86results about How to "Has a lethal effect" patented technology

The invention discloses a preparation method and application of a drug-loaded magnetic composite nanometer material. The fat-soluble solution (or emulsion) of the surface lipophilic magnetic nanoparticles and the drug is added to the dichloromethane solution of polylactic acid/glycolic acid copolymer (PLGA), and the three are mixed with each other to form an oil phase system. The oil phase system is added dropwise to the aqueous solution in which the surfactant Pluronic F127 is dissolved to form an oil-in-water system, and then the drug-loaded magnetic composite nanomaterial is obtained under the action of ultrasound. The drug-loaded magnetic composite nanomaterial can concentrate the drug in the tumor area under the action of an in vitro magnetic field to achieve targeting; the coating of PLGA solves the problem of mutual self-aggregation of magnetic particles, and the slow release control of the drug solves the problem It has great toxicity and side effects in the body and can be quickly taken up by tumor cells, leading to apoptosis of tumor cells and reversing the multidrug resistance of tumor cells.

The invention discloses bacillus subtilis and an application thereof to aquaculture, and belongs to the technical field of aquatic microorganisms. The bacillus subtilis NC108 is preserved on April 10, 2017 with the preservation number being CGMCC NO: 14010 at China General Microbiological Culture Collection Center, which is located at No.3 No.1 yard Beichenxilu Chaoyang District, Beijing, andthe preservation is required by Qingdao Agricultural University. The bacillus subtilis NC108 can fast efficiently reduce the content of ammoniacal nitrogen and nitrite in the water body, degrades alga, and has certain antagonism on the skin ulcer syndrome of apostichopus japonicas. The consumption of chemical pesticides and accumulation of chemical pesticides in the water body are reduces, so thatenvironmental pollution is prevented. The bacillus subtilis NC108 is beneficial for improving the quality and yield of aquatic animals and has remarkable economic benefit and social benefit.

The invention provides a Retroactive gene and an application of dsRNA (double-stranded ribonucleic acid) of the Retroactive gene in pest control. Specifically, a Retroactive gene segment is acquired through searching in a migratory locust transcriptome database and is subjected to full-length cloning and sequencing to obtain a full-length cDNA (complementary deoxyribonucleic acid) sequence of the Retroactive gene, a nucleotide sequence and a coded amino acid sequence are SEQ ID NO:1 and SEQ ID NO:2 respectively, a segment with the nucleotide sequence of SEQ ID NO:7 is further designed to synthesize the corresponding dsRNA, the dsRNA is injected into a migratory locust body cavity, the migratory locust cannot exuviate old skin and is dead finally, and the fatality rate is up to 91.2%. The dsRNA has high fatality rate, thereby providing a new molecular target locus for pest control and having significance on effective pest control.

The invention discloses an antibody-coupled drug as well as a preparation and the application thereof. The antibody-coupled drug is obtained from a heresy-base bifunctional-group polyethylene glycol covalent coupled drug molecule and a Fab' fragment, wherein the structure of the antibody-coupled drug is shown in formula I: Fab'-heresy base bifunctional group polyethylene glycol-drug molecule (1), wherein the Fab' is a fragment of an anti-CD20 antibody and the drug molecule is a cytotoxic drug molecule. According to the antibody-coupled drug, the F(ab')2 fragment degraded from the anti-CD20 antibody is reduced to a Fab' fragment of which the hinge area comprises two free thiols; and the Fab' fragment is then covalently coupled with the drug molecule by the heresy-base bifunctional-group polyethylene glycol, so that the antibody-coupled drug is obtained. The antibody-coupled drug prepared by the invention has the characteristics of fixed drug loading ratio and clear drug loading site structure; the antibody-coupled drug also retains the binding capacity of the anti-CD20 antibody to CD20 proteins, so that the antibody-coupled drug has targeting ability to the CD20-positive cells and is applicable to treatment of non-Hodgkin lymphomas.

The invention discloses small molecular polypeptide ZY13 and an application thereof in preparing acne treatment medicine, anti-infection and anti-tumor medicines. The small molecular polypeptide ZY13 comprises 15 amino acid residues, the molecular weight is 2228.77Da, the isoelectric point is 12.05, and the amino acid sequence is represented in SEQID: 1. The polypeptide ZY13 is synthesized artificially, has small molecular weight, is convenient to synthesize artificially, has obvious antibacterial effect, and has remarkable curative effect on acne; the polypeptide can further refrain growth of tumor cells, and has a killing function on the tumor cells; and the small molecular polypeptide ZY13 can be applied to preparation of anti-infection, anti-tumor and acne treatment medicines.

The invention belongs to the technical field of agricultural biology, and in particular relates to a gene silencing technology-based lethal gene fragment Chitinase 7 of laodelphax striatellus and dsRNA (double-stranded RNA) thereof. In the patented invention, a dsRNA feeding method improved in laboratories is used for screening out the lethal gene fragment Chitinase 7 of the laodelphax striatellus as shown in SEQ ID NO. 1 from laodelphax striatellus, wherein the lethal gene fragment Chitinase 7 of the laodelphax striatellus can lead to the death of laodelphax striatellus after being interfered; the dsRNA of the gene fragment is used for feeding the laodelphax striatellus so as to ensure that the corrected death rate of the laodelphax striatellus reaches more than 65%; and the lethal gene fragment Chitinase 7 and the dsRNA thereof provide a sequence and data basis for establishing a new strategy of controlling injurious insects by using a RNA interference technology.

The invention belongs to the technical field of agricultural biology, and in particular relates to gene silencing technology-based lethal gene fragment Chitinase 7 of laodelphax striatellus and dsRNA (double-stranded RNA) thereof. In the patented invention, a dsRNA feeding method improved in laboratories is used for screening out the lethal gene fragment Chitinase 7 of the laodelphax striatellus as shown in SEQ ID NO. 1 from laodelphax striatellus, wherein the lethal gene fragment Chitinase 7 of the laodelphax striatellus can lead to the death of laodelphax striatellus after being interfered; the dsRNA of the gene fragment is used for feeding the laodelphax striatellus so as to ensure that the death rate of the laodelphax striatellus reaches 70%; and the lethal gene fragment Chitinase 7 and the dsRNA thereof provide a sequence and data basis for establishing a new strategy of controlling injurious insects by using an RNA interference technology.

The invention provides a composition, and a preparation method and applications thereof. The composition comprises, by weight, 15 to 30 parts of rosemary essential oil, 1 to 5 parts of Artemisia apiacea extract, and 200 to 300 parts of tea bran extract. The formula is reasonable; synergistic interaction of the components is achieved; in preparation of a scalp nourishing agent, the composition is combined with a plurality of scalp conditioning agents, so that the nourishing effect on scalp is improved; the composition is capable of washing hair, is safe and non-irritant, is capable of preventing alopecia, promoting hair growth, blacking and brightening hair, improving dry and regged hair, is promising in application prospect, and is high in market value.

The invention discloses a pakchoi water culture method. The method comprises the following steps of seed treatment and germination accelerating, sowing, transplanting, management and harvesting. A water culture nutrient solution is prepared by adding 30-50 mL of tofu waste water, 10-20 mL of tea bran water, 20-30 mL of molasses fermentation liquor, 220-250 mg of ammonium nitrate, 5-8 mg of sodium molybdate, 3-5 mg of borax, 3-5 mg of calcium nitrate, 6-10 mg of ferrous sulfate, 1-3 mg of manganese sulfate, 1-3 mg of zinc sulfate and 1-3 mg of copper sulfate into each liter of pigsty liquid dung. The pH value of the nutrient solution is adjusted to be 5.8-6.5 by using citric acid. The electric conductivity is controlled to be 1.5-2.5 mS/ cm, and the dissolved oxygen amount is 4-5 mg/L. Waste water resources are fully utilized, waste is turned into wealth, organic, safe and pollution-free pakchoi is harvested, and the popularization value is relatively high.

The invention provides a polymer drug carrier and a method for preparing the same. The polymer drug carrier is provided with a core-shell structure, a core comprises polyethylene glycol-distearoyl phosphoethanolamine nano-micelle, and a shell comprises poly-dopamine. The method includes forming the nano-micelle from polyethylene glycol-distearoyl phosphoethanolamine; forming the poly-dopamine shell on the outside of the nano-micelle to obtain the polymer drug carrier. The polymer drug carrier and the method have the advantages that the core and the shell of the polymer drug carrier comprise biodegradable polymers, accordingly, the polymer drug carrier is good in biocompatibility, and few toxic and side effects can be realized; the particle size of the polymer drug carrier is smaller than 50nm, tumor gathering effects and photo-thermal therapy effects can be realized, and tumor cell killing effects can be realized after near-infrared light irradiates on the polymer drug carrier; drugs can be encapsulated by the nano-micelle of the polymer drug carrier, diversified drugs can be loaded under chemical modification effects of the poly-dopamine, and accordingly the polymer drug carrier has an excellent application prospect in the field of medicine and pharmacology.

The invention provides an EGFR-CD3 bifunctional antibody. The bifunctional antibody comprises two same light chains and two same recombinant heavy chains, wherein the amino acid sequence of each lightchain is as shown in SEQ ID NO. 8, each recombinant heavy chain is formed by connecting an EGFR antibody heavy chain and a single-chain antibody scFv of CD3 protein through a linker sequence; the amino acid sequence of the EGFR antibody heavy chain is as shown in SEQ ID NO. 7; the amino acid sequence of the linker sequence linker is as shown in SEQ ID NO. 9; the amino acid sequence of the single-chain antibody scFv of the CD3 protein is shown as any one of SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5 or SEQ ID NO.6. The EGFR-CD3 bifunctional antibody can be specifically combined with human CD3 protein and EGFR, the immunogenicity of the murine antibody can be reduced, T cells can be effectively activated, the binding of human EGFR and EGFR can be blocked, the bifunctional antibody can be anchored on the surface of tumor cells by binding with EGFR, and can activate T cells by binding with CD3 so as to achieve the tumor cell killing purpose.

The invention relates to a special feed additive for breeding a laying duck. The special feed additive is prepared from the following raw materials in parts by weight: 2-5 parts of rhizoma atractylodis, 5-7 parts of poria cocos, 2-5 parts of artemisia apiacea, 5-7 parts of cyrtomium fortunei, 2-4 parts of betel nut, 3-5 parts of pericarpium granati, 8-10 parts of fructus psoraleae, 3-5 parts of Chinese wolfberry fruit, 10-15 parts of kelp, 5-8 parts of radix paeoniae alba, 7-11 parts of cockscomb, 10-15 parts of medical stone and 40-50 parts of a carrier. The laying duck is fed by adopting a feed added with the special feed additive provided by the invention, the nutrient uptake and utilization of the laying duck can be promoted so that the egg laying peak season is long, the egg laying quantity is large, the immunity and the disease resistance are enhanced, the survival rate is high, and the economic benefit of a culturing enterprise is remarkably increased.

The invention belongs to the field of medicine and discloses recombinant antibacterial peptide, and a preparation method and an application of the recombinant antibacterial peptide. An amino acid sequence of the recombinant antibacterial peptide is as shown as SEQ ID NO: 1 (Sequence Identifier Number 1). The polypeptide has a better broad-spectrum antibacterial action and can be used for preparing an antibacterial medicine.

The invention discloses anti-tumor oligopeptide B1 sourced from hemocyanin of litopenaeus vannamei. The oligopeptide has an amino acid sequence as shown in SEQ ID NO:1. The oligopeptide can be used for inhibiting growth of cervical cancer cells HeLa, and can become an excellent anti-cancer medicine with low side effect.

The invention discloses a hydroponics nutrient solution for eruca sativa and a preparation method of the hydroponics nutrient solution, wherein a formula of the nutrient solution comprises the following components: 50-80mL of tofu waste water, 50-120mL of tea cake water, 20-30mL of molasses fermentation liquor, 80-100g of plant ash, 0.5-1.5mg of indole butyric acid, 0.5-1.5mg of naphthylacetic acid, 1.5-5mg of chitosan oligosaccharide, 220-250mg of ammonium nitrate, 5-8mg of sodium molybdate, 3-5mg of borax, 3-5mg of calcium nitrate, 6-10mg of ferrous sulfate, 1-3mg of manganese sulfate, 1-3mg of zinc sulfate, 1-3mg of copper sulfate and pig manure water supplemented to 1L. The hydroponics nutrient solution is prepared by the following steps: filtering waste water, heating the filtered waste water, carrying out chelation on the heated waste water and trace elements to form a complex, and regulating a pH value, the conductivity and a dissolved oxygen content finally. The hydroponics nutrient solution is beneficial to growth of the eruca sativa without spraying insecticides and applying a fertilizer any more when being used, is rich in raw material source and simple in preparation technology and has a larger popularization value.

Belonging to the field of agricultural biotechnology, the invention relates to a laodelphax striatellus lethal gene fragment ADP-ribosylation factor based on gene-silencing technology and dsRNA thereof. By a laboratory improved dsRNA feeding method, the invention, from laodelphax striatellus, screens out the laodelphax striatellus lethal gene fragment ADP-ribosylation factor which can lead to laodelphax striatellus death and has sequences as shown in SEQ ID No.1. Feeding laodelphax striatellus with the dsRNA of the gene fragment provided in the invention can have good lethal effect, thus providing sequence and data bases for establishing new strategies of pest control with RNA interference technology.

The invention relates to a Chinese traditional medicine compound glaucescent fissistigma root capsule for curing lung cancer, liver cancer, stomach cancer and esophagus caner. Firstly volatile oil is extracted from zedoary and curcuma root, glaucescent fissistigma root is steeped in water, and solutions are extracted from the residual of zedoary and curcuma root and glaucescent fissistigma root through ethanol; the extracting solutions are mixed and filtrated to obtain a filtrate which is used to recover ethanol and is decompressed and condensed to a clear cream with a relative density of between 1.20 and 1.25 (75 DEG C); then notoginseng is crushed into fine powder and is added into the clear cream after sieving, and the fine powder and the cream are mixed evenly and dried to obtain a dried product; the volatile oil extracted from zedoary and curcuma root is sprayed onto the dried product which is crushed into fine powder and mixed evenly for encapsulation after sieving. The capsule not only has a good curative effect on lung cancer, liver cancer, stomach cancer and esophagus caner with an effectiveness ratio of above 86 percent, but also can reduce the toxic and side effect caused by radiotherapy and chemical therapy. The capsule can obviously alleviate clinical symptoms of a late cancer patient such as distress and stuffiness in the upper abdomen, ache, nausea, eructation, anorexia, bad sleep, lassitude, leucocyte reduction, ascites, bleeding, cancerous cavity, etc.

The invention belongs to the field of biomedicine and discloses application of DC-CIK cells in preparation of a medicine used for resisting HIV infection. Through a lot of experiments, the inventor discovers that when CIK cells impacted by HIV and DC cells are co-cultured, the CIK cells can obviously promote maturation of the DC cells. When the DC and CIK cells are combined for use, the DC and CIK cells have mutual promotion presentation and a function for killing cells infected by HIV. After 30 days of the DC-CIK cell infusion, the number of CD4T cells of a HIV infector is obviously increased, the killing function of NK (Natural Killer) and CIK cells is enhanced, and HIV viral load is obviously restrained. Thus, kill cells induced by co-cultured dendritic cells and cytokines can be applied to the preparation of the medicine used for resisting HIV infection.

A garlicin injection for preventing and treating acute and chronic bacillary dysentery and enteritis, pertussis, fungus infection of lung and digestive tract, etc is prepared through ultrasonic treating to garlicin and tween-80, mixing with alcohol, ultrafiltration, pouring it in containers containing N2 in dark condition, sterilizing, and lamp examining.

The invention discloses a method for extracting an antitumor active substance in fructus forsythiae, the antitumor active substance and an application thereof. The method comprises the following steps: I. preparing fructus forsythiae water extract (FS-1); II preliminarily separating an antitumor effective part (FS-2) by an ethanol precipitation method; III. separating an antitumor effective part (FS-3) of fructus forsythiae by macroporous adsorption resin column chromatography; and IV. separating the antitumor active substance (FS-4) in fructus forsythiae by thin-layer chromatography. Besides, experiments prove that the antitumor active substance (FS-4) in fructus forsythiae can induce apoptosis of a human gastric carcinoma cell SGC-7901. Meanwhile, the specific components in the antitumor active substance (FS-4) in fructus forsythiae are further verified preliminarily. The antitumor active substance (FS-4) in fructus forsythiae, which is extracted by the method, has obvious antitumor activity effects and provides new technological means for treatment of tumor diseases.