Method for biotransformation production of gamma-aminobutyric acid with aquatic products and processing leftovers thereof as raw materials

A biotransformation and aminobutyric acid technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high price, shallow research on separation and purification, and low purity of GABA, so as to save costs and reduce Environmental pollution and the effect of improving the economic benefits of enterprises

Active Publication Date: 2014-08-06
中科海洋生物研究院盘锦有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In 2009, the Ministry of Health of my country approved γ-aminobutyric acid as a new resource food. However, at present, the GABA used in my country mainly relies on imports. The domestic extraction process of GABA is not yet mature, and the research on separation and purification is still shallow, and the purity of GABA obtained is relatively low. , and there is no standard detection method for GABA content in food
Aquatic products are rich in amino acids, especially some flavor amino acids, such as glutamic acid, which is used as a raw material for the production of GABA. At present, only one research institute in Korea has studied it, but it is produced by fermenting and cultivating expensive MRS medium. However, using the self-fermentation product combined with sodium chloride, glucose and other C sources and N sources as the medium to produce GABA through step-by-step supplementation of glutamic acid or glutamate, there have been no reports at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1) After 100g of oysters are fermented for 4 months, filter, take 100ml of fermented liquid, add 2% glucose, 1.5% sodium chloride, 0.3% phosphate, distill the volume to 500ml with distilled water, put it into a three-necked bottle, and keep it at 115℃ Sterilize for 20 minutes, take it out and cool it for later use after the sterilization is completed.

[0032] 2) Biotransformation of lactic acid bacteria: Inoculate Bifidobacterium longum with glutamic acid decarboxylase activity into the above-mentioned Erlenmeyer flask containing the medium at a ratio of 1 (V / V), control the fermentation temperature at 34°C, and the rotation speed of the fermentation shaker flask 130r / min, cultivate for 24 hours, add 1% sodium glutamate every 6 hours during the fermentation process, and carry out the GABA fermentation culture of aquatic products and their processing waste biotransformation;

[0033] 3) Quantitative detection of products: stop the fermentation of the lactic acid bacteri...

Embodiment 2

[0036] 1) After 100g of oysters were fermented for 4 months, filter, take 100ml of fermented liquid, add 2% glucose, 1.5% sodium chloride, 0.3% phosphate to it, dilute to 500ml with distilled water, put it into a three-neck bottle, Sterilize at 115°C for 20 minutes. After the sterilization is completed, take it out and cool it for later use.

[0037] 2) Biotransformation of lactic acid bacteria: Inoculate Bifidobacterium longum with glutamic acid decarboxylase activity into the above-mentioned Erlenmeyer flask containing the medium at a ratio of 1 (V / V), control the fermentation temperature at 35°C, and the rotation speed of the fermentation shaker flask 150r / min, cultivate for 28 hours, add 2% sodium glutamate every 7 hours during the fermentation process, and carry out the GABA fermentation culture of aquatic products and their processing waste biotransformation;

[0038] 3) Obtaining the product: stop the fermentation of the lactic acid bacteria fermentation liquid that has...

Embodiment 3

[0042] 1) After 100g of scallop scraps (skirt and viscera mixture) fermented for 5 months, filter, take 100ml of fermentation broth, add 2% glucose, 1.5% sodium chloride, 0.3% phosphate, and distilled water to volume 500ml, put it into a three-neck bottle, sterilize at 115°C for 20 minutes, take it out and cool it for later use. 2) Biotransformation of lactic acid bacteria: Inoculate Bifidobacterium longum with glutamic acid decarboxylase activity into the above-mentioned Erlenmeyer flask containing the medium at a ratio of 1 (V / V), control the fermentation temperature at 36°C, and the rotation speed of the fermentation shaker flask 200r / min, cultivate for 30 hours, add 3% sodium glutamate every 6 hours during the fermentation process, and carry out the GABA fermentation culture of aquatic products and their processing waste biotransformation;

[0043] 3) Obtaining the product: stop the fermentation of the lactic acid bacteria fermentation liquid that has been fermented for 24...

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PUM

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Abstract

The invention specifically relates to a method for biotransformation production of gamma-aminobutyric acid by using aquatic products and processing leftovers thereof, which belongs to the technical field of bio-processing of aquatic products. The method comprises the following steps: inoculating 0.5 to 5% (V / V) of lactic acid bacteria with glutamic acid decarboxylase activity into a medium, carrying out fermentation culture at a fermentation temperature of 30 to 35 DEG C at a rotating speed of 100 to 500 r / min for 20 to 50 h so as to allow a viable count in fermentation broth to be as high as 10<6> to 10<7> cfu / ml and standing the fermented lactic acid bacteria for subsequent usage; carrying out centrifugation or filtering on transformation liquid, taking supernatant and subjecting the supernatant to reduced pressure concentration so as to prepare a GABA crude extract; and removing impurities in the crude extract with absolute ethyl alcohol by using a precipitation method, then carrying out separation and purification by successively using a macroporous resin, Sephadex gel and a cation exchange resin and subjecting a GABA product obtained after separation and purification to rotary evaporation and freeze drying so as to obtain a crystal. With the method, a novel application direction for aquatic products and processing leftovers thereof is opened up, and theoretical bases are provided for comprehensive and high-value utilization of aquatic products.

Description

technical field [0001] The invention belongs to the technical field of biological processing of aquatic products, and in particular relates to a method for producing gamma-aminobutyric acid through biotransformation of aquatic products and their processing leftovers. Background technique [0002] γ-Aminobutiric acid (GABA) is an important inhibitory neurotransmitter in the nervous system of mammals, crustaceans, insects and some parasitic worms. A small number of plants contain this non-protein amino acid. γ-aminobutyric acid, also known as aminobutyric acid, is a natural amino acid that is not a protein component. It exists widely in nature and is an important inhibitory neurotransmitter that has been studied in depth. It participates in various metabolic activities. It has the effects of lowering blood pressure, regulating arrhythmia, improving sleep, anti-anxiety, improving lipid metabolism, and preventing arteriosclerosis, so it has attracted the attention of more and m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00C12R1/01
Inventor 邢荣娥李鹏程杨皓月李冰刘松于华华秦玉坤陈晓琳
Owner 中科海洋生物研究院盘锦有限公司
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