Targeted photothermal black phosphorus nano-preparation as well as preparation method and application thereof

A nano-preparation, black phosphorus technology, applied in the field of biomedical nano-materials, can solve the problems of short blood circulation time, the enrichment effect of cancer sites needs to be improved, and poor stability, so as to achieve good target recognition ability and improve photothermal therapy Effect, the effect that the stability is high

Inactive Publication Date: 2017-05-10
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the simple black phosphorus nanomaterials prepared by the existing methods are directly applied to the photothermal therapy of cancer, their stability is poor under physiological conditions, and the blood circulation time is not long, and the enrichment effect at the cancer site needs to be improved

Method used

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  • Targeted photothermal black phosphorus nano-preparation as well as preparation method and application thereof
  • Targeted photothermal black phosphorus nano-preparation as well as preparation method and application thereof
  • Targeted photothermal black phosphorus nano-preparation as well as preparation method and application thereof

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preparation example Construction

[0050] Correspondingly, the embodiment of the present invention also provides a preparation method of targeted photothermal black phosphorus nano-preparation, comprising the following steps:

[0051] (1) Provide black phosphorus nanoflakes;

[0052] (2) Add hydroxysuccinimide-activated folic acid to the dimethyl sulfoxide solution of diamino-terminated polyethylene glycol, and add an appropriate amount of catalyst to obtain a mixed solution, which is carried out at room temperature and under dark conditions. Stir the reaction for 16-32 hours, filter after the reaction is completed, and obtain folic acid-modified polyethylene glycol, the folic acid is connected to the polyethylene glycol through an amide bond, one end of the polyethylene glycol is an amino group, and the other end is an ethylene glycol. Amino group, the nitrogen atom of the imino group is connected with the carbonyl carbon atom in the folic acid to form the amide bond;

[0053] (3) Using probe ultrasound and m...

Embodiment 1

[0069] A method for preparing a targeted photothermal black phosphorus nano-preparation, comprising the following steps:

[0070] (1) Preparation of black phosphorus nanoflakes by solution stripping combined with probe ultrasonic method: take 50 mg block black phosphorus material, place it in 100 mL ultrapure water, and carry out probe ultrasonic, and the ultrasonic time of the probe ultrasonic method is 12 hours , the process is continuous ultrasonication for 45 seconds, waiting for 15 seconds as a cycle, and the power amplifier is 20%; after the ultrasonication is over, take the brown-black stripping solution and put it in a centrifuge tube, centrifuge at 1000rpm for 10 minutes at 4°C, and the precipitate has not been stripped block black phosphorus, and carefully separate the precipitated block black phosphorus and the black phosphorus nanoflakes in the supernatant to further purify and separate the black phosphorus nanoflakes;

[0071] (2) Add triethylamine (0.5mL), NHS (0...

Embodiment 2

[0080] A method for preparing a targeted photothermal black phosphorus nano-preparation, comprising the following steps:

[0081] (1) Preparation of black phosphorus nanoflakes by solution stripping combined with probe ultrasonic method: take 50 mg block black phosphorus material, place it in 100 mL ultrapure water, and carry out probe ultrasonic, and the ultrasonic time of the probe ultrasonic method is 18 hours , the process is continuous ultrasound for 1 hour, waiting for 1 hour as a cycle, and the power amplifier is 20%. After the ultrasound is over, take the brown-black stripping solution and put it in a centrifuge tube, centrifuge at 2000rpm for 6 minutes at 4°C to precipitate the unstripped massive black phosphorus, and carefully separate the precipitated massive black phosphorus from the supernatant Black phosphorus nano-flakes for further purification and separation of black phosphorus nano-flakes;

[0082] (2) Add triethylamine (0.5mL), NHS (0.52g, 5.00mmol) and DCC...

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Abstract

The invention provides a targeted photothermal black phosphorus nano-preparation, which is prepared from a black phosphorus nano-slice, polyethylene glycol absorbed on the surface of the black phosphorus nano-slice by means of electrostatic attraction, and folic acid connected to the polyethylene glycol by means of an amide bond, wherein one end of the polyethylene glycol is amino, and the other end of the polyethylene glycol is imino; nitrogen atom of the imino and carbonyl carbon atom of the folic acid are connected with each other so as to form the amide bond; the folic acid is exposed at the outermost layer of the nano-preparation. The targeted photothermal black phosphorus nano-preparation is high in stability, good in target recognition ability and excellent in light-heat property, is capable of effectively killing cancer cells, and can be used for performing targeted photothermal treatment on cancers at the cellular level. The invention also provides a preparation method and application of the targeted photothermal black phosphorus nano-preparation.

Description

technical field [0001] The invention relates to the technical field of biomedical nanomaterials, in particular to a targeted photothermal black phosphorus nano-preparation and its preparation method and application. Background technique [0002] As one of the major malignant diseases that threaten human health, the treatment of cancer, whether it is chemotherapy, surgery or radiotherapy, has great side effects on the body, and after malignant metastasis occurs, no matter which method is used, it is difficult to completely cure. Although a lot of human, material and financial resources have been invested in the research of cancer, the progress is very limited, and its effective treatment is still a great test for human beings. [0003] Studies have shown that the application of nanotechnology to the field of cancer diagnosis and treatment has broad prospects and clinical value. As a new type of cancer treatment, photothermal therapy uses the thermal effect generated by phot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K47/60A61P35/00
CPCA61K41/0052
Inventor 张晗陶伟张家宜
Owner SHENZHEN UNIV
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