Single-domain antibody fusion protein and application thereof

A single-domain antibody and fusion protein technology, applied in the field of fusion proteins, can solve the problems of excessive molecular weight, limited therapeutic effect, poor stability, etc., and achieve the effects of good permeability, small molecule and high stability

Active Publication Date: 2018-07-06
NANJING GENSCRIPT BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Even so, existing therapeutic antibodies still have problems such as excessive molecular weight, poor stability, and limited therapeutic effect

Method used

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  • Single-domain antibody fusion protein and application thereof
  • Single-domain antibody fusion protein and application thereof
  • Single-domain antibody fusion protein and application thereof

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Embodiment Construction

[0034] Unless otherwise defined, technical and scientific terms used herein have the meanings commonly understood by one of ordinary skill in the art to which this invention belongs.

[0035] The aforementioned and other purposes and various features of the fusion protein for killing tumor cells of the present invention can be further understood from the following description.

[0036] 1. Antibodies and cytokines:

[0037] Antibody is a large Y-shaped protein secreted by plasma cells (effector B cells) and used by the immune system to identify and neutralize foreign substances such as bacteria and viruses. Monoclonal antibodies are widely used in tumor therapy. Antibodies mentioned in the present invention will include, but are not limited to: those that can specifically bind to tumor-associated antigens and induce immune killing of tumor cells through antibody-dependent cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). Immunoglobulin.

[0038] Single domain ...

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Abstract

The invention provides a single-domain antibody fusion protein. The single-domain antibody fusion protein comprises one or more single-domain antibodies fused with cytokines, in particular one or moreanti-PD-1 single-domain antibodies fused with IL-12 or IL-2. The invention also provides an application of the single-domain antibody fusion protein in preparing medicines for treating tumors. The fusion protein provided by the invention actively combines the advantages of small molecules, good permeability, high stability and the like of single-domain antibodies with the immunoregulation and thelike of the cytokines, and has a better anti-tumor effect.

Description

technical field [0001] The present invention relates to a fusion protein, especially a fusion protein comprising an anti-PD-1 single domain antibody and a cytokine. Background technique [0002] In mammalian cells, the immune system is regulated by immune checkpoints. Two classes of immune regulators are involved in this pathway, including co-stimulatory and co-inhibitory signaling molecules (1). In healthy human cells, the immune system's T-cell activity is suppressed due to tight regulation by immune checkpoint inhibitors, thereby avoiding the devastating effects of autoimmunity and an overactive inflammatory response. However, cancerous cells also use this inhibitory pathway to avoid immune recognition and destruction. To be able to harness the immune system to destroy cancer cells, this immunosuppressive effect needs to be eliminated, thereby removing the inhibition of immune checkpoints (2,3). Currently, monoclonal antibodies that specifically bind inhibitory factors...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C12N15/62A61K39/395A61P35/00
CPCC07K14/535C07K14/5434C07K14/55C07K16/2818A61K2039/505C07K2317/569C07K2317/76C07K2317/92C07K2319/02
Inventor 李中道张亚峰宋立新蒋忻坡张望武术汪东亮柳振宇
Owner NANJING GENSCRIPT BIOTECH CO LTD
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