Method for extracting and purifying antitumor polysaccharide components in sargassum muticum
A purification method and sea millet technology, applied in the direction of anti-tumor drugs, drug combinations, etc., can solve the problem that the anti-tumor ingredients have not been reported, and achieve the effect of significant anti-tumor effect.
Inactive Publication Date: 2013-05-08
YANTAI UNIV
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The research on the active substances of brown algae is very popular. The anti-tumor effect of its extract on mouse tumor cell S180 has been documented long ago, but it has not been reported to obtain its relatively purified anti-tumor components.
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[0032] The specific embodiments of the present invention are given below with reference to the accompanying drawings, which are used to further describe the structure of the present invention.
[0033] A method for extracting and purifying anti-tumor polysaccharide components in sea broomcorns of this embodiment comprises the following steps:
[0034] (1) Extraction of crude polysaccharides: (1) Sea millet is washed to remove impurities, dried and crushed, and passed through a 100-mesh sieve to remove larger particles; (2) The crushed sea millet powder is 90% of its mass (3) Add 2-3L of distilled water to 100g of degreased sea millet powder and extract in a constant temperature water bath at 60-70°C for 4 hours to obtain the extract; (4) Add 2-3L distilled water to the extraction residue and extract again for 4 hours, combine the extracts, and use a vacuum pump to filter and remove solid impurities to obtain a supernatant; (5) Concentrate the supernatant with a vacuum rotary e...
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The invention relates to a method for extracting and purifying antitumor polysaccharide components in sargassum muticum, which belongs to the technical field of extraction and purification methods of antitumor polysaccharide components. The method for extracting and purifying antitumor polysaccharide components in sargassum muticum comprises the following steps: (1) extracting a crude polysaccharide product: carrying out cleaning and impurity removal on sargassum muticum, drying and crushing the sargassum muticum; carrying out quenching, degreasing and room-temperature drying on the sargassum muticum; extracting the obtained product; re-extracting the obtained product, and merging extraction liquid; and carrying out rotary evaporation and graded alcohol precipitation on liquid supernatant; and (2) purifying the crude polysaccharide product: removing impurities in the crude polysaccharide product; preparing freeze-dried powder into an aqueous solution, carrying out chromatography and elution on the aqueous solution; carrying out chromatography and classification on the obtained object so as to obtain three components; and carrying out related pharmacological properties detection. According to the method for extracting and purifying antitumor polysaccharide components in sargassum muticum disclosed by the invention, polysaccharide components with a relative molecular weight in a certain range and containing a certain amount of sulfated groups are obtained, the polysaccharide components have a relatively significant anti-tumor effect, and through antitumor experiments in mice, the polysaccharide components has a regulating effect on the immune system of mice, and lay the foundation for the development of antitumor drugs without toxic and side effects.
Description
[0001] technical field [0002] The invention relates to a method for extracting and purifying anti-tumor polysaccharide components in sea millet, and belongs to the technical field of extraction and purification of anti-tumor polysaccharide components in sea millet. Background technique [0003] Sea millet, seaweed in the intertidal zone, commonly known as corn tassel (Shandong), madder (Fujian), scientific name Sargassum kjellmanianum Yendo . Naturally distributed in the Atlantic coast, the southeast coast of my country, Qingdao, Dalian and other sea areas, it is an important economic seaweed in China. Sea millet has a variety of physiological activities. It is recorded in "Materia Medica Bendu": "Seaweed grows in the sea, and its leaves are like hair. That is, the genus of algae is also a product of salty and cold moistening. Its main function is to soften hard and move water, so all scrofula It can be used for galls, stubborn phlegm and cementation.” Modern pharmacol...
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IPC IPC(8): C08B37/00A61P35/00
Inventor 周革非马伟伟袁平陈丽红邢荣莲滕丽
Owner YANTAI UNIV
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