Method for extracting limonin substances

A technology for limonoids and an extraction method, which is applied in the field of extraction of limonoid substances, can solve the problems of low content of limonoid substances, serious environmental pollution, danger and the like, and achieves improved release and reduced environmental pollution. , the effect of increasing the output value

Active Publication Date: 2010-01-27
重庆柠柠柒饮料有限公司
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Problems solved by technology

The cost of this extraction method is high, dangerous, serious environ...

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: a kind of extraction method of limonoid substance, it is characterized in that: it is to use lemon seed as raw material, and raw material is first dried and then pulverized to pass through 60 mesh sieves;

[0019] Add 4 times the amount of raw material water, 0.4% cellulase and 0.8% pectinase at 35°C, and the enzymolysis time is 7 hours to extract the enzymolysis solution, which can decompose the cellulose and pectin in the cell wall and make lemon seeds The limonoids in the water are fully released, and at the same time, it is beneficial to hydrolyze the water-soluble limonoid glycosides into limonoid aglycone, which is beneficial to post-processing;

[0020] After the enzymolysis is sufficient, let it stand for 2-4 hours, discard the enzymolysis solution, add 4-6 times the weight of 95% ethanol to the precipitate, stir, mix and extract for 1 hour, collect the filtrate; concentrate the filtrate to a relative temperature of 60°C The density is 1.20-1.30; t...

Embodiment 2

[0022] Embodiment 2: a kind of extraction method of limonoid substance, it is characterized in that: lemon seed is cleaned, dried, pulverized to cross 60~100 mesh sieves, with the water of 3 times raw material amount, add 0.5% cellulase ( 15u / mg) and 0.9% pectinase (1.8u / mg) for enzymolysis at a temperature of 40°C for 6 hours; 5 times the weight of 95% ethanol was stirred and extracted for 1 hour, and the filtrate was collected; when the filtrate was concentrated to 60°C, the relative density was 1.20-1.30; the concentrated filtrate was chromatographed on a macroporous resin column (AB-8 resin), washed with water Remove, initially wash away the water-soluble substances, add ethanol to alcohol content of 20%, 40%, 70%, and collect 70% of the eluent, concentrate the collected 70% eluent under reduced pressure, and the concentrated eluent Then go to silica gel column chromatography, wash with water to equilibrium, elute with 70% ethanol, collect the eluent in sections, and detec...

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PUM

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Abstract

The invention relates to a method for extracting limonin substances from citrus limon seeds as a raw material. The method comprises the following steps: crashing the citrus limon seeds; adding water and an enzyme preparation for enzymolysis; standing; discarding enzymolysis liquid; adding alcohol to sediment for leaching; adopting the absorption chromatographic separation of leaching liquid to obtain the limonin substances; and drying the limonin substances. The method uses lemon product waste, i.e. lemon rods as the raw material so as to not only fully utilize the waste generated in the production process, lessen the waste of the raw material and reduce the environmental pollution, but also obtain the limonin substances with high content up to 95 percent and extraction rate up to 90 percent which is higher than that of the prior extraction rate by 30 percent, and adopts ethanol digestion, macroporous resin column chromatographic separation and silica gel chromatographic column purification to decrease the production cost.

Description

technical field [0001] The invention relates to a method for extracting limonoid substances, in particular to a method for extracting limonoid substances from lemon seeds. Background technique [0002] Limonoids refer to a mixture of limonin and its analogues. The mixture is mainly composed of limonin and nomilin. The product, which mainly exists in a variety of Rutaceae and Meliaceae plants, is also the main substance that causes the bitter taste of citrus juices, and has a wide range of biological activities, such as insecticidal, antiviral, antitumor formation and antibacterial effects. People pay more and more attention to the role of limonoids in anti-cancer, analgesic, regulating cholesterol level, preventing atherosclerosis and deworming, but the relevant research has just started in China. Since limonin and nomilin have similar structures and the same pharmacological effects, they are collectively called limonin substances, and usually the two do not need to be used...

Claims

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Application Information

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IPC IPC(8): A61K36/752A61P33/00A61P31/12A61P35/00A61P31/04A61P29/00A61P9/10A61K131/00A61K31/585
Inventor 谈宗华刘群李天泉张承荣
Owner 重庆柠柠柒饮料有限公司
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