis chip and a detection method for online concentration and detection of charged small particles. The micro-electrophoresis chip includes a substrate, three channels are arranged in the substrate, and a plurality of pools are arranged on the substrate, wherein the concentration channel PA, The sample pool P is connected with the concentration pool A; the sampling channel AB is connected with the concentration pool A and the sample waste pool B; the separation channel CD is connected with the buffer pool C and the buffer pool D, and the separation channel CD is connected with the sample injection The channel AB is vertically cross-connected; the above five pools are equipped with high-voltage electrodes for applying high pressure to the pool; the volume of the concentration pool A is adjustable, and the volume of the sample pool P is 10-1000 times the volume of the concentration pool A ; A pair of detection electrodes are provided on the separation channel CD close to the buffer liquid waste pool D, for detecting charged small particles passing through this position. This technology can greatly improve the detection accuracy of the original microchip electrophoresis capacitive coupling non-contact conductometric detection method; while retaining the ME‑C 4 D The advantages of low cost, easy portability, rapid detection, and in-situ detection.

Description

Technical field: [0001] The invention relates to a concentration and detection technology of charged small particles, belonging to the field of biochemical analysis instruments and technologies. Background technique: [0002] Small charged particles usually include small inorganic ions such as nutrient ions and heavy metal ions in the soil and water environment, and various small inorganic ions, DNA, proteins and other small charged particles in the human body, animals and plants. ) and the small charged particles produced also belong to the above category. For the processing and detection technologies of these charged small particles, there are currently existing methods such as spectroscopy, mass spectrometry, chromatography, and electrochemical analysis. The above-mentioned methods have disadvantages such as expensive equipment, complicated operation, single function, not easy to be portable, and low quantitative detection accuracy, which make their application scope mos...

AI Technical Summary

Problems solved by technology

The pneumatic valve concentration technology is very ingenious and can be integrated into the microchip, which is well matched with the microchip electrophoresis technology; however, this method additionally introduces a high-precision pneumatic pump, which realizes the closing and closing of the pneumatic valve by accurately injecting and sucking out trace gas. open, which greatly reduces the convenience of the instrument and increases the cost of the instrument

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