Recombinant antibacterial peptide, and preparation method and application of recombinant antibacterial peptide

An antibacterial peptide and antibacterial drug technology, applied in the field of recombinant antibacterial peptide and its preparation, can solve problems such as lack of research, and achieve the effect of good antibacterial effect

Inactive Publication Date: 2015-04-29
NANJING MATERNITY & CHILD HEALTH CARE HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on further research on these fragments and their functions in the prior art. Therefore, we screened some polypeptides for in-depth research

Method used

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  • Recombinant antibacterial peptide, and preparation method and application of recombinant antibacterial peptide
  • Recombinant antibacterial peptide, and preparation method and application of recombinant antibacterial peptide
  • Recombinant antibacterial peptide, and preparation method and application of recombinant antibacterial peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. The polypeptide has the following sequence characteristics:

[0041] Amino acid sequence: ELLNPTHQIYPVTQPLAPVHNPIS (SEQ ID NO: 1)

[0042] Nucleotide sequence:

[0043] GAACTTCTACTTAACCCCACCCACCAGATCTACCCTGTGACTCAGCCACTTGCCCCAGTTCATAACCCCATTAGT (SEQ ID NO: 2)

[0044] It has isoelectric point (pI): 5.99, molecular weight (Mw): 2792.23.

[0045] 2. Establishment of recombinant preparation method

[0046] 2.1 Vector construction:

[0047] The nucleic acid sequence of the polypeptide is artificially synthesized, and the following primers are used by means of PCR technology:

[0048] NO1-F: CATATC GGTCTC GAACTTCTACTTAACCCCA (SEQ ID NO: 3)

[0049] BsaI

[0050] NO1-R: CCC AAGCTT ACTAATGGGGTTATGAACTG (SEQ ID NO: 4)

[0051] Hind III

[0052] The nucleic acid fragment of the polypeptide containing the above restriction site was amplified and recovered by gel, and the recovered product was double digested and loaded into pSUMO vector (L...

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Abstract

The invention belongs to the field of medicine and discloses recombinant antibacterial peptide, and a preparation method and an application of the recombinant antibacterial peptide. An amino acid sequence of the recombinant antibacterial peptide is as shown as SEQ ID NO: 1 (Sequence Identifier Number 1). The polypeptide has a better broad-spectrum antibacterial action and can be used for preparing an antibacterial medicine.

Description

technical field [0001] The invention belongs to the field of medicine and relates to a recombinant antibacterial peptide and its preparation method and application. Background technique [0002] After 200 million years of evolution, breastfeeding and its unique product, breast milk, still nourish and protect newborn babies. The large amount of protein in breast milk has an inhibitory effect on pathogenic bacteria, viruses and fungi. Some of these proteins can work alone, while others must work together. It seems that multiple components act on the same pathogenic bacteria as a kind of redundancy, but it is actually a multiple protection system. The hydrolysis of proteases in the body produces a large number of breast milk polypeptides, which not only provide amino acids as nutrition but also perform physiological functions. Further studies have found that a large number of breast milk peptides exhibit multiple antimicrobial effects and provide immune protection for newbor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C12N15/12C12N15/11C12N15/70A61K38/17A61P31/04
CPCC07K14/47A61K38/00C12N15/70
Inventor 崔县伟尤梁惠郭锡熔季晨博陈婷
Owner NANJING MATERNITY & CHILD HEALTH CARE HOSPITAL
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