A combination of primers for the simultaneous detection of 14 animal-derived components in meat or meat products and its application

Abstract

The invention relates to the technical field of food detection, and discloses a primer combination, a method and a kit for synchronously detecting 14 kinds of animal-derived components in meat or meat products. The present invention uses 10 pairs of specific primers to identify whether pigs, cattle, sheep, goats, chickens, ducks, dogs, foxes, raccoon dogs, cats, and mice are contained in meat or meat products based on two 5-fold PCR reactions and microchip electrophoresis technology. , donkey, deer and horse 14 animal-derived components, the sequences of the 10 pairs of specific primers are shown in SEQ ID NO.1-20. The method of the present invention has the advantages of accurate detection, high sensitivity, strong specificity, simplicity, rapidity and high efficiency, and can satisfy large-scale and rapid identification of whether pigs, cattle, sheep, goats, chickens, ducks, dogs, foxes, etc. are contained in meat or meat products. Requirements for ingredients derived from raccoon dogs, cats, rats, donkeys, deer and horses can effectively resist the adulteration of meat products, effectively protect the interests of consumers, and have good social benefits.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a primer combination, a detection method and a kit for identifying 14 kinds of animal-derived components in meat or meat products. Background technique [0002] Meat adulteration has always been a quality problem in the fields of food processing, distribution, and catering. Due to the temptation of interests brought about by price differences, some unscrupulous traders and companies have been driven to adulterate pigs, ducks, and other high-priced meat products such as cattle, sheep, and deer. , chicken and other low-priced meat raw materials. The outbreak of the "horse meat scandal" in Europe in 2013 pushed people's attention to meat adulteration to a climax. This not only seriously violated the interests of consumers, but also caused great harm to the entire meat industry, and more seriously, it caused adverse social impacts. [0003] The detection technology of animal...

AI Technical Summary

Problems solved by technology

Single-plex PCR detects few species, low detection efficiency, and high detection cost, so multiplex PCR technology has gradually become the focus of research

At present, based on multiplex PCR technology, it mainly relies on agarose gel electrophoresis to distinguish PCR amplification products of different lengths. This method has disadvantages and limitations such as complicated process and environmental pollution.

Although the next-generation sequencing technology is gradually applied to the detection of animal-derived components, its popularization and application is limited due to the high cost of instruments and reagents.

Images