Sample pretreatment method for detecting limonin kind compound content in orange peel and seed raw material

A technology of limonoids and sample pretreatment, which is applied in the preparation of test samples, etc., can solve the problems of health hazards for operators, complex processing process, large amount of solvent, etc. thorough effect

Active Publication Date: 2006-06-28
江门市新会区柑美智慧农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In fact, during the extraction process, the broken raw materials are not evenly dispersed in the solvent, but are deposited at the bottom of the extraction container, and the mass transfer resistance is relatively large, which will also result in long extraction time, large amount of solvent, and incomplete extraction And other issues
[0006] 2) Environmental issues
Repeated heating, extraction and concentration will cause leakage of some solvents, and long-term use will cause harm to the health of the operator
[0008] 3) Operation process and cost
[0009] The general method sample pretreatment process is complicated, time-consuming and reagent-consuming, and the cost is high
Due to the large amount of concentrated solvent, it can still be done with a rotary evaporator in qualified laboratories, but it is difficult for most grassroots factory laboratories to achieve

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take 100g of orange seeds, dry and crush them to 80 mesh, take 9 parallel samples, each sample is 0.2g (accurate to 0.001g), add 0.5ml of limonin standard solution with a concentration of 100ug / ml to 3 of them , according to the following method for sample pretreatment: the sample was degreased with 3ml petroleum ether at high speed for 2min, then centrifuged at 4000r / min for 10min, the supernatant was sucked away, and then 3ml petroleum ether was added to the sample, and this operation was repeated twice Add 3ml of acetone to the degreased sample, shake it at high speed for 2min, centrifuge at 4000r / min for 10min, collect the supernatant, repeat the extraction 3 times, combine the acetone extract, and dry the acetone extract at 40°C; the acetone extract Add 1ml of dichloromethane to the solution, ultrasonically dissolve, centrifuge at 4000r / min for 5min, collect the supernatant, repeat the dissolution 3 times, combine the dichloromethane solution, and dilute to 10ml for...

Embodiment 2

[0027] Take 500g of orange seed raw material, dry and pulverize to 60 mesh, take 3 samples in parallel, each sample is 0.2g (accurate to 0.001g), first degrease with 3ml of petroleum ether at high speed for 2min, then centrifuge at 4000r / min for 10min, Aspirate the supernatant, then add 3ml of petroleum ether to the sample, repeat this operation twice; add 3ml of acetone to the degreased sample, extract by high-speed shaking for 2min, centrifuge at 4000r / min for 10min, collect the supernatant, and repeat the extraction for 3 times , combine the acetone extract, dry the acetone extract at 40°C; add 1ml of chloroform to the acetone extract, shake it at high speed to dissolve, centrifuge at 4000r / min for 5min, collect the supernatant, repeat the dissolution 3 times, and combine Chloroform solution, and dilute to 10ml, to be tested. As in Example 1, the spectrophotometric detection results were respectively 13.02%, 12.95%, 13.07%, with an average of 13.01%.

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Abstract

A sample preprocessing method for detecting lemon bitter principle compound content in citrus skin and seed includes drying and grinding detected sample to be 60 ¿C 80 mu, adding 2 ¿C 5 ml organic solvent into ground sample of 0.2 ¿C 0.5g, oscillating and extracting it for 2 ¿C 3 min. then centrifuging it for 5 ¿C 10 min. to absorb off top clear liquid; adding acetone of 2 ¿C 5ml, extracting and oscillating it for 2 ¿C 3 min. then centrifuging it for 5 ¿C 10min. to collect acetone extractive; adding 1¿C2ml of organic solvent into extractive, oscillating it then centrifuging it for 5 ¿C 10min to collect solution for fixing volume and detecting.

Description

technical field [0001] The invention relates to a sample pretreatment method for detecting the content of limonoid compounds in citrus peel and seed raw materials. Background technique [0002] The biological activity of limonoids in Rutaceae plants (such as citrus, etc.) has received more and more attention, and its application in food and medicine has become more and more extensive. The separation, purification and detection technology of limonoid compounds are also developing continuously. After the raw materials are obtained, their content should be detected first, so as to facilitate the subsequent extraction and purification and product quality control. Therefore, whether the content of limonoid compounds in raw materials can be detected quickly and accurately will directly affect the production efficiency of limonoid. [0003] At present, most of the research work on limonoid compounds revolves around the study of their material structure and functional activity, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/34
Inventor 徐玉娟施英肖更生陈卫东张友胜吴娱明邹宇晓刘学铭
Owner 江门市新会区柑美智慧农业有限公司
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