The invention relates to a disulfiram lipid microsphere preparation for injection for treat tumor and a preparation method thereof, belonging to the field of pharmaceutical preparations. The disulfiram lipid microsphere preparation is prepared from the following components in mass percent: 0.1-4% of disulfiram or derivatives thereof, 5-30% of vegetable oil, 0.6-3% of lecithin, 0-4% of polyethylene glycol phospholipid or derivatives thereof, 0-4% of amino acid block copolymer, 0-0.6% of oleic acid or oleate, 0-1% of antioxidant, 0-0.4% of complexing agent, 1-3% of glycerol and balance of water for injection. According to the invention, due to the introduction of the polyethylene glycol phospholipid or derivatives thereof and the amino acid block copolymer, a fat emulsion can be kept in the blood for a longer time, an additional passive target function on tumor is provided for the medicament, the stability of the fat emulsion preparation is improved, and the effect of disulfiram on treating tumor is enhanced.