Method for extracting antitumor active substance in fructus forsythiae, antitumor active substance and application thereof

An anti-tumor activity and anti-tumor technology, applied in the field of biomedicine, can solve the problems of high side effects and easy drug resistance of chemotherapy drugs

Active Publication Date: 2014-07-23
HARBIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of large side effects and drug resistance of chemotherapeutic drugs existing in the clinical treatment of cancer, especially gastric cancer, the present invention uses the Chinese herbal medicine Forsythia forsythia (i.e. the dried Fruit) as the research object, it was separated and extracted to obtain the anti-tumor active substance of Forsythia (FS-4), and its anti-tumor activity was measured, and then a kind of anti-tumor substance isolated and extracted from Forsythia was provided. Method of action of antineoplastic active substances of forsythia

Method used

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  • Method for extracting antitumor active substance in fructus forsythiae, antitumor active substance and application thereof
  • Method for extracting antitumor active substance in fructus forsythiae, antitumor active substance and application thereof
  • Method for extracting antitumor active substance in fructus forsythiae, antitumor active substance and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0042] Example 1: Preparation of Forsythia Water Extract (FS-1)

[0043] Take 500g of forsythia (purchased from Shiyitang pharmacy, Huangqiao, place of production in Shanxi), sieve and remove dust, wash 5 times with tap water, wash 3 times with distilled water, add distilled water to make the liquid level about 10cm higher than forsythia, soak overnight. Boil at 100°C the next day, filter while hot with absorbent cotton for 2 hours, extract the dregs repeatedly, then filter with absorbent cotton, mix the two filtrates, evaporate and concentrate at 100°C to 1.00 mg / ml, and press them out intermittently for three days Sterilize by bacterial method, 40min each time, make water extract, mark it as FS-1, and store it in a refrigerator at 4°C for later use.

Embodiment 2

[0044] Example 2: Preliminary isolation of effective anti-tumor fraction by ethanol precipitation method (FS-2)

[0045]Take 3.5ml of forsythia water extract FS-1 and place it in a plastic centrifuge tube, then slowly add ethanol solution with a mass percentage concentration of 100% to it in sections, and keep shaking until the volume of ethanol solution reaches 90% of the total volume of the solution until. Put the centrifuge tube in a 4°C refrigerator for 12 hours, centrifuge in a low-temperature centrifuge at 4°C, 4000r / min for 15min, collect the supernatant, filter the supernatant through filter paper, concentrate under reduced pressure, put it in a 4°C refrigerator, and set aside, labeled as FS- 2.

Embodiment 3

[0046] Example 3: Separation of Forsythia antitumor effective fraction (FS-3) by macroporous adsorption resin column chromatography

[0047] Take FS-2 and slowly add it to the top of the pretreated macroporous adsorption resin (X-5 type) column, the addition amount is 2% of the column volume, 8mg / time, 6 times in total. After FS-2 penetrates into the resin layer, elute with distilled water, ethanol solutions with a mass percentage concentration of 30%, 60%, and 90% in sequence at a flow rate of 5.00ml / min, collect the eluted part, and use a mass percentage concentration of 90% ethanol solution The eluate from ethanol solution was concentrated under reduced pressure at 100°C to obtain FS-3.

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Abstract

The invention discloses a method for extracting an antitumor active substance in fructus forsythiae, the antitumor active substance and an application thereof. The method comprises the following steps: I. preparing fructus forsythiae water extract (FS-1); II preliminarily separating an antitumor effective part (FS-2) by an ethanol precipitation method; III. separating an antitumor effective part (FS-3) of fructus forsythiae by macroporous adsorption resin column chromatography; and IV. separating the antitumor active substance (FS-4) in fructus forsythiae by thin-layer chromatography. Besides, experiments prove that the antitumor active substance (FS-4) in fructus forsythiae can induce apoptosis of a human gastric carcinoma cell SGC-7901. Meanwhile, the specific components in the antitumor active substance (FS-4) in fructus forsythiae are further verified preliminarily. The antitumor active substance (FS-4) in fructus forsythiae, which is extracted by the method, has obvious antitumor activity effects and provides new technological means for treatment of tumor diseases.

Description

technical field [0001] The present invention relates to a method for extracting active substances and its products and applications, in particular to a method for extracting antitumor active substances from Forsythia japonicus and the product extracted by the method and the application of the product in antitumor. The invention belongs to the technical field of biomedicine. Background technique [0002] Forsythia is one of the traditional Chinese medicines commonly used in clinical practice in China, also known as Huanghuatiao, Lianhu, Qingqiao, Luoqiao, Huangqidan, etc. Forsythia (Forsythia suspensa (Thunb.) Vahl) in the Pharmacopoeia of the People's Republic of China is the dried fruit of Forsythia suspensa (Thunb.) Vahl in the family Oleaceae (Oleaceae). Forsythia is commonly used to treat acute wind-heat cold, carbuncle sore, lymph node tuberculosis, urinary tract infection and other diseases. [0003] Gastric cancer is currently one of the most common malignant tumors...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/634A61P35/00
Inventor 李鑫刘微李洪源李丹娜
Owner HARBIN MEDICAL UNIVERSITY
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