Recombinant antibacterial peptide, and preparation method and application of recombinant antibacterial peptide
An antibacterial peptide and antibacterial drug technology, applied in the field of recombinant antibacterial peptide and its preparation, can solve problems such as lack of research, and achieve the effect of good antibacterial effect
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[0040] 1. The polypeptide has the following sequence characteristics:
[0041] Amino acid sequence: ELLNPTHQIYPVTQPLAPVHNPIS (SEQ ID NO: 1)
[0042] Nucleotide sequence:
[0043] GAACTTCTACTTAACCCCACCCACCAGATCTACCCTGTGACTCAGCCACTTGCCCCAGTTCATAACCCCATTAGT (SEQ ID NO: 2)
[0044] It has isoelectric point (pI): 5.99, molecular weight (Mw): 2792.23.
[0045] 2. Establishment of recombinant preparation method
[0046] 2.1 Vector construction:
[0047] The nucleic acid sequence of the polypeptide is artificially synthesized, and the following primers are used by means of PCR technology:
[0048] NO1-F: CATATC GGTCTC GAACTTCTACTTAACCCCA (SEQ ID NO: 3)
[0049] BsaI
[0050] NO1-R: CCC AAGCTT ACTAATGGGGTTATGAACTG (SEQ ID NO: 4)
[0051] Hind III
[0052] The nucleic acid fragment of the polypeptide containing the above restriction site was amplified and recovered by gel, and the recovered product was double digested and loaded into pSUMO vector (L...
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