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Device and method for leading-in sample into microcrystalline chip electrophoresis

A microchip and electrophoresis technology, applied in sampling devices, microstructure devices, chemical instruments and methods, etc., can solve problems such as reducing sample capacity

Inactive Publication Date: 2007-03-21
SHIMADZU SEISAKUSHO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In related art methods of sample introduction, the volume of the sample must be equal to that of the sample container, which limits efforts to reduce sample volume

Method used

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  • Device and method for leading-in sample into microcrystalline chip electrophoresis
  • Device and method for leading-in sample into microcrystalline chip electrophoresis
  • Device and method for leading-in sample into microcrystalline chip electrophoresis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0032]Fig. 1 is a cross-sectional view of the microchip of the present invention, which shows the X-X section of Figs. 2A to 2C. 1 and 2A to 2C, the following describes the operating steps of the method for introducing a sample into microchip electrophoresis according to the present invention.

[0033] First, in the microchip 1, the grooves 3 and 5 are filled with the separation medium 17, and the containers 7a, 7c, 7s, and 7w are filled with the electrophoresis buffer 19 for power supply. Electrode 21s and 21w are stored in container 7a, 7c, 7s and 7w respectively, and contact with electrophoresis buffer solution 19 for power supply in container 7a, 7c, 7s and 7w, the electrode that is connected with container 7a and 7c is not in Fig. 1 show.

[0034] The sample is stored in the capillary 23 . The capillary 23 is made of a nonconductive material such as glass or resin, and has an outer diameter of 250-365 μm, an inner diameter of 50-100 μm, and a length of 50-70 mm. In thi...

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Abstract

An end of a capillary storing a sample in its interior is inserted into a sample reservoir in a microchip and positioned near an inlet of a channel connecting to the sample reservoir. Electrodes are placed in the other end of the capillary and a waste reservoir. A predetermined voltage is applied between the electrodes so that a potential is applied between the other end of capillary and waste reservoir. As a result, the sample in the capillary is introduced electrophoretically into a separating medium in the channel. When the sample has been introduced into the channel after the lapse of a specified time, the voltage application between the electrodes is stopped and the capillary is pulled out of the sample reservoir. The sample can be introduced into the channel without placing it in the sample reservoir in a filling amount.

Description

technical field [0001] The present invention relates to an apparatus and method for introducing a sample into a microchip electrophoresis, wherein the microchip has grooves formed in the interior of a planar substrate and containers formed at corresponding positions at opposite ends of the grooves, using a microchip The grooves and containers are filled with migration medium during the chip, so that the samples are electrophoresed in the grooves. Background technique [0002] Microchip electrophoresis is usually used to achieve rapid and high-solubility analysis of samples that contain very small amounts of proteins, nucleic acids, and pharmaceuticals. [0003] Electrophoresis techniques have influenced microanalysis of proteins, nucleic acids, etc., of which capillary electrophoresis will be mentioned as a representative example. In capillary electrophoresis, a glass capillary (hereinafter simply referred to as "capillary") having an inner diameter...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447G01N1/10G01N1/00B81B1/00G01N37/00
CPCG01N27/44791G01N27/44743
Inventor 山本林太郎
Owner SHIMADZU SEISAKUSHO CO LTD
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